Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00415

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00400 and MMP_RS00405 are separated by 44 nucleotidesMMP_RS00405 and MMP_RS00410 are separated by 259 nucleotidesMMP_RS00410 and MMP_RS00415 are separated by 67 nucleotidesMMP_RS00415 and MMP_RS00420 are separated by 114 nucleotides MMP_RS00400: MMP_RS00400 - tributyrin esterase, at 81,202 to 82,140 _RS00400 MMP_RS00405: MMP_RS00405 - tRNA-Thr, at 82,185 to 82,258 _RS00405 MMP_RS00410: MMP_RS00410 - tRNA-Gln, at 82,518 to 82,593 _RS00410 MMP_RS00415: MMP_RS00415 - DNA primase catalytic subunit PriS, at 82,661 to 83,734 _RS00415 MMP_RS00420: MMP_RS00420 - (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 83,849 to 84,829 _RS00420 Position (kb) 82 83 84Strain fitness (log2 ratio) -2 -1 0 1at 81.914 kb on - strand, within MMP_RS00400at 82.031 kb on + strand, within MMP_RS00400at 82.089 kb on - strandat 82.089 kb on - strandat 82.130 kb on - strandat 82.415 kb on - strandat 82.526 kb on + strandat 82.710 kb on - strandat 83.777 kb on - strandat 83.790 kb on - strandat 83.790 kb on - strandat 83.791 kb on + strandat 83.796 kb on - strandat 83.803 kb on + strandat 83.837 kb on - strandat 83.838 kb on + strandat 83.863 kb on + strandat 84.164 kb on - strandat 84.164 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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81,914 - MMP_RS00400 0.76 -0.2
82,031 + MMP_RS00400 0.88 -0.7
82,089 - +0.5
82,089 - +0.7
82,130 - +0.9
82,415 - -0.5
82,526 + -0.2
82,710 - -1.2
83,777 - -0.4
83,790 - +1.1
83,790 - -0.2
83,791 + +0.7
83,796 - +0.3
83,803 + -0.0
83,837 - +0.4
83,838 + -1.1
83,863 + -2.2
84,164 - -0.7
84,164 - -1.3

Or see this region's nucleotide sequence