Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00485 and MMP_RS00490 overlap by 1 nucleotides MMP_RS00490 and MMP_RS00495 are separated by 134 nucleotides MMP_RS00495 and MMP_RS00500 overlap by 11 nucleotides
MMP_RS00485: MMP_RS00485 - mRNA surveillance protein pelota, at 96,131 to 97,177
_RS00485
MMP_RS00490: MMP_RS00490 - Tfx family DNA-binding protein, at 97,177 to 97,626
_RS00490
MMP_RS00495: MMP_RS00495 - hydroxymethylglutaryl-CoA reductase (NADPH), at 97,761 to 98,987
_RS00495
MMP_RS00500: MMP_RS00500 - glutamyl-tRNA reductase, at 98,977 to 100,125
_RS00500
Position (kb)
97
98
99 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 96.940 kb on - strand at 97.091 kb on - strand at 97.092 kb on + strand at 97.279 kb on - strand at 97.351 kb on - strand at 97.357 kb on - strand at 97.401 kb on - strand at 97.406 kb on + strand at 97.419 kb on - strand at 97.424 kb on + strand at 97.435 kb on + strand at 97.457 kb on - strand at 97.458 kb on + strand at 97.461 kb on - strand at 97.530 kb on - strand at 97.546 kb on + strand at 97.626 kb on - strand at 97.654 kb on - strand at 97.654 kb on - strand at 97.655 kb on + strand at 97.655 kb on + strand at 97.656 kb on - strand at 97.656 kb on - strand at 97.656 kb on - strand at 97.677 kb on - strand at 97.678 kb on + strand at 97.682 kb on - strand at 98.085 kb on + strand at 98.470 kb on + strand at 98.639 kb on - strand at 98.643 kb on + strand at 98.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 96,940 - -3.9 97,091 - -1.2 97,092 + -0.2 97,279 - +0.2 97,351 - +0.1 97,357 - +1.8 97,401 - -0.0 97,406 + -3.0 97,419 - +2.4 97,424 + -1.7 97,435 + -0.5 97,457 - -0.4 97,458 + -0.0 97,461 - +1.9 97,530 - +3.0 97,546 + -1.2 97,626 - +4.4 97,654 - +0.3 97,654 - +0.1 97,655 + -1.5 97,655 + +0.2 97,656 - +1.4 97,656 - +2.0 97,656 - +0.1 97,677 - +2.1 97,678 + -0.8 97,682 - +0.1 98,085 + -0.9 98,470 + -2.7 98,639 - -2.9 98,643 + -3.7 98,781 + -1.0
Or see this region's nucleotide sequence