Experiment: RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 0.25x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20140 and BWI76_RS20145 overlap by 4 nucleotides BWI76_RS20145 and BWI76_RS20150 overlap by 4 nucleotides BWI76_RS20150 and BWI76_RS20155 are separated by 10 nucleotides BWI76_RS20155 and BWI76_RS20160 are separated by 14 nucleotides BWI76_RS20160 and BWI76_RS20165 overlap by 4 nucleotides
BWI76_RS20140: BWI76_RS20140 - NADH-quinone oxidoreductase subunit L, at 4,220,138 to 4,221,979
_RS20140
BWI76_RS20145: BWI76_RS20145 - NADH-quinone oxidoreductase subunit K, at 4,221,976 to 4,222,278
_RS20145
BWI76_RS20150: BWI76_RS20150 - NADH:ubiquinone oxidoreductase subunit J, at 4,222,275 to 4,222,829
_RS20150
BWI76_RS20155: BWI76_RS20155 - NADH-quinone oxidoreductase subunit I, at 4,222,840 to 4,223,382
_RS20155
BWI76_RS20160: BWI76_RS20160 - NADH-quinone oxidoreductase subunit H, at 4,223,397 to 4,224,374
_RS20160
BWI76_RS20165: BWI76_RS20165 - NADH-quinone oxidoreductase subunit G, at 4,224,371 to 4,227,097
_RS20165
Position (kb) 4222
4223
4224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4222.044 kb on + strand, within BWI76_RS20145 at 4222.323 kb on + strand at 4222.604 kb on + strand, within BWI76_RS20150 at 4222.645 kb on - strand, within BWI76_RS20150 at 4222.756 kb on + strand, within BWI76_RS20150 at 4222.891 kb on + strand at 4222.899 kb on - strand, within BWI76_RS20155 at 4223.157 kb on + strand, within BWI76_RS20155 at 4223.190 kb on + strand, within BWI76_RS20155 at 4223.388 kb on + strand at 4223.447 kb on + strand at 4223.659 kb on + strand, within BWI76_RS20160 at 4223.804 kb on - strand, within BWI76_RS20160 at 4224.021 kb on + strand, within BWI76_RS20160 at 4224.218 kb on - strand, within BWI76_RS20160
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 0.25x remove 4,222,044 + BWI76_RS20145 0.22 +0.2 4,222,323 + +0.6 4,222,604 + BWI76_RS20150 0.59 -0.9 4,222,645 - BWI76_RS20150 0.67 +0.1 4,222,756 + BWI76_RS20150 0.87 -0.3 4,222,891 + +1.0 4,222,899 - BWI76_RS20155 0.11 +0.6 4,223,157 + BWI76_RS20155 0.58 -2.2 4,223,190 + BWI76_RS20155 0.64 +1.0 4,223,388 + -0.2 4,223,447 + +0.0 4,223,659 + BWI76_RS20160 0.27 -0.4 4,223,804 - BWI76_RS20160 0.42 +0.8 4,224,021 + BWI76_RS20160 0.64 +1.8 4,224,218 - BWI76_RS20160 0.84 -1.2
Or see this region's nucleotide sequence