Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00340 and MMP_RS00345 are separated by 64 nucleotides MMP_RS00345 and MMP_RS00350 are separated by 108 nucleotides MMP_RS00350 and MMP_RS00355 are separated by 18 nucleotides MMP_RS00355 and MMP_RS00360 are separated by 36 nucleotides MMP_RS00360 and MMP_RS00365 are separated by 94 nucleotides
MMP_RS00340: MMP_RS00340 - 5 10-methylenetetrahydromethanopterin reductase, at 72,602 to 73,564
_RS00340
MMP_RS00345: MMP_RS00345 - DUF2666 domain-containing protein, at 73,629 to 74,051
_RS00345
MMP_RS00350: MMP_RS00350 - 50S ribosomal protein L18a, at 74,160 to 74,390
_RS00350
MMP_RS00355: MMP_RS00355 - translation initiation factor IF-6, at 74,409 to 75,092
_RS00355
MMP_RS00360: MMP_RS00360 - 50S ribosomal protein L31e, at 75,129 to 75,380
_RS00360
MMP_RS00365: MMP_RS00365 - acetylglutamate kinase, at 75,475 to 76,359
_RS00365
Position (kb)
74
75
76 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 73.533 kb on - strand at 73.617 kb on + strand at 73.690 kb on + strand, within MMP_RS00345 at 73.821 kb on + strand, within MMP_RS00345 at 73.832 kb on - strand, within MMP_RS00345 at 73.857 kb on - strand, within MMP_RS00345 at 73.917 kb on - strand, within MMP_RS00345 at 74.078 kb on - strand at 74.080 kb on - strand at 74.109 kb on + strand at 74.110 kb on - strand at 74.111 kb on + strand at 74.113 kb on + strand at 74.688 kb on - strand at 74.763 kb on - strand at 75.135 kb on - strand at 75.783 kb on + strand at 75.962 kb on - strand at 75.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 73,533 - -6.3 73,617 + +0.7 73,690 + MMP_RS00345 0.14 +0.5 73,821 + MMP_RS00345 0.45 -0.6 73,832 - MMP_RS00345 0.48 +1.6 73,857 - MMP_RS00345 0.54 +0.6 73,917 - MMP_RS00345 0.68 -0.5 74,078 - +0.1 74,080 - +0.1 74,109 + +0.9 74,110 - +0.2 74,111 + +0.3 74,113 + -0.0 74,688 - -3.6 74,763 - -1.7 75,135 - -1.5 75,783 + -4.3 75,962 - -0.8 75,968 - -3.4
Or see this region's nucleotide sequence