Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00210 and MMP_RS00215 are separated by 610 nucleotides MMP_RS00215 and MMP_RS00220 are separated by 20 nucleotides MMP_RS00220 and MMP_RS00225 are separated by 25 nucleotides
MMP_RS00210: MMP_RS00210 - helix-turn-helix domain-containing protein, at 49,388 to 49,909
_RS00210
MMP_RS00215: MMP_RS00215 - AAA family ATPase, at 50,520 to 51,746
_RS00215
MMP_RS00220: MMP_RS00220 - GTP cyclohydrolase MptA, at 51,767 to 52,714
_RS00220
MMP_RS00225: MMP_RS00225 - ATP-grasp domain-containing protein, at 52,740 to 53,789
_RS00225
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 49.523 kb on + strand at 49.574 kb on - strand at 49.799 kb on - strand at 50.010 kb on + strand at 50.364 kb on - strand at 50.449 kb on + strand at 50.468 kb on + strand at 50.468 kb on + strand at 50.765 kb on + strand at 50.770 kb on + strand at 51.686 kb on + strand at 51.944 kb on + strand at 51.987 kb on - strand at 52.035 kb on - strand at 52.084 kb on + strand at 52.100 kb on - strand at 52.101 kb on + strand at 52.101 kb on + strand at 52.101 kb on + strand at 52.180 kb on + strand at 52.289 kb on + strand at 52.346 kb on + strand at 52.406 kb on - strand at 52.409 kb on + strand at 52.466 kb on - strand at 52.503 kb on - strand at 52.583 kb on - strand at 52.670 kb on + strand at 52.683 kb on - strand at 52.684 kb on + strand at 52.684 kb on + strand at 52.684 kb on + strand at 52.696 kb on - strand at 52.697 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 49,523 + -1.6 49,574 - -0.6 49,799 - -0.6 50,010 + -0.0 50,364 - +0.6 50,449 + -1.5 50,468 + -2.1 50,468 + -3.0 50,765 + -4.9 50,770 + -2.2 51,686 + -3.3 51,944 + -3.0 51,987 - -4.1 52,035 - -2.4 52,084 + -4.1 52,100 - -0.9 52,101 + -2.1 52,101 + -3.2 52,101 + -1.4 52,180 + -1.4 52,289 + -3.4 52,346 + -5.7 52,406 - -3.5 52,409 + -3.9 52,466 - -3.0 52,503 - -2.9 52,583 - -3.5 52,670 + -0.8 52,683 - -4.3 52,684 + -2.1 52,684 + -3.3 52,684 + -2.3 52,696 - -2.0 52,697 + -0.2
Or see this region's nucleotide sequence