Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00710 and MMP_RS00715 are separated by 180 nucleotides MMP_RS00715 and MMP_RS00720 are separated by 27 nucleotides MMP_RS00720 and MMP_RS00725 are separated by 40 nucleotides
MMP_RS00710: MMP_RS00710 - hypothetical protein, at 127,981 to 128,889
_RS00710
MMP_RS00715: MMP_RS00715 - formate-dependent phosphoribosylglycinamide formyltransferase, at 129,070 to 130,236
_RS00715
MMP_RS00720: MMP_RS00720 - metal-dependent hydrolase, at 130,264 to 130,938
_RS00720
MMP_RS00725: MMP_RS00725 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 130,979 to 132,511
_RS00725
Position (kb) 129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 128.205 kb on - strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.777 kb on + strand, within MMP_RS00710 at 128.800 kb on - strand at 128.800 kb on - strand at 128.829 kb on - strand at 128.858 kb on + strand at 128.858 kb on + strand at 128.859 kb on - strand at 128.859 kb on - strand at 128.967 kb on - strand at 128.989 kb on - strand at 128.990 kb on + strand at 128.995 kb on + strand at 128.996 kb on - strand at 128.996 kb on - strand at 128.996 kb on - strand at 128.999 kb on - strand at 129.001 kb on - strand at 129.028 kb on - strand at 129.225 kb on - strand at 129.638 kb on - strand at 129.875 kb on - strand at 130.010 kb on - strand at 130.115 kb on - strand at 130.146 kb on - strand at 130.284 kb on + strand at 130.292 kb on + strand at 130.477 kb on + strand, within MMP_RS00720 at 130.534 kb on + strand, within MMP_RS00720 at 130.650 kb on - strand, within MMP_RS00720 at 130.683 kb on - strand, within MMP_RS00720 at 130.684 kb on + strand, within MMP_RS00720 at 130.685 kb on - strand, within MMP_RS00720 at 130.748 kb on + strand, within MMP_RS00720 at 130.751 kb on - strand, within MMP_RS00720 at 130.752 kb on + strand, within MMP_RS00720 at 130.773 kb on - strand, within MMP_RS00720 at 130.790 kb on - strand, within MMP_RS00720 at 130.791 kb on + strand, within MMP_RS00720 at 130.807 kb on - strand, within MMP_RS00720 at 130.808 kb on + strand, within MMP_RS00720 at 130.819 kb on - strand, within MMP_RS00720 at 130.831 kb on - strand, within MMP_RS00720 at 130.887 kb on - strand at 130.907 kb on + strand at 130.918 kb on + strand at 130.986 kb on + strand at 131.041 kb on - strand at 131.092 kb on - strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.101 kb on + strand at 131.123 kb on - strand at 131.173 kb on + strand, within MMP_RS00725 at 131.173 kb on + strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.174 kb on - strand, within MMP_RS00725 at 131.175 kb on + strand, within MMP_RS00725
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 128,205 - MMP_RS00710 0.25 -0.2 128,546 + MMP_RS00710 0.62 -0.9 128,546 + MMP_RS00710 0.62 +0.5 128,777 + MMP_RS00710 0.88 +0.8 128,800 - +0.0 128,800 - +0.6 128,829 - +0.2 128,858 + +0.1 128,858 + +0.3 128,859 - -0.7 128,859 - +0.7 128,967 - -0.9 128,989 - -0.2 128,990 + +0.2 128,995 + +0.9 128,996 - +0.2 128,996 - +0.1 128,996 - -0.3 128,999 - -0.7 129,001 - -0.0 129,028 - -2.2 129,225 - -1.9 129,638 - -2.2 129,875 - -2.1 130,010 - -2.5 130,115 - -0.9 130,146 - -1.4 130,284 + -0.5 130,292 + -0.3 130,477 + MMP_RS00720 0.32 -0.8 130,534 + MMP_RS00720 0.40 -0.6 130,650 - MMP_RS00720 0.57 -1.0 130,683 - MMP_RS00720 0.62 -2.5 130,684 + MMP_RS00720 0.62 -0.8 130,685 - MMP_RS00720 0.62 -1.1 130,748 + MMP_RS00720 0.72 -0.2 130,751 - MMP_RS00720 0.72 -0.5 130,752 + MMP_RS00720 0.72 -3.2 130,773 - MMP_RS00720 0.75 -0.6 130,790 - MMP_RS00720 0.78 -1.7 130,791 + MMP_RS00720 0.78 -1.0 130,807 - MMP_RS00720 0.80 -1.3 130,808 + MMP_RS00720 0.81 -2.3 130,819 - MMP_RS00720 0.82 -1.3 130,831 - MMP_RS00720 0.84 -0.4 130,887 - -0.4 130,907 + -0.6 130,918 + +0.5 130,986 + -0.7 131,041 - -3.3 131,092 - -3.6 131,101 + -2.6 131,101 + -1.8 131,101 + -3.1 131,123 - -1.8 131,173 + MMP_RS00725 0.13 -2.6 131,173 + MMP_RS00725 0.13 -3.0 131,174 - MMP_RS00725 0.13 -1.3 131,174 - MMP_RS00725 0.13 -2.0 131,175 + MMP_RS00725 0.13 -1.9
Or see this region's nucleotide sequence