Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS17535 and HSERO_RS17540 are separated by 58 nucleotides HSERO_RS17540 and HSERO_RS17545 are separated by 44 nucleotides HSERO_RS17545 and HSERO_RS17550 are separated by 30 nucleotides
HSERO_RS17535: HSERO_RS17535 - hypothetical protein, at 4,021,863 to 4,022,180
_RS17535
HSERO_RS17540: HSERO_RS17540 - glutamine ABC transporter ATP-binding protein, at 4,022,239 to 4,022,961
_RS17540
HSERO_RS17545: HSERO_RS17545 - glutamine ABC transporter permease, at 4,023,006 to 4,023,662
_RS17545
HSERO_RS17550: HSERO_RS17550 - amino acid ABC transporter substrate-binding protein, at 4,023,693 to 4,024,442
_RS17550
Position (kb)
4023
4024 Strain fitness (log2 ratio)
-2
-1
0
1 at 4022.012 kb on - strand, within HSERO_RS17535 at 4022.024 kb on - strand, within HSERO_RS17535 at 4022.166 kb on + strand at 4022.268 kb on + strand at 4022.268 kb on + strand at 4022.268 kb on + strand at 4022.276 kb on - strand at 4022.276 kb on - strand at 4022.276 kb on - strand at 4022.276 kb on - strand at 4022.276 kb on - strand at 4022.276 kb on - strand at 4022.341 kb on + strand, within HSERO_RS17540 at 4022.350 kb on + strand, within HSERO_RS17540 at 4022.351 kb on + strand, within HSERO_RS17540 at 4022.359 kb on - strand, within HSERO_RS17540 at 4022.360 kb on - strand, within HSERO_RS17540 at 4022.360 kb on - strand, within HSERO_RS17540 at 4022.406 kb on - strand, within HSERO_RS17540 at 4022.447 kb on - strand, within HSERO_RS17540 at 4022.496 kb on - strand, within HSERO_RS17540 at 4022.500 kb on - strand, within HSERO_RS17540 at 4022.599 kb on - strand, within HSERO_RS17540 at 4022.619 kb on - strand, within HSERO_RS17540 at 4022.619 kb on - strand, within HSERO_RS17540 at 4022.623 kb on + strand, within HSERO_RS17540 at 4022.754 kb on + strand, within HSERO_RS17540 at 4022.756 kb on - strand, within HSERO_RS17540 at 4022.914 kb on - strand at 4022.916 kb on - strand at 4023.050 kb on + strand at 4023.071 kb on + strand at 4023.071 kb on + strand at 4023.071 kb on + strand at 4023.079 kb on - strand, within HSERO_RS17545 at 4023.079 kb on - strand, within HSERO_RS17545 at 4023.079 kb on - strand, within HSERO_RS17545 at 4023.130 kb on + strand, within HSERO_RS17545 at 4023.137 kb on - strand, within HSERO_RS17545 at 4023.282 kb on + strand, within HSERO_RS17545 at 4023.290 kb on - strand at 4023.290 kb on - strand, within HSERO_RS17545 at 4023.293 kb on + strand, within HSERO_RS17545 at 4023.322 kb on + strand, within HSERO_RS17545 at 4023.374 kb on + strand, within HSERO_RS17545 at 4023.374 kb on + strand, within HSERO_RS17545 at 4023.376 kb on + strand, within HSERO_RS17545 at 4023.489 kb on - strand, within HSERO_RS17545 at 4023.618 kb on - strand at 4023.691 kb on + strand at 4023.699 kb on - strand at 4023.754 kb on + strand at 4023.754 kb on + strand at 4023.775 kb on + strand, within HSERO_RS17550 at 4023.783 kb on - strand, within HSERO_RS17550 at 4023.896 kb on - strand, within HSERO_RS17550 at 4023.932 kb on - strand, within HSERO_RS17550 at 4024.037 kb on - strand, within HSERO_RS17550 at 4024.172 kb on - strand, within HSERO_RS17550 at 4024.197 kb on + strand, within HSERO_RS17550 at 4024.363 kb on - strand, within HSERO_RS17550 at 4024.457 kb on - strand at 4024.479 kb on + strand at 4024.481 kb on + strand at 4024.583 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 4,022,012 - HSERO_RS17535 0.47 -0.3 4,022,024 - HSERO_RS17535 0.51 +1.4 4,022,166 + -1.8 4,022,268 + -0.5 4,022,268 + +0.1 4,022,268 + +0.3 4,022,276 - +0.0 4,022,276 - -0.5 4,022,276 - +0.8 4,022,276 - -0.5 4,022,276 - -0.9 4,022,276 - -0.2 4,022,341 + HSERO_RS17540 0.14 +0.4 4,022,350 + HSERO_RS17540 0.15 -0.5 4,022,351 + HSERO_RS17540 0.15 -0.2 4,022,359 - HSERO_RS17540 0.17 -0.8 4,022,360 - HSERO_RS17540 0.17 +1.2 4,022,360 - HSERO_RS17540 0.17 -0.9 4,022,406 - HSERO_RS17540 0.23 -0.6 4,022,447 - HSERO_RS17540 0.29 +0.1 4,022,496 - HSERO_RS17540 0.36 -0.1 4,022,500 - HSERO_RS17540 0.36 -1.0 4,022,599 - HSERO_RS17540 0.50 +0.7 4,022,619 - HSERO_RS17540 0.53 -0.3 4,022,619 - HSERO_RS17540 0.53 -0.2 4,022,623 + HSERO_RS17540 0.53 -0.3 4,022,754 + HSERO_RS17540 0.71 -0.9 4,022,756 - HSERO_RS17540 0.72 -1.5 4,022,914 - -0.1 4,022,916 - +1.1 4,023,050 + -0.3 4,023,071 + -0.3 4,023,071 + +0.4 4,023,071 + +0.3 4,023,079 - HSERO_RS17545 0.11 -0.1 4,023,079 - HSERO_RS17545 0.11 +0.1 4,023,079 - HSERO_RS17545 0.11 -0.3 4,023,130 + HSERO_RS17545 0.19 +1.5 4,023,137 - HSERO_RS17545 0.20 -0.5 4,023,282 + HSERO_RS17545 0.42 -0.0 4,023,290 - -1.1 4,023,290 - HSERO_RS17545 0.43 -1.0 4,023,293 + HSERO_RS17545 0.44 -0.2 4,023,322 + HSERO_RS17545 0.48 -0.6 4,023,374 + HSERO_RS17545 0.56 +0.2 4,023,374 + HSERO_RS17545 0.56 -1.2 4,023,376 + HSERO_RS17545 0.56 -0.3 4,023,489 - HSERO_RS17545 0.74 -1.0 4,023,618 - -0.3 4,023,691 + +0.2 4,023,699 - +0.4 4,023,754 + -0.4 4,023,754 + +0.2 4,023,775 + HSERO_RS17550 0.11 -0.2 4,023,783 - HSERO_RS17550 0.12 -0.4 4,023,896 - HSERO_RS17550 0.27 +1.6 4,023,932 - HSERO_RS17550 0.32 -0.5 4,024,037 - HSERO_RS17550 0.46 +1.0 4,024,172 - HSERO_RS17550 0.64 -0.1 4,024,197 + HSERO_RS17550 0.67 -1.4 4,024,363 - HSERO_RS17550 0.89 +0.9 4,024,457 - -0.1 4,024,479 + +0.4 4,024,481 + -1.4 4,024,583 + -2.3
Or see this region's nucleotide sequence