Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00065 and MMP_RS00070 are separated by 28 nucleotides MMP_RS00070 and MMP_RS00075 are separated by 10 nucleotides
MMP_RS00065: MMP_RS00065 - geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase, at 16,803 to 17,573
_RS00065
MMP_RS00070: MMP_RS00070 - DNA-directed DNA polymerase II small subunit, at 17,602 to 19,359
_RS00070
MMP_RS00075: MMP_RS00075 - hypothetical protein, at 19,370 to 20,479
_RS00075
Position (kb)
17
18
19
20 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 16.605 kb on + strand at 16.628 kb on + strand at 16.647 kb on + strand at 16.648 kb on - strand at 16.655 kb on + strand at 16.655 kb on + strand at 16.682 kb on - strand at 16.683 kb on + strand at 16.683 kb on + strand at 16.737 kb on + strand at 16.782 kb on + strand at 16.791 kb on + strand at 16.883 kb on + strand at 17.295 kb on - strand at 17.552 kb on + strand at 17.596 kb on - strand at 17.956 kb on - strand at 18.316 kb on - strand at 18.362 kb on + strand at 18.742 kb on - strand at 19.130 kb on - strand at 19.527 kb on + strand at 19.562 kb on - strand at 19.563 kb on + strand at 19.563 kb on + strand at 19.859 kb on + strand at 20.020 kb on + strand at 20.084 kb on - strand at 20.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 16,605 + +0.8 16,628 + +0.4 16,647 + +0.4 16,648 - -0.2 16,655 + +0.3 16,655 + -0.5 16,682 - +0.6 16,683 + +0.2 16,683 + +1.3 16,737 + +0.2 16,782 + -3.0 16,791 + -2.1 16,883 + -1.4 17,295 - -3.7 17,552 + +0.3 17,596 - -0.3 17,956 - -0.6 18,316 - -1.2 18,362 + -2.2 18,742 - -1.1 19,130 - -0.2 19,527 + -2.0 19,562 - -2.0 19,563 + -2.0 19,563 + -3.9 19,859 + -1.7 20,020 + -2.3 20,084 - +0.9 20,135 - -1.4
Or see this region's nucleotide sequence