Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00900 and HSERO_RS00905 are separated by 257 nucleotides HSERO_RS00905 and HSERO_RS00910 are separated by 198 nucleotides HSERO_RS00910 and HSERO_RS00915 are separated by 64 nucleotides
HSERO_RS00900: HSERO_RS00900 - L-proline ABC transporter, ATPase component 2 <small>(from data)</small>, at 187,220 to 187,945
_RS00900
HSERO_RS00905: HSERO_RS00905 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) <small>(from data)</small>, at 188,203 to 191,895
_RS00905
HSERO_RS00910: HSERO_RS00910 - peptidase, at 192,094 to 192,630
_RS00910
HSERO_RS00915: HSERO_RS00915 - ATP-dependent protease, at 192,695 to 194,041
_RS00915
Position (kb) 188
189
190
191
192 Strain fitness (log2 ratio) -2
-1
0
1 at 187.682 kb on + strand, within HSERO_RS00900 at 188.009 kb on + strand at 188.045 kb on - strand at 188.056 kb on + strand at 188.193 kb on + strand at 188.334 kb on - strand at 188.740 kb on + strand, within HSERO_RS00905 at 188.741 kb on + strand, within HSERO_RS00905 at 188.748 kb on - strand, within HSERO_RS00905 at 188.759 kb on - strand, within HSERO_RS00905 at 189.056 kb on + strand, within HSERO_RS00905 at 189.208 kb on + strand, within HSERO_RS00905 at 189.339 kb on + strand, within HSERO_RS00905 at 189.347 kb on - strand, within HSERO_RS00905 at 189.348 kb on + strand, within HSERO_RS00905 at 189.628 kb on - strand, within HSERO_RS00905 at 189.633 kb on - strand at 189.749 kb on + strand, within HSERO_RS00905 at 189.843 kb on + strand, within HSERO_RS00905 at 189.843 kb on + strand, within HSERO_RS00905 at 189.885 kb on - strand, within HSERO_RS00905 at 189.994 kb on + strand, within HSERO_RS00905 at 190.002 kb on - strand, within HSERO_RS00905 at 190.275 kb on - strand, within HSERO_RS00905 at 190.275 kb on - strand, within HSERO_RS00905 at 190.499 kb on + strand, within HSERO_RS00905 at 190.505 kb on - strand, within HSERO_RS00905 at 190.576 kb on - strand, within HSERO_RS00905 at 190.724 kb on + strand, within HSERO_RS00905 at 191.116 kb on - strand, within HSERO_RS00905 at 191.363 kb on - strand, within HSERO_RS00905 at 191.365 kb on + strand, within HSERO_RS00905 at 191.410 kb on - strand, within HSERO_RS00905 at 191.618 kb on - strand at 191.844 kb on - strand at 191.862 kb on + strand at 192.153 kb on + strand, within HSERO_RS00910 at 192.293 kb on + strand, within HSERO_RS00910 at 192.381 kb on + strand, within HSERO_RS00910 at 192.404 kb on - strand, within HSERO_RS00910 at 192.537 kb on + strand, within HSERO_RS00910 at 192.588 kb on + strand at 192.624 kb on + strand at 192.882 kb on - strand, within HSERO_RS00915
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 187,682 + HSERO_RS00900 0.64 +0.3 188,009 + -0.3 188,045 - -0.0 188,056 + -0.4 188,193 + -0.0 188,334 - -1.0 188,740 + HSERO_RS00905 0.15 -0.4 188,741 + HSERO_RS00905 0.15 -0.3 188,748 - HSERO_RS00905 0.15 +0.1 188,759 - HSERO_RS00905 0.15 -0.5 189,056 + HSERO_RS00905 0.23 -0.9 189,208 + HSERO_RS00905 0.27 -0.2 189,339 + HSERO_RS00905 0.31 -1.1 189,347 - HSERO_RS00905 0.31 -1.1 189,348 + HSERO_RS00905 0.31 -0.8 189,628 - HSERO_RS00905 0.39 -0.7 189,633 - +0.7 189,749 + HSERO_RS00905 0.42 -0.7 189,843 + HSERO_RS00905 0.44 -1.2 189,843 + HSERO_RS00905 0.44 -0.9 189,885 - HSERO_RS00905 0.46 -0.2 189,994 + HSERO_RS00905 0.48 -2.1 190,002 - HSERO_RS00905 0.49 -0.6 190,275 - HSERO_RS00905 0.56 +0.2 190,275 - HSERO_RS00905 0.56 -2.6 190,499 + HSERO_RS00905 0.62 +1.5 190,505 - HSERO_RS00905 0.62 -2.1 190,576 - HSERO_RS00905 0.64 -1.4 190,724 + HSERO_RS00905 0.68 -1.2 191,116 - HSERO_RS00905 0.79 -0.2 191,363 - HSERO_RS00905 0.86 -1.7 191,365 + HSERO_RS00905 0.86 -1.5 191,410 - HSERO_RS00905 0.87 +0.3 191,618 - -0.2 191,844 - -0.5 191,862 + +0.1 192,153 + HSERO_RS00910 0.11 +0.7 192,293 + HSERO_RS00910 0.37 +1.0 192,381 + HSERO_RS00910 0.53 +1.4 192,404 - HSERO_RS00910 0.58 +1.2 192,537 + HSERO_RS00910 0.82 +1.4 192,588 + +0.3 192,624 + -0.3 192,882 - HSERO_RS00915 0.14 +1.1
Or see this region's nucleotide sequence