Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00160 and MMP_RS00165 are separated by 467 nucleotides MMP_RS00165 and MMP_RS00170 are separated by 56 nucleotides MMP_RS00170 and MMP_RS00175 are separated by 100 nucleotides MMP_RS00175 and MMP_RS00180 are separated by 54 nucleotides
MMP_RS00160: MMP_RS00160 - DNA polymerase II large subunit, at 33,256 to 36,651
_RS00160
MMP_RS00165: MMP_RS00165 - 5S ribosomal RNA, at 37,119 to 37,233
_RS00165
MMP_RS00170: MMP_RS00170 - 23S ribosomal RNA, at 37,290 to 40,257
_RS00170
MMP_RS00175: MMP_RS00175 - tRNA-Ala, at 40,358 to 40,430
_RS00175
MMP_RS00180: MMP_RS00180 - 16S ribosomal RNA, at 40,485 to 41,951
_RS00180
Position (kb) 37
38
39
40
41 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 36.578 kb on - strand at 36.590 kb on + strand at 36.713 kb on - strand at 36.713 kb on - strand at 36.713 kb on - strand at 36.714 kb on + strand at 36.714 kb on + strand at 36.714 kb on + strand at 36.714 kb on + strand at 36.729 kb on + strand at 36.744 kb on + strand at 36.750 kb on - strand at 36.751 kb on + strand at 36.760 kb on - strand at 36.760 kb on - strand at 36.976 kb on - strand at 36.977 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 36,578 - -4.1 36,590 + -3.3 36,713 - +0.1 36,713 - +0.6 36,713 - -0.3 36,714 + -1.3 36,714 + +0.3 36,714 + +0.4 36,714 + +1.1 36,729 + -0.1 36,744 + +0.3 36,750 - +0.4 36,751 + +0.3 36,760 - +0.1 36,760 - +0.4 36,976 - -1.3 36,977 + -3.7
Or see this region's nucleotide sequence