Experiment: Malonic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30295 and BPHYT_RS30300 are separated by 47 nucleotides BPHYT_RS30300 and BPHYT_RS30305 are separated by 2 nucleotides BPHYT_RS30305 and BPHYT_RS30310 are separated by 29 nucleotides BPHYT_RS30310 and BPHYT_RS30315 overlap by 4 nucleotides
BPHYT_RS30295: BPHYT_RS30295 - acyl-CoA dehydrogenase, at 2,353,149 to 2,354,366
_RS30295
BPHYT_RS30300: BPHYT_RS30300 - FMN reductase, at 2,354,414 to 2,354,950
_RS30300
BPHYT_RS30305: BPHYT_RS30305 - ethyl tert-butyl ether degradation EthD, at 2,354,953 to 2,355,663
_RS30305
BPHYT_RS30310: BPHYT_RS30310 - LysR family transcriptional regulator, at 2,355,693 to 2,356,643
_RS30310
BPHYT_RS30315: BPHYT_RS30315 - LysR family transcriptional regulator, at 2,356,640 to 2,357,539
_RS30315
Position (kb)
2354
2355
2356 Strain fitness (log2 ratio)
-1
0
1 at 2354.205 kb on + strand, within BPHYT_RS30295 at 2354.589 kb on - strand, within BPHYT_RS30300 at 2354.622 kb on + strand, within BPHYT_RS30300 at 2354.874 kb on - strand, within BPHYT_RS30300 at 2355.314 kb on - strand, within BPHYT_RS30305 at 2355.602 kb on - strand at 2355.633 kb on + strand at 2355.634 kb on - strand at 2355.645 kb on + strand at 2356.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Malonic (C) remove 2,354,205 + BPHYT_RS30295 0.87 +0.7 2,354,589 - BPHYT_RS30300 0.33 -0.5 2,354,622 + BPHYT_RS30300 0.39 +0.9 2,354,874 - BPHYT_RS30300 0.86 +0.9 2,355,314 - BPHYT_RS30305 0.51 +0.7 2,355,602 - +0.5 2,355,633 + +0.4 2,355,634 - +0.2 2,355,645 + +1.0 2,356,585 - +1.6
Or see this region's nucleotide sequence