Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01005 and MMP_RS01010 are separated by 128 nucleotides MMP_RS01010 and MMP_RS01015 are separated by 250 nucleotides MMP_RS01015 and MMP_RS01020 are separated by 170 nucleotides
MMP_RS01005: MMP_RS01005 - riboflavin synthase, at 190,641 to 191,111
_RS01005
MMP_RS01010: MMP_RS01010 - DUF4352 domain-containing protein, at 191,240 to 191,773
_RS01010
MMP_RS01015: MMP_RS01015 - hypothetical protein, at 192,024 to 192,236
_RS01015
MMP_RS01020: MMP_RS01020 - 3 4-dihydroxy-2-butanone-4-phosphate synthase, at 192,407 to 193,150
_RS01020
Position (kb) 192
193 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 191.097 kb on - strand at 191.144 kb on - strand at 191.151 kb on - strand at 191.152 kb on + strand at 191.186 kb on - strand at 191.191 kb on - strand at 191.273 kb on - strand at 191.273 kb on - strand at 191.274 kb on + strand at 191.280 kb on - strand at 191.281 kb on + strand at 191.303 kb on + strand, within MMP_RS01010 at 191.309 kb on - strand, within MMP_RS01010 at 191.396 kb on - strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.442 kb on + strand, within MMP_RS01010 at 191.478 kb on + strand, within MMP_RS01010 at 191.519 kb on + strand, within MMP_RS01010 at 191.522 kb on - strand, within MMP_RS01010 at 191.523 kb on + strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.715 kb on + strand, within MMP_RS01010 at 191.740 kb on - strand at 191.750 kb on - strand at 191.750 kb on - strand at 191.753 kb on - strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.755 kb on - strand at 191.756 kb on + strand at 191.779 kb on - strand at 191.780 kb on + strand at 191.781 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.801 kb on + strand at 191.801 kb on + strand at 191.841 kb on + strand at 191.852 kb on + strand at 191.854 kb on - strand at 191.855 kb on + strand at 191.871 kb on + strand at 191.874 kb on - strand at 191.875 kb on + strand at 191.912 kb on - strand at 191.993 kb on - strand at 191.994 kb on + strand at 192.305 kb on - strand at 192.321 kb on - strand at 192.749 kb on + strand, within MMP_RS01020 at 193.106 kb on - strand at 193.107 kb on + strand at 193.165 kb on + strand at 193.170 kb on - strand at 193.193 kb on + strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.200 kb on - strand at 193.200 kb on - strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.203 kb on + strand at 193.229 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 191,097 - -3.6 191,144 - -0.0 191,151 - +0.3 191,152 + -2.5 191,186 - +0.7 191,191 - -0.5 191,273 - -0.4 191,273 - -0.4 191,274 + +0.3 191,280 - -0.2 191,281 + +0.3 191,303 + MMP_RS01010 0.12 +1.4 191,309 - MMP_RS01010 0.13 +0.8 191,396 - MMP_RS01010 0.29 +0.6 191,397 + MMP_RS01010 0.29 +0.2 191,397 + MMP_RS01010 0.29 -0.5 191,442 + MMP_RS01010 0.38 -1.1 191,478 + MMP_RS01010 0.45 +0.1 191,519 + MMP_RS01010 0.52 +0.1 191,522 - MMP_RS01010 0.53 +1.2 191,523 + MMP_RS01010 0.53 +0.2 191,572 - MMP_RS01010 0.62 -0.8 191,572 - MMP_RS01010 0.62 -0.4 191,715 + MMP_RS01010 0.89 -1.0 191,740 - +0.8 191,750 - +0.6 191,750 - +0.7 191,753 - -1.2 191,754 + -0.0 191,754 + -1.7 191,754 + -0.6 191,755 - +0.0 191,756 + +0.0 191,779 - -0.4 191,780 + -0.3 191,781 - +0.0 191,795 - -0.4 191,795 - +0.5 191,795 - +0.8 191,801 + -0.9 191,801 + +1.1 191,841 + -0.1 191,852 + +0.4 191,854 - +0.5 191,855 + +0.3 191,871 + -0.0 191,874 - +0.1 191,875 + +0.4 191,912 - +0.0 191,993 - -0.5 191,994 + +0.6 192,305 - -0.2 192,321 - +0.2 192,749 + MMP_RS01020 0.46 -4.5 193,106 - -3.7 193,107 + -2.5 193,165 + -0.9 193,170 - -0.2 193,193 + +0.4 193,198 - -0.1 193,198 - -1.0 193,198 - +0.2 193,199 + +0.4 193,199 + +0.5 193,199 + +1.3 193,199 + +0.2 193,199 + -0.4 193,200 - +1.3 193,200 - -0.0 193,201 + +0.6 193,201 + -0.2 193,201 + +0.9 193,201 + +0.7 193,201 + +1.3 193,201 + +1.2 193,203 + -0.4 193,229 - +0.2
Or see this region's nucleotide sequence