Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00400 and MMP_RS00405 are separated by 44 nucleotides MMP_RS00405 and MMP_RS00410 are separated by 259 nucleotides MMP_RS00410 and MMP_RS00415 are separated by 67 nucleotides MMP_RS00415 and MMP_RS00420 are separated by 114 nucleotides
MMP_RS00400: MMP_RS00400 - tributyrin esterase, at 81,202 to 82,140
_RS00400
MMP_RS00405: MMP_RS00405 - tRNA-Thr, at 82,185 to 82,258
_RS00405
MMP_RS00410: MMP_RS00410 - tRNA-Gln, at 82,518 to 82,593
_RS00410
MMP_RS00415: MMP_RS00415 - DNA primase catalytic subunit PriS, at 82,661 to 83,734
_RS00415
MMP_RS00420: MMP_RS00420 - (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase, at 83,849 to 84,829
_RS00420
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-2
-1
0
1 at 81.914 kb on - strand, within MMP_RS00400 at 82.031 kb on + strand, within MMP_RS00400 at 82.089 kb on - strand at 82.089 kb on - strand at 82.130 kb on - strand at 82.415 kb on - strand at 82.526 kb on + strand at 82.710 kb on - strand at 83.777 kb on - strand at 83.790 kb on - strand at 83.790 kb on - strand at 83.791 kb on + strand at 83.796 kb on - strand at 83.803 kb on + strand at 83.837 kb on - strand at 83.838 kb on + strand at 83.863 kb on + strand at 84.164 kb on - strand at 84.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 81,914 - MMP_RS00400 0.76 -0.2 82,031 + MMP_RS00400 0.88 -0.7 82,089 - +0.5 82,089 - +0.7 82,130 - +0.9 82,415 - -0.5 82,526 + -0.2 82,710 - -1.2 83,777 - -0.4 83,790 - +1.1 83,790 - -0.2 83,791 + +0.7 83,796 - +0.3 83,803 + -0.0 83,837 - +0.4 83,838 + -1.1 83,863 + -2.2 84,164 - -0.7 84,164 - -1.3
Or see this region's nucleotide sequence