Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00565 and MMP_RS00570 are separated by 88 nucleotides MMP_RS00570 and MMP_RS00575 are separated by 118 nucleotides MMP_RS00575 and MMP_RS00580 are separated by 157 nucleotides MMP_RS00580 and MMP_RS00585 are separated by 46 nucleotides MMP_RS00585 and MMP_RS00590 are separated by 8 nucleotides MMP_RS00590 and MMP_RS00595 are separated by 16 nucleotides MMP_RS00595 and MMP_RS00600 are separated by 40 nucleotides MMP_RS00600 and MMP_RS00605 are separated by 16 nucleotides MMP_RS00605 and MMP_RS00610 are separated by 33 nucleotides MMP_RS00610 and MMP_RS00615 are separated by 20 nucleotides
MMP_RS00565: MMP_RS00565 - CBS domain-containing protein, at 110,490 to 110,975
_RS00565
MMP_RS00570: MMP_RS00570 - protein-L-isoaspartate O-methyltransferase, at 111,064 to 111,702
_RS00570
MMP_RS00575: MMP_RS00575 - pyridoxal 5'-phosphate synthase lyase subunit PdxS, at 111,821 to 112,720
_RS00575
MMP_RS00580: MMP_RS00580 - tRNA-Thr, at 112,878 to 112,951
_RS00580
MMP_RS00585: MMP_RS00585 - tRNA-Pro, at 112,998 to 113,074
_RS00585
MMP_RS00590: MMP_RS00590 - tRNA-Tyr, at 113,083 to 113,156
_RS00590
MMP_RS00595: MMP_RS00595 - tRNA-Lys, at 113,173 to 113,246
_RS00595
MMP_RS00600: MMP_RS00600 - 5S ribosomal RNA, at 113,287 to 113,401
_RS00600
MMP_RS00605: MMP_RS00605 - tRNA-Asp, at 113,418 to 113,492
_RS00605
MMP_RS00610: MMP_RS00610 - tRNA-Lys, at 113,526 to 113,599
_RS00610
MMP_RS00615: MMP_RS00615 - tRNA-Asp, at 113,620 to 113,694
_RS00615
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 110.823 kb on - strand, within MMP_RS00565 at 110.882 kb on - strand, within MMP_RS00565 at 110.934 kb on + strand at 110.944 kb on - strand at 111.004 kb on - strand at 111.004 kb on - strand at 111.005 kb on + strand at 111.005 kb on + strand at 111.006 kb on - strand at 111.006 kb on - strand at 111.006 kb on - strand at 111.007 kb on + strand at 111.007 kb on + strand at 111.007 kb on + strand at 111.007 kb on + strand at 111.007 kb on + strand at 111.022 kb on - strand at 111.024 kb on - strand at 111.024 kb on - strand at 111.025 kb on + strand at 111.025 kb on + strand at 111.025 kb on + strand at 111.061 kb on - strand at 111.061 kb on - strand at 111.072 kb on + strand at 111.072 kb on + strand at 111.115 kb on - strand at 111.237 kb on - strand, within MMP_RS00570 at 111.302 kb on - strand, within MMP_RS00570 at 111.700 kb on - strand at 111.786 kb on - strand at 111.789 kb on - strand at 112.635 kb on - strand at 112.669 kb on - strand at 112.734 kb on - strand at 112.734 kb on - strand at 112.751 kb on - strand at 112.752 kb on + strand at 112.777 kb on - strand at 112.798 kb on - strand at 112.798 kb on - strand at 112.799 kb on + strand at 112.799 kb on + strand at 112.809 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 110,823 - MMP_RS00565 0.69 +0.7 110,882 - MMP_RS00565 0.81 +1.0 110,934 + +1.5 110,944 - -0.1 111,004 - +0.8 111,004 - -1.5 111,005 + +0.6 111,005 + -0.2 111,006 - +0.8 111,006 - -0.1 111,006 - +0.2 111,007 + +0.8 111,007 + +0.8 111,007 + +0.6 111,007 + -0.4 111,007 + -1.9 111,022 - +1.8 111,024 - -0.4 111,024 - +0.5 111,025 + +1.0 111,025 + -1.3 111,025 + -0.4 111,061 - -0.0 111,061 - +1.0 111,072 + -0.2 111,072 + -0.0 111,115 - +0.8 111,237 - MMP_RS00570 0.27 -0.2 111,302 - MMP_RS00570 0.37 -0.0 111,700 - +0.1 111,786 - -0.2 111,789 - -0.5 112,635 - -4.1 112,669 - -0.0 112,734 - -0.3 112,734 - +0.5 112,751 - +0.3 112,752 + +0.7 112,777 - +0.5 112,798 - -0.4 112,798 - -0.2 112,799 + -0.7 112,799 + +0.0 112,809 - +0.8
Or see this region's nucleotide sequence