Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00500

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00495 and MMP_RS00500 overlap by 11 nucleotidesMMP_RS00500 and MMP_RS00505 are separated by 4 nucleotidesMMP_RS00505 and MMP_RS00510 are separated by 14 nucleotides MMP_RS00495: MMP_RS00495 - hydroxymethylglutaryl-CoA reductase (NADPH), at 97,761 to 98,987 _RS00495 MMP_RS00500: MMP_RS00500 - glutamyl-tRNA reductase, at 98,977 to 100,125 _RS00500 MMP_RS00505: MMP_RS00505 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 100,130 to 100,735 _RS00505 MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817 _RS00510 Position (kb) 98 99 100 101Strain fitness (log2 ratio) -3 -2 -1 0 1at 98.085 kb on + strandat 98.470 kb on + strandat 98.639 kb on - strandat 98.643 kb on + strandat 98.781 kb on + strandat 100.089 kb on - strandat 100.771 kb on + strandat 100.774 kb on - strandat 100.781 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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98,085 + -0.9
98,470 + -2.7
98,639 - -2.9
98,643 + -3.7
98,781 + -1.0
100,089 - -2.2
100,771 + -1.9
100,774 - -0.2
100,781 - -1.9

Or see this region's nucleotide sequence