Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS12110 and HSERO_RS12115 are separated by 116 nucleotides HSERO_RS12115 and HSERO_RS12120 overlap by 4 nucleotides HSERO_RS12120 and HSERO_RS12125 are separated by 10 nucleotides
HSERO_RS12110: HSERO_RS12110 - sugar ABC transporter substrate-binding protein, at 2,750,625 to 2,751,620
_RS12110
HSERO_RS12115: HSERO_RS12115 - ATPase, at 2,751,737 to 2,752,876
_RS12115
HSERO_RS12120: HSERO_RS12120 - ABC transporter ATP-binding protein, at 2,752,873 to 2,753,673
_RS12120
HSERO_RS12125: HSERO_RS12125 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 2,753,684 to 2,755,657
_RS12125
Position (kb)
2751
2752
2753 Strain fitness (log2 ratio)
-1
0
1 at 2751.055 kb on - strand, within HSERO_RS12110 at 2751.112 kb on - strand, within HSERO_RS12110 at 2751.196 kb on + strand, within HSERO_RS12110 at 2751.408 kb on - strand, within HSERO_RS12110 at 2751.418 kb on + strand, within HSERO_RS12110 at 2751.606 kb on + strand at 2751.663 kb on - strand at 2751.967 kb on - strand, within HSERO_RS12115 at 2752.266 kb on + strand, within HSERO_RS12115 at 2752.314 kb on - strand, within HSERO_RS12115 at 2752.334 kb on - strand, within HSERO_RS12115 at 2752.604 kb on - strand, within HSERO_RS12115 at 2752.770 kb on + strand at 2752.894 kb on + strand at 2752.923 kb on - strand at 2752.928 kb on - strand at 2753.022 kb on + strand, within HSERO_RS12120 at 2753.040 kb on + strand, within HSERO_RS12120 at 2753.076 kb on - strand, within HSERO_RS12120 at 2753.117 kb on - strand, within HSERO_RS12120 at 2753.291 kb on + strand, within HSERO_RS12120 at 2753.506 kb on - strand, within HSERO_RS12120 at 2753.534 kb on + strand, within HSERO_RS12120 at 2753.534 kb on + strand, within HSERO_RS12120 at 2753.542 kb on - strand, within HSERO_RS12120 at 2753.816 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 2,751,055 - HSERO_RS12110 0.43 +0.2 2,751,112 - HSERO_RS12110 0.49 +1.6 2,751,196 + HSERO_RS12110 0.57 -0.2 2,751,408 - HSERO_RS12110 0.79 +0.2 2,751,418 + HSERO_RS12110 0.80 +0.4 2,751,606 + +0.7 2,751,663 - +0.1 2,751,967 - HSERO_RS12115 0.20 +0.6 2,752,266 + HSERO_RS12115 0.46 +0.6 2,752,314 - HSERO_RS12115 0.51 -0.5 2,752,334 - HSERO_RS12115 0.52 +0.2 2,752,604 - HSERO_RS12115 0.76 +0.6 2,752,770 + -0.3 2,752,894 + +1.2 2,752,923 - -0.3 2,752,928 - -0.3 2,753,022 + HSERO_RS12120 0.19 -0.0 2,753,040 + HSERO_RS12120 0.21 +0.1 2,753,076 - HSERO_RS12120 0.25 -0.1 2,753,117 - HSERO_RS12120 0.30 -0.4 2,753,291 + HSERO_RS12120 0.52 +1.0 2,753,506 - HSERO_RS12120 0.79 -0.8 2,753,534 + HSERO_RS12120 0.83 +0.4 2,753,534 + HSERO_RS12120 0.83 +0.5 2,753,542 - HSERO_RS12120 0.84 -0.4 2,753,816 + -0.2
Or see this region's nucleotide sequence