Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00420 and MMP_RS00425 are separated by 132 nucleotides MMP_RS00425 and MMP_RS00430 are separated by 69 nucleotides MMP_RS00430 and MMP_RS00435 overlap by 12 nucleotides
MMP_RS00420: MMP_RS00420 - (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 83,849 to 84,829
_RS00420
MMP_RS00425: MMP_RS00425 - argininosuccinate synthase, at 84,962 to 86,155
_RS00425
MMP_RS00430: MMP_RS00430 - hypothetical protein, at 86,225 to 86,581
_RS00430
MMP_RS00435: MMP_RS00435 - CPBP family intramembrane metalloprotease, at 86,570 to 87,379
_RS00435
Position (kb)
84
85
86
87 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 84.164 kb on - strand at 84.164 kb on - strand at 84.880 kb on - strand at 85.434 kb on + strand at 86.476 kb on - strand, within MMP_RS00430 at 86.476 kb on - strand, within MMP_RS00430 at 86.478 kb on - strand, within MMP_RS00430 at 86.610 kb on + strand at 86.673 kb on + strand, within MMP_RS00435 at 86.679 kb on + strand, within MMP_RS00435 at 86.684 kb on - strand, within MMP_RS00435 at 86.734 kb on - strand, within MMP_RS00435 at 86.737 kb on + strand, within MMP_RS00435 at 86.789 kb on - strand, within MMP_RS00435 at 86.790 kb on + strand, within MMP_RS00435 at 86.851 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.855 kb on - strand, within MMP_RS00435 at 86.856 kb on + strand, within MMP_RS00435 at 86.902 kb on - strand, within MMP_RS00435 at 86.902 kb on - strand, within MMP_RS00435 at 86.940 kb on - strand, within MMP_RS00435 at 86.968 kb on - strand, within MMP_RS00435 at 86.987 kb on - strand, within MMP_RS00435 at 87.110 kb on - strand, within MMP_RS00435 at 87.113 kb on + strand, within MMP_RS00435 at 87.134 kb on - strand, within MMP_RS00435
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 84,164 - -0.7 84,164 - -1.3 84,880 - -3.2 85,434 + +1.4 86,476 - MMP_RS00430 0.70 +0.4 86,476 - MMP_RS00430 0.70 +1.3 86,478 - MMP_RS00430 0.71 +0.1 86,610 + +0.8 86,673 + MMP_RS00435 0.13 -0.4 86,679 + MMP_RS00435 0.13 +0.1 86,684 - MMP_RS00435 0.14 +0.1 86,734 - MMP_RS00435 0.20 -0.1 86,737 + MMP_RS00435 0.21 +0.7 86,789 - MMP_RS00435 0.27 -0.2 86,790 + MMP_RS00435 0.27 +0.6 86,851 - MMP_RS00435 0.35 +0.3 86,855 - MMP_RS00435 0.35 -0.1 86,855 - MMP_RS00435 0.35 +0.9 86,855 - MMP_RS00435 0.35 +1.1 86,855 - MMP_RS00435 0.35 +0.1 86,856 + MMP_RS00435 0.35 -0.2 86,902 - MMP_RS00435 0.41 +1.7 86,902 - MMP_RS00435 0.41 +0.3 86,940 - MMP_RS00435 0.46 -1.6 86,968 - MMP_RS00435 0.49 +0.3 86,987 - MMP_RS00435 0.51 +0.8 87,110 - MMP_RS00435 0.67 +0.2 87,113 + MMP_RS00435 0.67 +0.8 87,134 - MMP_RS00435 0.70 +1.3
Or see this region's nucleotide sequence