Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00060 and MMP_RS00065 are separated by 381 nucleotides MMP_RS00065 and MMP_RS00070 are separated by 28 nucleotides
MMP_RS00060: MMP_RS00060 - 3-dehydroquinate synthase II, at 15,336 to 16,421
_RS00060
MMP_RS00065: MMP_RS00065 - geranylgeranylglyceryl/heptaprenylglyceryl phosphate synthase, at 16,803 to 17,573
_RS00065
MMP_RS00070: MMP_RS00070 - DNA-directed DNA polymerase II small subunit, at 17,602 to 19,359
_RS00070
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 16.082 kb on + strand, within MMP_RS00060 at 16.458 kb on + strand at 16.524 kb on - strand at 16.525 kb on + strand at 16.525 kb on + strand at 16.525 kb on + strand at 16.536 kb on - strand at 16.536 kb on - strand at 16.536 kb on - strand at 16.537 kb on + strand at 16.555 kb on - strand at 16.556 kb on + strand at 16.556 kb on + strand at 16.556 kb on + strand at 16.569 kb on - strand at 16.578 kb on - strand at 16.579 kb on + strand at 16.605 kb on + strand at 16.628 kb on + strand at 16.647 kb on + strand at 16.648 kb on - strand at 16.655 kb on + strand at 16.655 kb on + strand at 16.682 kb on - strand at 16.683 kb on + strand at 16.683 kb on + strand at 16.737 kb on + strand at 16.782 kb on + strand at 16.791 kb on + strand at 16.883 kb on + strand at 17.295 kb on - strand at 17.552 kb on + strand at 17.596 kb on - strand at 17.956 kb on - strand at 18.316 kb on - strand at 18.362 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 16,082 + MMP_RS00060 0.69 -2.2 16,458 + +0.2 16,524 - +0.4 16,525 + +0.5 16,525 + -1.1 16,525 + +1.3 16,536 - -0.7 16,536 - +0.6 16,536 - +0.3 16,537 + +0.6 16,555 - -0.3 16,556 + +1.0 16,556 + +0.9 16,556 + +0.2 16,569 - -0.1 16,578 - +0.5 16,579 + +0.0 16,605 + +0.8 16,628 + +0.4 16,647 + +0.4 16,648 - -0.2 16,655 + +0.3 16,655 + -0.5 16,682 - +0.6 16,683 + +0.2 16,683 + +1.3 16,737 + +0.2 16,782 + -3.0 16,791 + -2.1 16,883 + -1.4 17,295 - -3.7 17,552 + +0.3 17,596 - -0.3 17,956 - -0.6 18,316 - -1.2 18,362 + -2.2
Or see this region's nucleotide sequence