Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00085 and MMP_RS00090 are separated by 69 nucleotides MMP_RS00090 and MMP_RS00095 are separated by 41 nucleotides MMP_RS00095 and MMP_RS00100 are separated by 13 nucleotides
MMP_RS00085: MMP_RS00085 - DNA cytosine methyltransferase, at 20,974 to 22,041
_RS00085
MMP_RS00090: MMP_RS00090 - CoA-binding protein, at 22,111 to 22,560
_RS00090
MMP_RS00095: MMP_RS00095 - argininosuccinate lyase, at 22,602 to 24,047
_RS00095
MMP_RS00100: MMP_RS00100 - tRNA pseudouridine(13) synthase TruD, at 24,061 to 25,230
_RS00100
Position (kb) 22
23
24
25 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 21.692 kb on + strand, within MMP_RS00085 at 21.713 kb on - strand, within MMP_RS00085 at 21.721 kb on + strand, within MMP_RS00085 at 21.743 kb on - strand, within MMP_RS00085 at 21.763 kb on - strand, within MMP_RS00085 at 21.779 kb on + strand, within MMP_RS00085 at 21.826 kb on - strand, within MMP_RS00085 at 21.826 kb on - strand, within MMP_RS00085 at 21.827 kb on + strand, within MMP_RS00085 at 21.827 kb on + strand, within MMP_RS00085 at 22.073 kb on - strand at 22.073 kb on - strand at 22.074 kb on + strand at 22.075 kb on - strand at 22.075 kb on - strand at 22.075 kb on - strand at 22.076 kb on + strand at 22.076 kb on + strand at 22.076 kb on + strand at 22.076 kb on + strand at 22.076 kb on + strand at 22.089 kb on - strand at 22.109 kb on + strand at 22.109 kb on + strand at 22.128 kb on - strand at 22.128 kb on - strand at 22.128 kb on - strand at 22.158 kb on + strand, within MMP_RS00090 at 22.259 kb on + strand, within MMP_RS00090 at 22.279 kb on + strand, within MMP_RS00090 at 22.279 kb on + strand, within MMP_RS00090 at 22.334 kb on - strand, within MMP_RS00090 at 22.335 kb on + strand, within MMP_RS00090 at 22.335 kb on + strand, within MMP_RS00090 at 22.337 kb on + strand, within MMP_RS00090 at 22.540 kb on + strand at 22.576 kb on + strand at 23.139 kb on + strand at 23.160 kb on - strand at 23.160 kb on - strand at 23.696 kb on - strand at 24.040 kb on - strand at 24.045 kb on - strand at 24.091 kb on - strand at 24.222 kb on + strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.425 kb on - strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.426 kb on + strand, within MMP_RS00100 at 24.427 kb on - strand, within MMP_RS00100 at 24.428 kb on + strand, within MMP_RS00100 at 24.458 kb on + strand, within MMP_RS00100 at 24.488 kb on - strand, within MMP_RS00100 at 24.489 kb on + strand, within MMP_RS00100 at 24.529 kb on + strand, within MMP_RS00100 at 24.532 kb on - strand, within MMP_RS00100 at 24.533 kb on + strand, within MMP_RS00100 at 24.580 kb on - strand, within MMP_RS00100 at 24.581 kb on + strand, within MMP_RS00100 at 24.600 kb on - strand, within MMP_RS00100 at 24.608 kb on + strand, within MMP_RS00100 at 24.626 kb on - strand, within MMP_RS00100 at 24.973 kb on + strand, within MMP_RS00100
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 21,692 + MMP_RS00085 0.67 +0.4 21,713 - MMP_RS00085 0.69 +1.0 21,721 + MMP_RS00085 0.70 +0.3 21,743 - MMP_RS00085 0.72 +0.2 21,763 - MMP_RS00085 0.74 -0.4 21,779 + MMP_RS00085 0.75 +0.6 21,826 - MMP_RS00085 0.80 -0.9 21,826 - MMP_RS00085 0.80 +0.8 21,827 + MMP_RS00085 0.80 +0.9 21,827 + MMP_RS00085 0.80 +1.4 22,073 - -0.4 22,073 - -0.5 22,074 + -0.0 22,075 - -0.1 22,075 - +0.6 22,075 - +0.4 22,076 + +0.8 22,076 + +0.1 22,076 + +1.1 22,076 + -0.3 22,076 + -0.1 22,089 - +0.3 22,109 + +1.2 22,109 + +0.8 22,128 - -0.0 22,128 - -0.5 22,128 - +0.2 22,158 + MMP_RS00090 0.10 +0.7 22,259 + MMP_RS00090 0.33 -0.1 22,279 + MMP_RS00090 0.37 -0.3 22,279 + MMP_RS00090 0.37 -0.6 22,334 - MMP_RS00090 0.50 +0.4 22,335 + MMP_RS00090 0.50 +1.2 22,335 + MMP_RS00090 0.50 -0.3 22,337 + MMP_RS00090 0.50 -1.1 22,540 + +0.8 22,576 + +0.8 23,139 + -2.3 23,160 - -4.1 23,160 - -2.4 23,696 - -1.3 24,040 - -0.2 24,045 - +0.8 24,091 - +0.0 24,222 + MMP_RS00100 0.14 +0.8 24,425 - MMP_RS00100 0.31 -0.6 24,425 - MMP_RS00100 0.31 -0.5 24,425 - MMP_RS00100 0.31 +0.3 24,425 - MMP_RS00100 0.31 +0.3 24,426 + MMP_RS00100 0.31 -0.4 24,426 + MMP_RS00100 0.31 +0.1 24,426 + MMP_RS00100 0.31 -0.7 24,426 + MMP_RS00100 0.31 -0.6 24,426 + MMP_RS00100 0.31 +0.8 24,426 + MMP_RS00100 0.31 -0.2 24,427 - MMP_RS00100 0.31 +0.9 24,428 + MMP_RS00100 0.31 -0.7 24,458 + MMP_RS00100 0.34 +1.0 24,488 - MMP_RS00100 0.36 -0.9 24,489 + MMP_RS00100 0.37 -0.5 24,529 + MMP_RS00100 0.40 -0.4 24,532 - MMP_RS00100 0.40 +0.8 24,533 + MMP_RS00100 0.40 +1.0 24,580 - MMP_RS00100 0.44 -0.4 24,581 + MMP_RS00100 0.44 +0.0 24,600 - MMP_RS00100 0.46 -0.9 24,608 + MMP_RS00100 0.47 +0.4 24,626 - MMP_RS00100 0.48 -0.6 24,973 + MMP_RS00100 0.78 -0.4
Or see this region's nucleotide sequence