Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03200 and HSERO_RS03205 are separated by 93 nucleotides HSERO_RS03205 and HSERO_RS03210 are separated by 10 nucleotides HSERO_RS03210 and HSERO_RS03215 are separated by 20 nucleotides
HSERO_RS03200: HSERO_RS03200 - IMP dehydrogenase, at 700,133 to 703,519
_RS03200
HSERO_RS03205: HSERO_RS03205 - hypothetical protein, at 703,613 to 706,114
_RS03205
HSERO_RS03210: HSERO_RS03210 - transglutaminase, at 706,125 to 707,060
_RS03210
HSERO_RS03215: HSERO_RS03215 - sodium:solute symporter, at 707,081 to 709,132
_RS03215
Position (kb) 703
704
705
706
707 Strain fitness (log2 ratio) -2
-1
0
1 at 702.720 kb on + strand, within HSERO_RS03200 at 702.720 kb on + strand, within HSERO_RS03200 at 702.720 kb on + strand, within HSERO_RS03200 at 702.735 kb on + strand, within HSERO_RS03200 at 702.743 kb on - strand, within HSERO_RS03200 at 702.789 kb on - strand at 703.034 kb on + strand, within HSERO_RS03200 at 703.047 kb on + strand, within HSERO_RS03200 at 703.263 kb on + strand at 703.290 kb on + strand at 703.298 kb on - strand at 703.510 kb on - strand at 703.560 kb on + strand at 703.579 kb on - strand at 703.709 kb on - strand at 703.812 kb on - strand at 703.824 kb on - strand at 703.859 kb on + strand at 703.877 kb on - strand, within HSERO_RS03205 at 704.100 kb on + strand, within HSERO_RS03205 at 704.200 kb on - strand, within HSERO_RS03205 at 704.336 kb on - strand, within HSERO_RS03205 at 704.512 kb on + strand at 704.817 kb on - strand, within HSERO_RS03205 at 704.844 kb on - strand, within HSERO_RS03205 at 705.034 kb on + strand, within HSERO_RS03205 at 705.086 kb on - strand, within HSERO_RS03205 at 705.112 kb on - strand, within HSERO_RS03205 at 705.164 kb on + strand at 705.192 kb on + strand, within HSERO_RS03205 at 705.385 kb on + strand, within HSERO_RS03205 at 705.514 kb on - strand, within HSERO_RS03205 at 705.732 kb on - strand, within HSERO_RS03205 at 705.800 kb on + strand, within HSERO_RS03205 at 706.108 kb on + strand at 706.125 kb on - strand at 706.139 kb on + strand at 706.203 kb on + strand at 706.428 kb on - strand, within HSERO_RS03210 at 706.431 kb on + strand, within HSERO_RS03210 at 706.583 kb on - strand, within HSERO_RS03210 at 706.687 kb on + strand, within HSERO_RS03210 at 706.762 kb on + strand, within HSERO_RS03210 at 706.843 kb on + strand, within HSERO_RS03210 at 706.913 kb on - strand, within HSERO_RS03210
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 702,720 + HSERO_RS03200 0.76 +0.1 702,720 + HSERO_RS03200 0.76 -0.2 702,720 + HSERO_RS03200 0.76 +1.0 702,735 + HSERO_RS03200 0.77 +0.1 702,743 - HSERO_RS03200 0.77 -2.5 702,789 - -1.0 703,034 + HSERO_RS03200 0.86 +0.4 703,047 + HSERO_RS03200 0.86 +0.1 703,263 + +0.5 703,290 + +0.2 703,298 - +1.7 703,510 - +1.0 703,560 + +0.9 703,579 - -0.1 703,709 - -0.9 703,812 - +0.3 703,824 - -0.4 703,859 + +1.4 703,877 - HSERO_RS03205 0.11 +0.5 704,100 + HSERO_RS03205 0.19 -0.1 704,200 - HSERO_RS03205 0.23 -0.1 704,336 - HSERO_RS03205 0.29 +0.1 704,512 + -0.2 704,817 - HSERO_RS03205 0.48 -0.4 704,844 - HSERO_RS03205 0.49 -0.3 705,034 + HSERO_RS03205 0.57 -0.3 705,086 - HSERO_RS03205 0.59 -0.2 705,112 - HSERO_RS03205 0.60 +0.4 705,164 + +1.5 705,192 + HSERO_RS03205 0.63 +0.0 705,385 + HSERO_RS03205 0.71 +1.3 705,514 - HSERO_RS03205 0.76 -1.1 705,732 - HSERO_RS03205 0.85 -0.4 705,800 + HSERO_RS03205 0.87 -0.1 706,108 + +0.1 706,125 - -0.9 706,139 + +0.8 706,203 + +0.2 706,428 - HSERO_RS03210 0.32 -1.0 706,431 + HSERO_RS03210 0.33 -0.2 706,583 - HSERO_RS03210 0.49 -0.8 706,687 + HSERO_RS03210 0.60 +0.1 706,762 + HSERO_RS03210 0.68 -2.0 706,843 + HSERO_RS03210 0.77 -0.3 706,913 - HSERO_RS03210 0.84 +0.0
Or see this region's nucleotide sequence