Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00460 and MMP_RS00465 are separated by 27 nucleotides MMP_RS00465 and MMP_RS00470 are separated by 20 nucleotides MMP_RS00470 and MMP_RS00475 are separated by 32 nucleotides
MMP_RS00460: MMP_RS00460 - glutamine amidotransferase family protein, at 90,509 to 91,600
_RS00460
MMP_RS00465: MMP_RS00465 - alpha-hydroxy-acid oxidizing protein, at 91,628 to 93,160
_RS00465
MMP_RS00470: MMP_RS00470 - hypothetical protein, at 93,181 to 93,951
_RS00470
MMP_RS00475: MMP_RS00475 - Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal domain, at 93,984 to 95,063
_RS00475
Position (kb) 91
92
93
94 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 91.203 kb on - strand at 91.703 kb on + strand at 91.888 kb on + strand at 91.953 kb on + strand at 92.001 kb on - strand at 92.002 kb on + strand at 92.782 kb on + strand at 93.954 kb on + strand at 93.973 kb on + strand at 93.973 kb on + strand at 94.078 kb on - strand at 94.080 kb on - strand at 94.130 kb on - strand, within MMP_RS00475 at 94.131 kb on + strand, within MMP_RS00475 at 94.134 kb on + strand, within MMP_RS00475
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 91,203 - -3.2 91,703 + -1.8 91,888 + -2.5 91,953 + +1.0 92,001 - +0.8 92,002 + -2.5 92,782 + -3.5 93,954 + -1.1 93,973 + -0.4 93,973 + -1.1 94,078 - +0.3 94,080 - -0.7 94,130 - MMP_RS00475 0.14 +0.9 94,131 + MMP_RS00475 0.14 +1.6 94,134 + MMP_RS00475 0.14 +0.2
Or see this region's nucleotide sequence