Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0029

Experiment: Varel_Bryant_medium_Glucose with Ramoplanin 0.003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0027 and BT0028 are separated by 471 nucleotidesBT0028 and BT0029 are separated by 75 nucleotidesBT0029 and BT0030 are separated by 12 nucleotides BT0027: BT0027 - putative transposase (NCBI ptt file), at 21,544 to 22,080 BT0027 BT0028: BT0028 - alkaline phosphatase (NCBI ptt file), at 22,552 to 22,830 BT0028 BT0029: BT0029 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 22,906 to 26,169 BT0029 BT0030: BT0030 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 26,182 to 28,011 BT0030 Position (kb) 22 23 24 25 26 27Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 21.957 kb on + strand, within BT0027at 21.969 kb on + strand, within BT0027at 21.969 kb on + strand, within BT0027at 22.102 kb on + strandat 22.115 kb on + strandat 22.119 kb on - strandat 22.258 kb on - strandat 22.259 kb on + strandat 22.336 kb on - strandat 22.440 kb on - strandat 22.614 kb on + strand, within BT0028at 22.634 kb on + strand, within BT0028at 22.635 kb on - strand, within BT0028at 22.635 kb on - strand, within BT0028at 22.639 kb on - strand, within BT0028at 22.650 kb on + strand, within BT0028at 22.685 kb on - strand, within BT0028at 22.690 kb on - strand, within BT0028at 22.841 kb on - strandat 22.861 kb on - strandat 22.900 kb on + strandat 23.039 kb on + strandat 23.060 kb on + strandat 23.078 kb on + strandat 23.079 kb on - strandat 23.079 kb on - strandat 23.136 kb on + strandat 23.136 kb on + strandat 23.139 kb on - strandat 23.139 kb on - strandat 23.139 kb on - strandat 23.231 kb on + strandat 23.296 kb on - strand, within BT0029at 23.329 kb on - strand, within BT0029at 23.347 kb on - strand, within BT0029at 23.412 kb on - strand, within BT0029at 23.412 kb on - strand, within BT0029at 23.543 kb on + strand, within BT0029at 23.544 kb on - strand, within BT0029at 23.586 kb on + strand, within BT0029at 23.587 kb on - strand, within BT0029at 23.631 kb on + strand, within BT0029at 23.679 kb on + strand, within BT0029at 23.687 kb on - strand, within BT0029at 23.687 kb on - strand, within BT0029at 23.739 kb on + strand, within BT0029at 23.789 kb on + strand, within BT0029at 23.789 kb on + strand, within BT0029at 23.838 kb on - strand, within BT0029at 23.850 kb on - strand, within BT0029at 23.854 kb on - strand, within BT0029at 23.888 kb on - strand, within BT0029at 23.937 kb on + strand, within BT0029at 23.940 kb on + strand, within BT0029at 23.941 kb on - strand, within BT0029at 23.969 kb on + strand, within BT0029at 23.969 kb on + strand, within BT0029at 23.970 kb on - strand, within BT0029at 24.034 kb on + strand, within BT0029at 24.034 kb on + strand, within BT0029at 24.036 kb on + strand, within BT0029at 24.036 kb on + strand, within BT0029at 24.099 kb on + strand, within BT0029at 24.112 kb on + strand, within BT0029at 24.117 kb on + strand, within BT0029at 24.124 kb on + strand, within BT0029at 24.149 kb on + strand, within BT0029at 24.193 kb on + strand, within BT0029at 24.227 kb on - strand, within BT0029at 24.271 kb on - strand, within BT0029at 24.271 kb on - strand, within BT0029at 24.273 kb on + strand, within BT0029at 24.273 kb on + strand, within BT0029at 24.298 kb on - strand, within BT0029at 24.312 kb on + strand, within BT0029at 24.328 kb on - strand, within BT0029at 24.495 kb on + strand, within BT0029at 24.510 kb on + strand, within BT0029at 24.522 kb on + strand, within BT0029at 24.568 kb on - strand, within BT0029at 24.636 kb on - strand, within BT0029at 24.640 kb on + strand, within BT0029at 24.718 kb on + strand, within BT0029at 24.757 kb on + strand, within BT0029at 24.793 kb on + strand, within BT0029at 24.794 kb on - strand, within BT0029at 24.802 kb on - strand, within BT0029at 24.906 kb on + strand, within BT0029at 24.907 kb on - strand, within BT0029at 24.907 kb on - strand, within BT0029at 24.957 kb on - strand, within BT0029at 24.988 kb on - strand, within BT0029at 24.988 kb on - strand, within BT0029at 25.027 kb on - strand, within BT0029at 25.047 kb on + strand, within BT0029at 25.074 kb on + strand, within BT0029at 25.075 kb on - strand, within BT0029at 25.157 kb on - strand, within BT0029at 25.177 kb on - strand, within BT0029at 25.192 kb on - strand, within BT0029at 25.208 kb on + strand, within BT0029at 25.208 kb on + strand, within BT0029at 25.210 kb on + strand, within BT0029at 25.211 kb on - strand, within BT0029at 25.435 kb on + strand, within BT0029at 25.480 kb on + strand, within BT0029at 25.551 kb on + strand, within BT0029at 25.578 kb on + strand, within BT0029at 25.578 kb on + strand, within BT0029at 25.579 kb on - strand, within BT0029at 25.684 kb on - strand, within BT0029at 25.699 kb on + strand, within BT0029at 25.699 kb on + strand, within BT0029at 25.700 kb on - strand, within BT0029at 25.748 kb on - strand, within BT0029at 25.750 kb on - strand, within BT0029at 25.794 kb on + strand, within BT0029at 25.795 kb on - strand, within BT0029at 25.795 kb on - strand, within BT0029at 25.795 kb on - strand, within BT0029at 25.795 kb on - strand, within BT0029at 25.803 kb on + strand, within BT0029at 25.899 kb on - strandat 25.910 kb on - strandat 25.910 kb on - strandat 25.921 kb on - strandat 26.011 kb on + strandat 26.067 kb on + strandat 26.067 kb on + strandat 26.159 kb on + strandat 26.213 kb on + strandat 26.332 kb on + strandat 26.333 kb on - strandat 26.333 kb on - strandat 26.334 kb on + strandat 26.334 kb on + strandat 26.335 kb on - strandat 26.337 kb on + strandat 26.338 kb on - strandat 26.360 kb on - strandat 26.380 kb on + strand, within BT0030at 26.423 kb on - strand, within BT0030at 26.427 kb on + strand, within BT0030at 26.569 kb on + strandat 26.570 kb on - strand, within BT0030at 26.669 kb on + strand, within BT0030at 26.793 kb on - strand, within BT0030at 26.793 kb on - strandat 26.809 kb on - strand, within BT0030at 26.812 kb on + strand, within BT0030at 26.860 kb on + strand, within BT0030at 26.860 kb on + strand, within BT0030at 26.861 kb on - strand, within BT0030at 26.948 kb on - strand, within BT0030at 26.989 kb on + strand, within BT0030at 26.991 kb on + strand, within BT0030at 26.991 kb on + strand, within BT0030at 26.992 kb on - strand, within BT0030at 27.085 kb on - strand, within BT0030at 27.102 kb on + strand, within BT0030at 27.103 kb on - strand, within BT0030at 27.103 kb on - strand, within BT0030at 27.107 kb on - strand, within BT0030at 27.107 kb on - strand, within BT0030at 27.107 kb on - strand, within BT0030at 27.111 kb on + strand, within BT0030at 27.111 kb on + strand, within BT0030at 27.111 kb on + strand, within BT0030at 27.112 kb on - strand, within BT0030

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ramoplanin 0.003125 mM
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21,957 + BT0027 0.77 -1.3
21,969 + BT0027 0.79 +1.0
21,969 + BT0027 0.79 -0.0
22,102 + -0.3
22,115 + -1.8
22,119 - -0.6
22,258 - -0.0
22,259 + -0.0
22,336 - +1.6
22,440 - -2.0
22,614 + BT0028 0.22 -0.0
22,634 + BT0028 0.29 +2.0
22,635 - BT0028 0.30 -1.0
22,635 - BT0028 0.30 -1.0
22,639 - BT0028 0.31 +0.0
22,650 + BT0028 0.35 -1.6
22,685 - BT0028 0.48 -3.5
22,690 - BT0028 0.49 +1.3
22,841 - +1.0
22,861 - -1.0
22,900 + -1.4
23,039 + -1.0
23,060 + -1.9
23,078 + -0.0
23,079 - -0.0
23,079 - -1.0
23,136 + -1.6
23,136 + -2.5
23,139 - -1.0
23,139 - -1.9
23,139 - +1.3
23,231 + -2.3
23,296 - BT0029 0.12 +0.6
23,329 - BT0029 0.13 -1.0
23,347 - BT0029 0.14 -1.8
23,412 - BT0029 0.16 -0.7
23,412 - BT0029 0.16 +1.0
23,543 + BT0029 0.20 +2.0
23,544 - BT0029 0.20 +1.0
23,586 + BT0029 0.21 -1.2
23,587 - BT0029 0.21 -0.8
23,631 + BT0029 0.22 +1.0
23,679 + BT0029 0.24 -0.8
23,687 - BT0029 0.24 -1.6
23,687 - BT0029 0.24 +2.6
23,739 + BT0029 0.26 -2.8
23,789 + BT0029 0.27 -0.0
23,789 + BT0029 0.27 +0.8
23,838 - BT0029 0.29 -0.0
23,850 - BT0029 0.29 +0.2
23,854 - BT0029 0.29 -0.0
23,888 - BT0029 0.30 +0.4
23,937 + BT0029 0.32 +0.1
23,940 + BT0029 0.32 +0.3
23,941 - BT0029 0.32 -0.4
23,969 + BT0029 0.33 -1.6
23,969 + BT0029 0.33 -0.0
23,970 - BT0029 0.33 -0.7
24,034 + BT0029 0.35 -1.0
24,034 + BT0029 0.35 -0.7
24,036 + BT0029 0.35 -1.0
24,036 + BT0029 0.35 -0.6
24,099 + BT0029 0.37 -0.6
24,112 + BT0029 0.37 -0.0
24,117 + BT0029 0.37 +1.6
24,124 + BT0029 0.37 -0.3
24,149 + BT0029 0.38 -0.8
24,193 + BT0029 0.39 -1.0
24,227 - BT0029 0.40 -2.4
24,271 - BT0029 0.42 -0.4
24,271 - BT0029 0.42 -0.0
24,273 + BT0029 0.42 -0.0
24,273 + BT0029 0.42 -0.0
24,298 - BT0029 0.43 -1.3
24,312 + BT0029 0.43 -1.9
24,328 - BT0029 0.44 -0.5
24,495 + BT0029 0.49 +0.6
24,510 + BT0029 0.49 +1.0
24,522 + BT0029 0.50 -0.4
24,568 - BT0029 0.51 +1.3
24,636 - BT0029 0.53 -1.0
24,640 + BT0029 0.53 -1.6
24,718 + BT0029 0.56 -1.6
24,757 + BT0029 0.57 +0.1
24,793 + BT0029 0.58 -1.0
24,794 - BT0029 0.58 +1.0
24,802 - BT0029 0.58 +3.1
24,906 + BT0029 0.61 +1.0
24,907 - BT0029 0.61 -0.4
24,907 - BT0029 0.61 -0.0
24,957 - BT0029 0.63 -0.8
24,988 - BT0029 0.64 -1.6
24,988 - BT0029 0.64 -0.7
25,027 - BT0029 0.65 -0.0
25,047 + BT0029 0.66 -1.3
25,074 + BT0029 0.66 +0.4
25,075 - BT0029 0.66 -2.0
25,157 - BT0029 0.69 -1.3
25,177 - BT0029 0.70 +0.6
25,192 - BT0029 0.70 -1.0
25,208 + BT0029 0.71 +1.6
25,208 + BT0029 0.71 -2.6
25,210 + BT0029 0.71 -1.0
25,211 - BT0029 0.71 -1.6
25,435 + BT0029 0.77 -1.0
25,480 + BT0029 0.79 -1.3
25,551 + BT0029 0.81 -0.0
25,578 + BT0029 0.82 +0.6
25,578 + BT0029 0.82 -1.0
25,579 - BT0029 0.82 +1.6
25,684 - BT0029 0.85 +3.4
25,699 + BT0029 0.86 -0.4
25,699 + BT0029 0.86 -2.0
25,700 - BT0029 0.86 +1.0
25,748 - BT0029 0.87 -1.6
25,750 - BT0029 0.87 -0.0
25,794 + BT0029 0.88 -1.0
25,795 - BT0029 0.89 -0.0
25,795 - BT0029 0.89 +2.3
25,795 - BT0029 0.89 -0.7
25,795 - BT0029 0.89 +0.5
25,803 + BT0029 0.89 -1.0
25,899 - -1.0
25,910 - -0.4
25,910 - -0.4
25,921 - -2.0
26,011 + -0.0
26,067 + -1.5
26,067 + -1.7
26,159 + +1.6
26,213 + -0.5
26,332 + -0.9
26,333 - +0.5
26,333 - -0.4
26,334 + -1.0
26,334 + +0.3
26,335 - -1.6
26,337 + -0.0
26,338 - -0.0
26,360 - -1.0
26,380 + BT0030 0.11 +0.8
26,423 - BT0030 0.13 +0.4
26,427 + BT0030 0.13 -2.2
26,569 + -0.0
26,570 - BT0030 0.21 +0.6
26,669 + BT0030 0.27 -1.8
26,793 - BT0030 0.33 -1.0
26,793 - -1.0
26,809 - BT0030 0.34 -0.0
26,812 + BT0030 0.34 -2.6
26,860 + BT0030 0.37 +1.0
26,860 + BT0030 0.37 -3.0
26,861 - BT0030 0.37 -0.0
26,948 - BT0030 0.42 -1.0
26,989 + BT0030 0.44 +0.6
26,991 + BT0030 0.44 -1.6
26,991 + BT0030 0.44 -1.6
26,992 - BT0030 0.44 -0.0
27,085 - BT0030 0.49 -0.0
27,102 + BT0030 0.50 -0.6
27,103 - BT0030 0.50 -0.8
27,103 - BT0030 0.50 -0.2
27,107 - BT0030 0.51 -0.0
27,107 - BT0030 0.51 -2.5
27,107 - BT0030 0.51 -0.0
27,111 + BT0030 0.51 -0.0
27,111 + BT0030 0.51 -0.9
27,111 + BT0030 0.51 -1.0
27,112 - BT0030 0.51 -0.2

Or see this region's nucleotide sequence