Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS05325 and HSERO_RS05330 are separated by 52 nucleotides HSERO_RS05330 and HSERO_RS05335 are separated by 41 nucleotides HSERO_RS05335 and HSERO_RS05340 are separated by 14 nucleotides HSERO_RS05340 and HSERO_RS05345 are separated by 129 nucleotides
HSERO_RS05325: HSERO_RS05325 - ribose ABC transporter permease, at 1,177,238 to 1,178,224
_RS05325
HSERO_RS05330: HSERO_RS05330 - glucose dehydrogenase, at 1,178,277 to 1,179,008
_RS05330
HSERO_RS05335: HSERO_RS05335 - ribulokinase, at 1,179,050 to 1,180,696
_RS05335
HSERO_RS05340: HSERO_RS05340 - short-chain dehydrogenase, at 1,180,711 to 1,181,436
_RS05340
HSERO_RS05345: HSERO_RS05345 - lipoprotein, at 1,181,566 to 1,181,781
_RS05345
Position (kb)
1179
1180
1181 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1178.329 kb on + strand at 1178.451 kb on - strand, within HSERO_RS05330 at 1178.724 kb on - strand, within HSERO_RS05330 at 1178.756 kb on + strand, within HSERO_RS05330 at 1178.992 kb on - strand at 1179.092 kb on + strand at 1179.505 kb on + strand, within HSERO_RS05335 at 1179.514 kb on - strand, within HSERO_RS05335 at 1179.590 kb on + strand, within HSERO_RS05335 at 1179.645 kb on + strand, within HSERO_RS05335 at 1179.699 kb on + strand, within HSERO_RS05335 at 1179.704 kb on + strand, within HSERO_RS05335 at 1179.707 kb on - strand, within HSERO_RS05335 at 1179.750 kb on - strand, within HSERO_RS05335 at 1179.750 kb on - strand, within HSERO_RS05335 at 1179.800 kb on - strand, within HSERO_RS05335 at 1179.883 kb on - strand, within HSERO_RS05335 at 1180.060 kb on - strand, within HSERO_RS05335 at 1180.060 kb on - strand, within HSERO_RS05335 at 1180.065 kb on - strand, within HSERO_RS05335 at 1180.101 kb on + strand, within HSERO_RS05335 at 1180.137 kb on - strand, within HSERO_RS05335 at 1180.471 kb on + strand, within HSERO_RS05335 at 1180.509 kb on + strand, within HSERO_RS05335 at 1180.519 kb on + strand, within HSERO_RS05335 at 1180.532 kb on - strand at 1180.804 kb on - strand, within HSERO_RS05340 at 1181.079 kb on - strand, within HSERO_RS05340 at 1181.233 kb on - strand, within HSERO_RS05340 at 1181.259 kb on + strand, within HSERO_RS05340 at 1181.262 kb on - strand, within HSERO_RS05340 at 1181.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,178,329 + +0.2 1,178,451 - HSERO_RS05330 0.24 +1.3 1,178,724 - HSERO_RS05330 0.61 -0.7 1,178,756 + HSERO_RS05330 0.65 +0.4 1,178,992 - -1.2 1,179,092 + +0.8 1,179,505 + HSERO_RS05335 0.28 -0.4 1,179,514 - HSERO_RS05335 0.28 +0.3 1,179,590 + HSERO_RS05335 0.33 -3.0 1,179,645 + HSERO_RS05335 0.36 +0.1 1,179,699 + HSERO_RS05335 0.39 -1.7 1,179,704 + HSERO_RS05335 0.40 -0.3 1,179,707 - HSERO_RS05335 0.40 -0.0 1,179,750 - HSERO_RS05335 0.43 -0.3 1,179,750 - HSERO_RS05335 0.43 +1.0 1,179,800 - HSERO_RS05335 0.46 +0.4 1,179,883 - HSERO_RS05335 0.51 +1.3 1,180,060 - HSERO_RS05335 0.61 -0.3 1,180,060 - HSERO_RS05335 0.61 -0.1 1,180,065 - HSERO_RS05335 0.62 +0.3 1,180,101 + HSERO_RS05335 0.64 -0.9 1,180,137 - HSERO_RS05335 0.66 -0.4 1,180,471 + HSERO_RS05335 0.86 +0.8 1,180,509 + HSERO_RS05335 0.89 +0.1 1,180,519 + HSERO_RS05335 0.89 +0.7 1,180,532 - +0.1 1,180,804 - HSERO_RS05340 0.13 +0.1 1,181,079 - HSERO_RS05340 0.51 -0.3 1,181,233 - HSERO_RS05340 0.72 +0.9 1,181,259 + HSERO_RS05340 0.75 -0.2 1,181,262 - HSERO_RS05340 0.76 -0.5 1,181,375 - +0.3
Or see this region's nucleotide sequence