Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00250 and MMP_RS00255 are separated by 9 nucleotides MMP_RS00255 and MMP_RS00260 are separated by 18 nucleotides MMP_RS00260 and MMP_RS00265 are separated by 503 nucleotides
MMP_RS00250: MMP_RS00250 - Flp pilus assembly complex ATPase component TadA, at 56,946 to 58,595
_RS00250
MMP_RS00255: MMP_RS00255 - transcription initiation factor IIB, at 58,605 to 59,624
_RS00255
MMP_RS00260: MMP_RS00260 - Gar1/Naf1 family protein, at 59,643 to 59,924
_RS00260
MMP_RS00265: MMP_RS00265 - type 2 isopentenyl-diphosphate Delta-isomerase, at 60,428 to 61,495
_RS00265
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 57.606 kb on - strand, within MMP_RS00250 at 57.607 kb on + strand, within MMP_RS00250 at 57.621 kb on + strand, within MMP_RS00250 at 57.622 kb on - strand, within MMP_RS00250 at 57.623 kb on + strand, within MMP_RS00250 at 57.623 kb on + strand, within MMP_RS00250 at 57.657 kb on + strand, within MMP_RS00250 at 57.715 kb on - strand, within MMP_RS00250 at 57.813 kb on + strand, within MMP_RS00250 at 57.939 kb on - strand, within MMP_RS00250 at 57.941 kb on - strand, within MMP_RS00250 at 58.107 kb on - strand, within MMP_RS00250 at 58.111 kb on + strand, within MMP_RS00250 at 58.115 kb on + strand, within MMP_RS00250 at 58.121 kb on + strand, within MMP_RS00250 at 58.128 kb on - strand, within MMP_RS00250 at 58.128 kb on - strand, within MMP_RS00250 at 58.143 kb on - strand, within MMP_RS00250 at 58.143 kb on - strand, within MMP_RS00250 at 58.144 kb on + strand, within MMP_RS00250 at 58.220 kb on - strand, within MMP_RS00250 at 58.221 kb on + strand, within MMP_RS00250 at 58.243 kb on + strand, within MMP_RS00250 at 58.243 kb on + strand, within MMP_RS00250 at 58.253 kb on - strand, within MMP_RS00250 at 58.259 kb on - strand, within MMP_RS00250 at 58.313 kb on + strand, within MMP_RS00250 at 58.313 kb on + strand, within MMP_RS00250 at 58.313 kb on + strand, within MMP_RS00250 at 58.315 kb on + strand, within MMP_RS00250 at 58.344 kb on - strand, within MMP_RS00250 at 58.344 kb on - strand, within MMP_RS00250 at 58.378 kb on - strand, within MMP_RS00250 at 58.379 kb on + strand, within MMP_RS00250 at 58.379 kb on + strand, within MMP_RS00250 at 58.389 kb on - strand, within MMP_RS00250 at 58.401 kb on + strand, within MMP_RS00250 at 58.500 kb on - strand at 58.500 kb on - strand at 58.501 kb on + strand at 58.501 kb on + strand at 58.531 kb on + strand at 58.531 kb on + strand at 58.595 kb on - strand at 58.607 kb on + strand at 58.608 kb on - strand at 58.614 kb on - strand at 58.627 kb on + strand at 58.653 kb on + strand at 58.664 kb on - strand at 59.349 kb on + strand at 59.744 kb on - strand at 59.755 kb on + strand at 59.760 kb on - strand at 59.765 kb on + strand at 59.787 kb on - strand at 59.787 kb on - strand at 59.787 kb on - strand at 59.794 kb on - strand at 59.820 kb on + strand at 59.825 kb on - strand at 59.825 kb on - strand at 59.880 kb on - strand at 59.901 kb on + strand at 59.908 kb on + strand at 59.950 kb on + strand at 59.995 kb on - strand at 59.995 kb on - strand at 60.013 kb on - strand at 60.020 kb on - strand at 60.042 kb on - strand at 60.056 kb on - strand at 60.099 kb on + strand at 60.099 kb on + strand at 60.102 kb on - strand at 60.102 kb on - strand at 60.102 kb on - strand at 60.124 kb on + strand at 60.229 kb on - strand at 60.265 kb on + strand at 60.277 kb on - strand at 60.302 kb on + strand at 60.312 kb on - strand at 60.315 kb on - strand at 60.316 kb on + strand at 60.316 kb on + strand at 60.331 kb on + strand at 60.381 kb on + strand at 60.401 kb on - strand at 60.402 kb on + strand at 60.411 kb on - strand at 60.411 kb on - strand at 60.411 kb on - strand at 60.412 kb on + strand at 60.412 kb on + strand at 60.415 kb on - strand at 60.416 kb on + strand at 60.416 kb on + strand at 60.452 kb on + strand at 60.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 57,606 - MMP_RS00250 0.40 +0.1 57,607 + MMP_RS00250 0.40 +0.1 57,621 + MMP_RS00250 0.41 -0.2 57,622 - MMP_RS00250 0.41 +0.2 57,623 + MMP_RS00250 0.41 +0.4 57,623 + MMP_RS00250 0.41 +0.6 57,657 + MMP_RS00250 0.43 -1.1 57,715 - MMP_RS00250 0.47 -1.7 57,813 + MMP_RS00250 0.53 +0.6 57,939 - MMP_RS00250 0.60 -0.0 57,941 - MMP_RS00250 0.60 +0.1 58,107 - MMP_RS00250 0.70 -0.1 58,111 + MMP_RS00250 0.71 +0.1 58,115 + MMP_RS00250 0.71 -1.1 58,121 + MMP_RS00250 0.71 +0.5 58,128 - MMP_RS00250 0.72 +0.9 58,128 - MMP_RS00250 0.72 -0.5 58,143 - MMP_RS00250 0.73 +0.2 58,143 - MMP_RS00250 0.73 -0.4 58,144 + MMP_RS00250 0.73 -0.9 58,220 - MMP_RS00250 0.77 +0.5 58,221 + MMP_RS00250 0.77 +0.1 58,243 + MMP_RS00250 0.79 -0.6 58,243 + MMP_RS00250 0.79 +0.9 58,253 - MMP_RS00250 0.79 +0.6 58,259 - MMP_RS00250 0.80 -0.4 58,313 + MMP_RS00250 0.83 +0.4 58,313 + MMP_RS00250 0.83 -0.0 58,313 + MMP_RS00250 0.83 -0.1 58,315 + MMP_RS00250 0.83 -0.2 58,344 - MMP_RS00250 0.85 +0.4 58,344 - MMP_RS00250 0.85 +0.2 58,378 - MMP_RS00250 0.87 +0.2 58,379 + MMP_RS00250 0.87 +0.9 58,379 + MMP_RS00250 0.87 -1.0 58,389 - MMP_RS00250 0.87 +0.2 58,401 + MMP_RS00250 0.88 +0.5 58,500 - -0.3 58,500 - +0.1 58,501 + +1.2 58,501 + -0.5 58,531 + +0.3 58,531 + -0.3 58,595 - +0.1 58,607 + +0.6 58,608 - -0.6 58,614 - -0.5 58,627 + -3.2 58,653 + -0.0 58,664 - -0.9 59,349 + -4.2 59,744 - -3.2 59,755 + -4.0 59,760 - -3.6 59,765 + -3.3 59,787 - -5.2 59,787 - -4.8 59,787 - -4.6 59,794 - -2.6 59,820 + -1.7 59,825 - -3.7 59,825 - -2.7 59,880 - -2.7 59,901 + -3.2 59,908 + -4.9 59,950 + -4.7 59,995 - -3.7 59,995 - -4.9 60,013 - -4.8 60,020 - -1.3 60,042 - -0.2 60,056 - -1.4 60,099 + -0.7 60,099 + -1.1 60,102 - -0.2 60,102 - -0.3 60,102 - -0.8 60,124 + +0.6 60,229 - +0.5 60,265 + -0.0 60,277 - +0.2 60,302 + +1.0 60,312 - +0.4 60,315 - +0.8 60,316 + +0.1 60,316 + -1.1 60,331 + -0.1 60,381 + +0.1 60,401 - -1.7 60,402 + +0.4 60,411 - -0.2 60,411 - -1.2 60,411 - -2.6 60,412 + -3.7 60,412 + -0.2 60,415 - -2.2 60,416 + -3.2 60,416 + -2.7 60,452 + -0.5 60,470 + -2.6
Or see this region's nucleotide sequence