Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00070 and MMP_RS00075 are separated by 10 nucleotides MMP_RS00075 and MMP_RS00080 are separated by 26 nucleotides MMP_RS00080 and MMP_RS00085 are separated by 42 nucleotides
MMP_RS00070: MMP_RS00070 - DNA-directed DNA polymerase II small subunit, at 17,602 to 19,359
_RS00070
MMP_RS00075: MMP_RS00075 - hypothetical protein, at 19,370 to 20,479
_RS00075
MMP_RS00080: MMP_RS00080 - RecB-family nuclease, at 20,506 to 20,931
_RS00080
MMP_RS00085: MMP_RS00085 - DNA cytosine methyltransferase, at 20,974 to 22,041
_RS00085
Position (kb) 19
20
21 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 18.742 kb on - strand at 19.130 kb on - strand at 19.527 kb on + strand at 19.562 kb on - strand at 19.563 kb on + strand at 19.563 kb on + strand at 19.859 kb on + strand at 20.020 kb on + strand at 20.084 kb on - strand at 20.135 kb on - strand at 20.705 kb on + strand, within MMP_RS00080 at 20.707 kb on - strand, within MMP_RS00080 at 20.731 kb on - strand, within MMP_RS00080 at 20.839 kb on + strand, within MMP_RS00080 at 20.842 kb on - strand, within MMP_RS00080 at 20.961 kb on + strand at 20.975 kb on - strand at 20.976 kb on + strand at 20.979 kb on - strand at 20.979 kb on - strand at 20.980 kb on + strand at 21.054 kb on + strand at 21.128 kb on + strand, within MMP_RS00085 at 21.209 kb on - strand, within MMP_RS00085 at 21.271 kb on + strand, within MMP_RS00085
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 18,742 - -1.1 19,130 - -0.2 19,527 + -2.0 19,562 - -2.0 19,563 + -2.0 19,563 + -3.9 19,859 + -1.7 20,020 + -2.3 20,084 - +0.9 20,135 - -1.4 20,705 + MMP_RS00080 0.47 -0.7 20,707 - MMP_RS00080 0.47 -0.5 20,731 - MMP_RS00080 0.53 +0.7 20,839 + MMP_RS00080 0.78 +0.7 20,842 - MMP_RS00080 0.79 -0.5 20,961 + -0.2 20,975 - +0.6 20,976 + +0.3 20,979 - +0.1 20,979 - +0.5 20,980 + -0.2 21,054 + +0.7 21,128 + MMP_RS00085 0.14 -0.0 21,209 - MMP_RS00085 0.22 +0.5 21,271 + MMP_RS00085 0.28 -1.1
Or see this region's nucleotide sequence