Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04340 and HSERO_RS04345 are separated by 196 nucleotides HSERO_RS04345 and HSERO_RS04350 are separated by 191 nucleotides HSERO_RS04350 and HSERO_RS04355 are separated by 298 nucleotides
HSERO_RS04340: HSERO_RS04340 - gamma-glutamyl ligase, at 958,999 to 960,228
_RS04340
HSERO_RS04345: HSERO_RS04345 - membrane protein, at 960,425 to 961,633
_RS04345
HSERO_RS04350: HSERO_RS04350 - 30S ribosomal protein S21, at 961,825 to 962,037
_RS04350
HSERO_RS04355: HSERO_RS04355 - aspartyl-tRNA amidotransferase subunit B, at 962,336 to 962,782
_RS04355
Position (kb) 960
961
962 Strain fitness (log2 ratio) -1
0
1 at 959.462 kb on + strand, within HSERO_RS04340 at 959.548 kb on + strand, within HSERO_RS04340 at 959.604 kb on - strand, within HSERO_RS04340 at 959.704 kb on + strand, within HSERO_RS04340 at 959.921 kb on - strand, within HSERO_RS04340 at 959.942 kb on + strand, within HSERO_RS04340 at 960.200 kb on + strand at 960.287 kb on + strand at 960.295 kb on - strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.390 kb on + strand at 960.535 kb on - strand at 960.553 kb on + strand, within HSERO_RS04345 at 960.694 kb on + strand, within HSERO_RS04345 at 960.786 kb on - strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 960.830 kb on + strand, within HSERO_RS04345 at 961.060 kb on + strand, within HSERO_RS04345 at 961.093 kb on - strand, within HSERO_RS04345 at 961.209 kb on + strand, within HSERO_RS04345 at 961.437 kb on + strand, within HSERO_RS04345 at 961.594 kb on + strand at 961.628 kb on - strand at 962.316 kb on + strand at 962.401 kb on + strand, within HSERO_RS04355 at 962.533 kb on + strand, within HSERO_RS04355
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 959,462 + HSERO_RS04340 0.38 -0.6 959,548 + HSERO_RS04340 0.45 -0.1 959,604 - HSERO_RS04340 0.49 -0.5 959,704 + HSERO_RS04340 0.57 +1.6 959,921 - HSERO_RS04340 0.75 +0.2 959,942 + HSERO_RS04340 0.77 -0.1 960,200 + +0.1 960,287 + -0.7 960,295 - -0.0 960,390 + -0.7 960,390 + -0.5 960,390 + +0.6 960,535 - -1.3 960,553 + HSERO_RS04345 0.11 +0.4 960,694 + HSERO_RS04345 0.22 +0.8 960,786 - HSERO_RS04345 0.30 -0.2 960,830 + HSERO_RS04345 0.33 -0.2 960,830 + HSERO_RS04345 0.33 +0.4 961,060 + HSERO_RS04345 0.53 +0.4 961,093 - HSERO_RS04345 0.55 +0.3 961,209 + HSERO_RS04345 0.65 +0.7 961,437 + HSERO_RS04345 0.84 -0.1 961,594 + -0.1 961,628 - -0.4 962,316 + -1.2 962,401 + HSERO_RS04355 0.15 +1.3 962,533 + HSERO_RS04355 0.44 -1.0
Or see this region's nucleotide sequence