Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21980 and HSERO_RS21985 overlap by 4 nucleotides HSERO_RS21985 and HSERO_RS21990 are separated by 22 nucleotides HSERO_RS21990 and HSERO_RS21995 are separated by 10 nucleotides
HSERO_RS21980: HSERO_RS21980 - membrane protein, at 5,028,754 to 5,030,088
_RS21980
HSERO_RS21985: HSERO_RS21985 - transcriptional regulator, at 5,030,085 to 5,030,741
_RS21985
HSERO_RS21990: HSERO_RS21990 - hypothetical protein, at 5,030,764 to 5,030,958
_RS21990
HSERO_RS21995: HSERO_RS21995 - hypothetical protein, at 5,030,969 to 5,032,129
_RS21995
Position (kb)
5030
5031 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5029.133 kb on + strand, within HSERO_RS21980 at 5029.253 kb on + strand, within HSERO_RS21980 at 5029.267 kb on - strand, within HSERO_RS21980 at 5029.298 kb on - strand, within HSERO_RS21980 at 5029.347 kb on - strand, within HSERO_RS21980 at 5029.468 kb on + strand, within HSERO_RS21980 at 5029.667 kb on - strand, within HSERO_RS21980 at 5029.703 kb on + strand, within HSERO_RS21980 at 5029.711 kb on - strand, within HSERO_RS21980 at 5029.998 kb on + strand at 5030.057 kb on - strand at 5030.093 kb on + strand at 5030.238 kb on - strand, within HSERO_RS21985 at 5030.511 kb on + strand, within HSERO_RS21985 at 5030.582 kb on + strand, within HSERO_RS21985 at 5030.613 kb on - strand, within HSERO_RS21985 at 5030.658 kb on + strand, within HSERO_RS21985 at 5030.658 kb on + strand, within HSERO_RS21985 at 5030.666 kb on - strand, within HSERO_RS21985 at 5030.686 kb on + strand at 5030.854 kb on + strand, within HSERO_RS21990 at 5030.967 kb on + strand at 5030.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 5,029,133 + HSERO_RS21980 0.28 -0.4 5,029,253 + HSERO_RS21980 0.37 +0.5 5,029,267 - HSERO_RS21980 0.38 +0.1 5,029,298 - HSERO_RS21980 0.41 +0.7 5,029,347 - HSERO_RS21980 0.44 +0.1 5,029,468 + HSERO_RS21980 0.53 +0.4 5,029,667 - HSERO_RS21980 0.68 +0.3 5,029,703 + HSERO_RS21980 0.71 -0.7 5,029,711 - HSERO_RS21980 0.72 -0.1 5,029,998 + +1.1 5,030,057 - -2.6 5,030,093 + +1.8 5,030,238 - HSERO_RS21985 0.23 +0.1 5,030,511 + HSERO_RS21985 0.65 -0.6 5,030,582 + HSERO_RS21985 0.76 -2.2 5,030,613 - HSERO_RS21985 0.80 +1.5 5,030,658 + HSERO_RS21985 0.87 -0.7 5,030,658 + HSERO_RS21985 0.87 -0.6 5,030,666 - HSERO_RS21985 0.88 -0.2 5,030,686 + +0.5 5,030,854 + HSERO_RS21990 0.46 -0.0 5,030,967 + +0.3 5,030,975 - -0.1
Or see this region's nucleotide sequence