Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03540 and HSERO_RS03545 are separated by 197 nucleotides HSERO_RS03545 and HSERO_RS03550 are separated by 201 nucleotides HSERO_RS03550 and HSERO_RS03555 are separated by 104 nucleotides
HSERO_RS03540: HSERO_RS03540 - dimethyladenosine transferase, at 771,352 to 772,134
_RS03540
HSERO_RS03545: HSERO_RS03545 - hypothetical protein, at 772,332 to 772,709
_RS03545
HSERO_RS03550: HSERO_RS03550 - glyoxalase I, at 772,911 to 773,315
_RS03550
HSERO_RS03555: HSERO_RS03555 - chloride channel protein, at 773,420 to 774,703
_RS03555
Position (kb)
772
773 Strain fitness (log2 ratio)
-1
0
1 at 771.575 kb on - strand, within HSERO_RS03540 at 771.951 kb on + strand, within HSERO_RS03540 at 772.250 kb on + strand at 772.304 kb on - strand at 772.397 kb on - strand, within HSERO_RS03545 at 772.413 kb on + strand, within HSERO_RS03545 at 772.557 kb on + strand, within HSERO_RS03545 at 772.656 kb on - strand, within HSERO_RS03545 at 772.746 kb on - strand at 772.773 kb on - strand at 772.793 kb on + strand at 772.794 kb on + strand at 772.802 kb on - strand at 772.980 kb on - strand, within HSERO_RS03550 at 772.992 kb on - strand, within HSERO_RS03550 at 773.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 771,575 - HSERO_RS03540 0.28 +0.8 771,951 + HSERO_RS03540 0.77 -0.3 772,250 + -0.2 772,304 - -0.1 772,397 - HSERO_RS03545 0.17 -0.7 772,413 + HSERO_RS03545 0.21 -0.7 772,557 + HSERO_RS03545 0.60 -0.9 772,656 - HSERO_RS03545 0.86 -0.1 772,746 - -0.1 772,773 - +0.1 772,793 + +1.6 772,794 + +0.9 772,802 - +0.3 772,980 - HSERO_RS03550 0.17 -0.1 772,992 - HSERO_RS03550 0.20 -0.3 773,318 - +0.5
Or see this region's nucleotide sequence