Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00200 and MMP_RS00205 are separated by 15 nucleotides MMP_RS00205 and MMP_RS00210 are separated by 632 nucleotides
MMP_RS00200: MMP_RS00200 - minichromosome maintenance protein MCM, at 45,639 to 47,657
_RS00200
MMP_RS00205: MMP_RS00205 - helix-turn-helix domain-containing protein, at 47,673 to 48,755
_RS00205
MMP_RS00210: MMP_RS00210 - helix-turn-helix domain-containing protein, at 49,388 to 49,909
_RS00210
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 46.853 kb on - strand at 46.996 kb on - strand at 47.118 kb on + strand at 47.122 kb on - strand at 47.585 kb on + strand at 48.672 kb on + strand at 48.779 kb on - strand at 48.830 kb on + strand at 48.833 kb on - strand at 48.833 kb on - strand at 48.834 kb on + strand at 48.834 kb on + strand at 48.851 kb on - strand at 48.855 kb on + strand at 48.855 kb on + strand at 48.859 kb on - strand at 48.860 kb on + strand at 48.862 kb on - strand at 48.862 kb on - strand at 48.867 kb on - strand at 48.868 kb on + strand at 48.868 kb on + strand at 48.875 kb on + strand at 48.879 kb on + strand at 48.906 kb on - strand at 48.907 kb on + strand at 49.334 kb on + strand at 49.334 kb on + strand at 49.350 kb on + strand at 49.357 kb on - strand at 49.361 kb on - strand at 49.361 kb on - strand at 49.361 kb on - strand at 49.386 kb on - strand at 49.389 kb on - strand at 49.389 kb on - strand at 49.389 kb on - strand at 49.394 kb on + strand at 49.458 kb on + strand at 49.517 kb on - strand at 49.523 kb on + strand at 49.574 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 46,853 - +1.4 46,996 - -5.1 47,118 + -2.2 47,122 - -0.2 47,585 + -2.7 48,672 + -0.0 48,779 - +0.0 48,830 + +0.7 48,833 - +0.3 48,833 - +0.6 48,834 + -0.5 48,834 + -0.1 48,851 - +0.5 48,855 + +0.8 48,855 + -1.1 48,859 - -0.4 48,860 + +0.5 48,862 - -2.4 48,862 - -3.6 48,867 - +0.3 48,868 + -0.1 48,868 + -0.5 48,875 + +0.8 48,879 + +0.5 48,906 - +1.1 48,907 + +0.1 49,334 + +0.5 49,334 + -1.9 49,350 + +1.0 49,357 - -0.5 49,361 - +0.2 49,361 - +0.2 49,361 - +0.2 49,386 - -1.1 49,389 - +0.4 49,389 - +0.5 49,389 - -0.2 49,394 + -0.8 49,458 + -1.2 49,517 - -2.2 49,523 + -1.6 49,574 - -0.6
Or see this region's nucleotide sequence