Experiment: RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 0.25x 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt BWI76_RS16020 and BWI76_RS16025 overlap by 1 nucleotides BWI76_RS16025 and BWI76_RS16030 are separated by 17 nucleotides BWI76_RS16030 and BWI76_RS16035 overlap by 8 nucleotides BWI76_RS16035 and BWI76_RS16040 overlap by 1 nucleotides 
        BWI76_RS16020: BWI76_RS16020 - malonate decarboxylase subunit alpha, at 3,322,117 to 3,323,772 
        _RS16020 
         
        
        BWI76_RS16025: BWI76_RS16025 - triphosphoribosyl-dephospho-CoA synthase, at 3,323,772 to 3,324,614 
        _RS16025 
         
        
        BWI76_RS16030: BWI76_RS16030 - malonate decarboxylase acyl carrier protein, at 3,324,632 to 3,324,931 
        _RS16030 
         
        
        BWI76_RS16035: BWI76_RS16035 - biotin-independent malonate decarboxylase subunit beta, at 3,324,924 to 3,325,757 
        _RS16035 
         
        
        BWI76_RS16040: BWI76_RS16040 - biotin-independent malonate decarboxylase subunit gamma, at 3,325,757 to 3,326,557 
        _RS16040 
         Position (kb) 3324 
3325 Strain fitness (log2 ratio) -1 
0 
1 at 3323.737 kb on + strand at 3324.104 kb on - strand, within BWI76_RS16025 at 3324.304 kb on - strand, within BWI76_RS16025 at 3324.729 kb on - strand, within BWI76_RS16030 at 3324.776 kb on - strand, within BWI76_RS16030 at 3324.906 kb on + strand at 3324.991 kb on - strand at 3325.238 kb on + strand, within BWI76_RS16035 at 3325.287 kb on - strand, within BWI76_RS16035 at 3325.339 kb on + strand, within BWI76_RS16035  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 0.25x remove 3,323,737 +  -1.0 3,324,104 -  BWI76_RS16025 0.39  -0.4 3,324,304 -  BWI76_RS16025 0.63  +0.3 3,324,729 -  BWI76_RS16030 0.32  -0.9 3,324,776 -  BWI76_RS16030 0.48  +0.7 3,324,906 +  -0.6 3,324,991 -  +0.4 3,325,238 +  BWI76_RS16035 0.38  -0.9 3,325,287 -  BWI76_RS16035 0.44  +0.4 3,325,339 +  BWI76_RS16035 0.50  -0.8 
Or see this region's nucleotide sequence