Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00225 and MMP_RS00230 are separated by 3 nucleotides MMP_RS00230 and MMP_RS00235 are separated by 10 nucleotides MMP_RS00235 and MMP_RS00240 are separated by 26 nucleotides
MMP_RS00225: MMP_RS00225 - ATP-grasp domain-containing protein, at 52,740 to 53,789
_RS00225
MMP_RS00230: MMP_RS00230 - transcription factor E, at 53,793 to 54,350
_RS00230
MMP_RS00235: MMP_RS00235 - HDIG domain-containing protein, at 54,361 to 54,948
_RS00235
MMP_RS00240: MMP_RS00240 - type II secretion system F family protein, at 54,975 to 55,871
_RS00240
Position (kb) 53
54
55 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 52.827 kb on - strand at 52.828 kb on + strand at 52.961 kb on - strand at 53.053 kb on - strand at 53.053 kb on - strand at 53.053 kb on - strand at 53.054 kb on + strand at 53.115 kb on - strand at 53.115 kb on - strand at 53.116 kb on + strand at 53.119 kb on - strand at 53.120 kb on + strand at 53.123 kb on + strand at 53.127 kb on + strand at 53.129 kb on - strand at 53.176 kb on - strand at 53.177 kb on + strand at 53.177 kb on + strand at 53.247 kb on + strand at 53.248 kb on - strand at 53.253 kb on - strand at 53.292 kb on - strand at 53.292 kb on - strand at 53.292 kb on - strand at 53.293 kb on + strand at 53.306 kb on - strand at 53.348 kb on - strand at 53.355 kb on + strand at 53.355 kb on + strand at 53.355 kb on + strand at 53.359 kb on + strand at 53.425 kb on - strand at 53.490 kb on - strand at 53.543 kb on + strand at 53.543 kb on + strand at 53.553 kb on - strand at 53.553 kb on - strand at 53.620 kb on - strand at 53.647 kb on + strand at 53.697 kb on - strand at 53.852 kb on + strand at 53.902 kb on - strand at 53.906 kb on - strand at 53.907 kb on + strand at 53.912 kb on - strand at 53.913 kb on + strand at 53.916 kb on + strand at 53.916 kb on + strand at 53.973 kb on + strand at 54.016 kb on + strand at 54.054 kb on - strand at 54.055 kb on + strand at 54.140 kb on + strand at 54.155 kb on - strand at 54.225 kb on + strand at 54.252 kb on - strand at 54.253 kb on + strand at 54.307 kb on - strand at 54.342 kb on + strand at 54.345 kb on - strand at 54.345 kb on - strand at 54.345 kb on - strand at 54.360 kb on - strand at 54.361 kb on + strand at 54.367 kb on + strand at 54.386 kb on - strand at 54.386 kb on - strand at 54.396 kb on - strand at 54.402 kb on + strand at 54.402 kb on + strand at 54.402 kb on + strand at 54.441 kb on + strand, within MMP_RS00235 at 54.444 kb on - strand, within MMP_RS00235 at 54.551 kb on - strand, within MMP_RS00235 at 54.575 kb on - strand, within MMP_RS00235 at 54.647 kb on - strand, within MMP_RS00235 at 54.662 kb on - strand, within MMP_RS00235 at 54.666 kb on + strand, within MMP_RS00235 at 54.668 kb on - strand, within MMP_RS00235 at 54.692 kb on + strand, within MMP_RS00235 at 54.732 kb on - strand, within MMP_RS00235 at 54.772 kb on - strand, within MMP_RS00235 at 54.773 kb on + strand, within MMP_RS00235 at 54.821 kb on - strand, within MMP_RS00235 at 54.907 kb on - strand at 54.920 kb on - strand at 54.930 kb on - strand at 54.930 kb on - strand at 54.930 kb on - strand at 54.931 kb on + strand at 54.936 kb on + strand at 54.947 kb on + strand at 54.947 kb on + strand at 54.977 kb on + strand at 54.977 kb on + strand at 54.984 kb on - strand at 54.996 kb on + strand at 55.005 kb on - strand at 55.005 kb on - strand at 55.041 kb on - strand at 55.042 kb on + strand at 55.052 kb on - strand at 55.057 kb on + strand at 55.065 kb on + strand, within MMP_RS00240 at 55.068 kb on - strand, within MMP_RS00240 at 55.150 kb on + strand, within MMP_RS00240 at 55.254 kb on - strand, within MMP_RS00240 at 55.290 kb on - strand, within MMP_RS00240
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 52,827 - -0.9 52,828 + -3.0 52,961 - -2.2 53,053 - -3.8 53,053 - -3.5 53,053 - -4.0 53,054 + -4.6 53,115 - -4.7 53,115 - -3.0 53,116 + -3.4 53,119 - -3.6 53,120 + -6.2 53,123 + -4.6 53,127 + -4.9 53,129 - -4.1 53,176 - -3.6 53,177 + -5.2 53,177 + -5.5 53,247 + -3.7 53,248 - -4.1 53,253 - -4.4 53,292 - -4.0 53,292 - -4.2 53,292 - -2.5 53,293 + -3.9 53,306 - -2.0 53,348 - -2.5 53,355 + -3.9 53,355 + -3.3 53,355 + -5.3 53,359 + -2.7 53,425 - -2.3 53,490 - -2.2 53,543 + -3.2 53,543 + -4.4 53,553 - -1.2 53,553 - -4.3 53,620 - -1.9 53,647 + -4.0 53,697 - -2.2 53,852 + -3.2 53,902 - -2.6 53,906 - -3.9 53,907 + -2.2 53,912 - -1.9 53,913 + -3.7 53,916 + -4.8 53,916 + -4.8 53,973 + -3.6 54,016 + -3.7 54,054 - -4.4 54,055 + -2.9 54,140 + -0.2 54,155 - -3.9 54,225 + -4.3 54,252 - -5.6 54,253 + -4.9 54,307 - -2.3 54,342 + +1.1 54,345 - +0.7 54,345 - +0.2 54,345 - -0.7 54,360 - +0.6 54,361 + -2.0 54,367 + +0.8 54,386 - +0.1 54,386 - -1.6 54,396 - -0.6 54,402 + +0.3 54,402 + +1.1 54,402 + +0.3 54,441 + MMP_RS00235 0.14 -0.2 54,444 - MMP_RS00235 0.14 +0.7 54,551 - MMP_RS00235 0.32 +0.0 54,575 - MMP_RS00235 0.36 +0.6 54,647 - MMP_RS00235 0.49 +0.7 54,662 - MMP_RS00235 0.51 +0.5 54,666 + MMP_RS00235 0.52 +0.9 54,668 - MMP_RS00235 0.52 +0.3 54,692 + MMP_RS00235 0.56 +0.2 54,732 - MMP_RS00235 0.63 -0.3 54,772 - MMP_RS00235 0.70 -0.4 54,773 + MMP_RS00235 0.70 -0.8 54,821 - MMP_RS00235 0.78 -0.0 54,907 - +0.6 54,920 - -0.1 54,930 - +0.9 54,930 - -0.0 54,930 - -0.7 54,931 + +0.1 54,936 + +0.1 54,947 + +0.4 54,947 + +0.4 54,977 + +1.5 54,977 + +1.1 54,984 - +0.4 54,996 + +0.4 55,005 - +1.5 55,005 - +0.4 55,041 - +0.4 55,042 + -0.6 55,052 - +0.4 55,057 + +0.7 55,065 + MMP_RS00240 0.10 +0.5 55,068 - MMP_RS00240 0.10 +0.6 55,150 + MMP_RS00240 0.20 +0.1 55,254 - MMP_RS00240 0.31 -0.0 55,290 - MMP_RS00240 0.35 -0.5
Or see this region's nucleotide sequence