Experiment: Control growth in Pro99_seawater
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3453 and HP15_3454 are separated by 39 nucleotides HP15_3454 and HP15_3455 are separated by 161 nucleotides HP15_3455 and HP15_3456 are separated by 100 nucleotides HP15_3456 and HP15_3457 are separated by 115 nucleotides
HP15_3453: HP15_3453 - cysteine synthase A, at 3,634,346 to 3,635,317
_3453
HP15_3454: HP15_3454 - OmpA domain protein transmembrane region-containing protein, at 3,635,357 to 3,635,935
_3454
HP15_3455: HP15_3455 - glycerophosphoryl diester phosphodiesterase family protein, at 3,636,097 to 3,637,371
_3455
HP15_3456: HP15_3456 - protein containing DUF465, at 3,637,472 to 3,637,729
_3456
HP15_3457: HP15_3457 - ATP dependent DNA ligase, at 3,637,845 to 3,638,699
_3457
Position (kb)
3636
3637
3638 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3635.145 kb on - strand, within HP15_3453 at 3635.221 kb on + strand at 3635.345 kb on + strand at 3635.345 kb on + strand at 3635.353 kb on - strand at 3635.355 kb on + strand at 3635.530 kb on - strand, within HP15_3454 at 3635.632 kb on + strand, within HP15_3454 at 3635.655 kb on - strand, within HP15_3454 at 3635.655 kb on - strand, within HP15_3454 at 3635.833 kb on - strand, within HP15_3454 at 3635.855 kb on + strand, within HP15_3454 at 3635.863 kb on - strand, within HP15_3454 at 3635.863 kb on - strand, within HP15_3454 at 3635.863 kb on - strand, within HP15_3454 at 3635.868 kb on - strand, within HP15_3454 at 3635.900 kb on - strand at 3635.900 kb on - strand at 3635.910 kb on - strand at 3635.953 kb on - strand at 3636.012 kb on - strand at 3636.015 kb on - strand at 3636.051 kb on + strand at 3636.210 kb on + strand at 3636.251 kb on + strand, within HP15_3455 at 3636.297 kb on + strand, within HP15_3455 at 3636.374 kb on - strand, within HP15_3455 at 3636.376 kb on + strand, within HP15_3455 at 3636.383 kb on + strand, within HP15_3455 at 3636.448 kb on - strand, within HP15_3455 at 3636.448 kb on - strand, within HP15_3455 at 3636.448 kb on - strand, within HP15_3455 at 3636.449 kb on - strand, within HP15_3455 at 3636.469 kb on + strand, within HP15_3455 at 3636.481 kb on - strand, within HP15_3455 at 3636.482 kb on - strand, within HP15_3455 at 3636.483 kb on + strand, within HP15_3455 at 3636.527 kb on + strand, within HP15_3455 at 3636.535 kb on - strand, within HP15_3455 at 3636.557 kb on - strand, within HP15_3455 at 3636.557 kb on - strand, within HP15_3455 at 3636.571 kb on - strand, within HP15_3455 at 3636.579 kb on + strand, within HP15_3455 at 3636.638 kb on - strand, within HP15_3455 at 3636.704 kb on + strand, within HP15_3455 at 3636.704 kb on + strand, within HP15_3455 at 3636.722 kb on - strand, within HP15_3455 at 3636.755 kb on + strand, within HP15_3455 at 3636.763 kb on - strand, within HP15_3455 at 3636.774 kb on + strand, within HP15_3455 at 3636.782 kb on - strand, within HP15_3455 at 3636.813 kb on + strand, within HP15_3455 at 3636.821 kb on - strand, within HP15_3455 at 3636.944 kb on - strand, within HP15_3455 at 3637.111 kb on + strand, within HP15_3455 at 3637.119 kb on - strand, within HP15_3455 at 3637.307 kb on + strand at 3637.329 kb on + strand at 3637.334 kb on + strand at 3637.572 kb on - strand, within HP15_3456 at 3637.737 kb on + strand at 3637.739 kb on + strand at 3637.739 kb on + strand at 3637.739 kb on + strand at 3637.745 kb on - strand at 3637.747 kb on - strand at 3637.747 kb on - strand at 3637.783 kb on - strand at 3637.794 kb on - strand at 3637.804 kb on + strand at 3637.804 kb on + strand at 3637.812 kb on - strand at 3637.812 kb on - strand at 3637.990 kb on + strand, within HP15_3457 at 3638.034 kb on + strand, within HP15_3457 at 3638.034 kb on + strand, within HP15_3457 at 3638.120 kb on - strand, within HP15_3457 at 3638.142 kb on - strand, within HP15_3457 at 3638.163 kb on + strand, within HP15_3457 at 3638.199 kb on - strand, within HP15_3457 at 3638.213 kb on - strand, within HP15_3457 at 3638.237 kb on + strand, within HP15_3457 at 3638.303 kb on - strand, within HP15_3457 at 3638.360 kb on + strand, within HP15_3457
Per-strain Table
Position Strand Gene LocusTag Fraction Control growth in Pro99_seawater remove 3,635,145 - HP15_3453 0.82 -3.3 3,635,221 + -0.0 3,635,345 + +0.0 3,635,345 + -2.1 3,635,353 - +1.2 3,635,355 + -1.0 3,635,530 - HP15_3454 0.30 -1.3 3,635,632 + HP15_3454 0.47 -4.0 3,635,655 - HP15_3454 0.51 -2.1 3,635,655 - HP15_3454 0.51 -0.8 3,635,833 - HP15_3454 0.82 +0.2 3,635,855 + HP15_3454 0.86 -1.1 3,635,863 - HP15_3454 0.87 +0.5 3,635,863 - HP15_3454 0.87 -4.1 3,635,863 - HP15_3454 0.87 -1.4 3,635,868 - HP15_3454 0.88 +0.3 3,635,900 - -1.5 3,635,900 - +1.8 3,635,910 - +0.8 3,635,953 - -0.2 3,636,012 - +0.9 3,636,015 - +0.4 3,636,051 + -1.0 3,636,210 + -0.8 3,636,251 + HP15_3455 0.12 -0.0 3,636,297 + HP15_3455 0.16 +0.4 3,636,374 - HP15_3455 0.22 -2.2 3,636,376 + HP15_3455 0.22 -0.9 3,636,383 + HP15_3455 0.22 +0.8 3,636,448 - HP15_3455 0.28 +0.3 3,636,448 - HP15_3455 0.28 -0.2 3,636,448 - HP15_3455 0.28 -2.1 3,636,449 - HP15_3455 0.28 -0.6 3,636,469 + HP15_3455 0.29 +0.6 3,636,481 - HP15_3455 0.30 -0.1 3,636,482 - HP15_3455 0.30 -3.4 3,636,483 + HP15_3455 0.30 +0.3 3,636,527 + HP15_3455 0.34 -2.1 3,636,535 - HP15_3455 0.34 -3.8 3,636,557 - HP15_3455 0.36 +0.0 3,636,557 - HP15_3455 0.36 -1.6 3,636,571 - HP15_3455 0.37 -0.3 3,636,579 + HP15_3455 0.38 -3.3 3,636,638 - HP15_3455 0.42 +0.4 3,636,704 + HP15_3455 0.48 +0.1 3,636,704 + HP15_3455 0.48 +0.5 3,636,722 - HP15_3455 0.49 -2.4 3,636,755 + HP15_3455 0.52 -0.4 3,636,763 - HP15_3455 0.52 +0.7 3,636,774 + HP15_3455 0.53 +0.9 3,636,782 - HP15_3455 0.54 +0.0 3,636,813 + HP15_3455 0.56 -2.5 3,636,821 - HP15_3455 0.57 -0.0 3,636,944 - HP15_3455 0.66 +0.6 3,637,111 + HP15_3455 0.80 +0.9 3,637,119 - HP15_3455 0.80 -0.9 3,637,307 + +0.1 3,637,329 + -0.6 3,637,334 + +0.9 3,637,572 - HP15_3456 0.39 -0.4 3,637,737 + +0.4 3,637,739 + -0.2 3,637,739 + +0.2 3,637,739 + -2.8 3,637,745 - -3.9 3,637,747 - +0.3 3,637,747 - +0.0 3,637,783 - +0.2 3,637,794 - +0.0 3,637,804 + +0.0 3,637,804 + -0.8 3,637,812 - -2.4 3,637,812 - +0.5 3,637,990 + HP15_3457 0.17 -1.3 3,638,034 + HP15_3457 0.22 +0.1 3,638,034 + HP15_3457 0.22 -0.4 3,638,120 - HP15_3457 0.32 +0.7 3,638,142 - HP15_3457 0.35 -0.1 3,638,163 + HP15_3457 0.37 +0.0 3,638,199 - HP15_3457 0.41 -0.1 3,638,213 - HP15_3457 0.43 +0.2 3,638,237 + HP15_3457 0.46 -2.2 3,638,303 - HP15_3457 0.54 -0.2 3,638,360 + HP15_3457 0.60 -0.0
Or see this region's nucleotide sequence