Experiment: LB lactate, anaerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4205 and SO4206 overlap by 1 nucleotides SO4206 and SO4207 overlap by 8 nucleotides SO4207 and hemB-2 are separated by 9 nucleotides
SO4205: SO4205 - hypothetical protein (NCBI ptt file), at 4,371,387 to 4,371,908
SO4205
SO4206: SO4206 - hydrolase, TatD family (NCBI ptt file), at 4,371,908 to 4,372,711
SO4206
SO4207: SO4207 - GGDEF domain protein (NCBI ptt file), at 4,372,704 to 4,374,581
SO4207
SO4208: hemB-2 - delta-aminolevulinic acid dehydratase (NCBI ptt file), at 4,374,591 to 4,375,601
hemB-2
Position (kb)
4372
4373
4374
4375 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4371.706 kb on - strand, within SO4205 at 4371.706 kb on - strand, within SO4205 at 4371.802 kb on + strand, within SO4205 at 4371.884 kb on + strand at 4371.889 kb on + strand at 4371.889 kb on + strand at 4372.197 kb on + strand, within SO4206 at 4372.232 kb on + strand, within SO4206 at 4372.232 kb on + strand, within SO4206 at 4372.240 kb on - strand, within SO4206 at 4372.257 kb on + strand, within SO4206 at 4372.375 kb on - strand, within SO4206 at 4372.377 kb on + strand, within SO4206 at 4372.385 kb on - strand, within SO4206 at 4372.399 kb on + strand, within SO4206 at 4372.442 kb on + strand, within SO4206 at 4372.450 kb on - strand, within SO4206 at 4372.530 kb on + strand, within SO4206 at 4372.558 kb on + strand, within SO4206 at 4372.621 kb on - strand, within SO4206 at 4372.780 kb on + strand at 4372.795 kb on + strand at 4372.795 kb on + strand at 4372.838 kb on + strand at 4372.976 kb on - strand, within SO4207 at 4372.981 kb on + strand, within SO4207 at 4373.048 kb on + strand, within SO4207 at 4373.083 kb on + strand, within SO4207 at 4373.095 kb on + strand, within SO4207 at 4373.103 kb on - strand, within SO4207 at 4373.260 kb on + strand, within SO4207 at 4373.338 kb on + strand, within SO4207 at 4373.556 kb on + strand, within SO4207 at 4373.564 kb on - strand, within SO4207 at 4373.564 kb on - strand, within SO4207 at 4373.595 kb on - strand, within SO4207 at 4373.642 kb on - strand, within SO4207 at 4373.702 kb on + strand, within SO4207 at 4373.710 kb on - strand, within SO4207 at 4373.717 kb on - strand, within SO4207 at 4373.740 kb on - strand, within SO4207 at 4373.750 kb on - strand, within SO4207 at 4373.807 kb on - strand, within SO4207 at 4373.819 kb on - strand, within SO4207 at 4373.847 kb on - strand, within SO4207 at 4373.865 kb on - strand, within SO4207 at 4373.877 kb on + strand, within SO4207 at 4373.878 kb on - strand, within SO4207 at 4374.001 kb on + strand, within SO4207 at 4374.001 kb on + strand, within SO4207 at 4374.329 kb on + strand, within SO4207 at 4374.436 kb on + strand at 4374.456 kb on - strand at 4374.468 kb on + strand at 4374.468 kb on + strand at 4374.476 kb on - strand at 4374.478 kb on + strand at 4374.515 kb on - strand at 4374.610 kb on + strand at 4374.767 kb on + strand, within hemB-2 at 4375.001 kb on - strand, within hemB-2 at 4375.020 kb on + strand, within hemB-2 at 4375.127 kb on + strand, within hemB-2 at 4375.194 kb on + strand, within hemB-2 at 4375.202 kb on - strand, within hemB-2 at 4375.321 kb on - strand, within hemB-2 at 4375.355 kb on - strand, within hemB-2 at 4375.379 kb on + strand, within hemB-2 at 4375.416 kb on + strand, within hemB-2 at 4375.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB lactate, anaerobic, 1G configuration remove 4,371,706 - SO4205 0.61 -0.4 4,371,706 - SO4205 0.61 -0.6 4,371,802 + SO4205 0.80 -2.4 4,371,884 + -0.8 4,371,889 + -1.1 4,371,889 + -1.8 4,372,197 + SO4206 0.36 -1.5 4,372,232 + SO4206 0.40 -1.2 4,372,232 + SO4206 0.40 -0.8 4,372,240 - SO4206 0.41 -1.9 4,372,257 + SO4206 0.43 -3.8 4,372,375 - SO4206 0.58 -2.5 4,372,377 + SO4206 0.58 -0.8 4,372,385 - SO4206 0.59 +0.4 4,372,399 + SO4206 0.61 -1.1 4,372,442 + SO4206 0.66 -2.1 4,372,450 - SO4206 0.67 -1.9 4,372,530 + SO4206 0.77 -0.9 4,372,558 + SO4206 0.81 -0.6 4,372,621 - SO4206 0.89 -0.6 4,372,780 + -0.1 4,372,795 + -0.2 4,372,795 + +0.9 4,372,838 + -0.9 4,372,976 - SO4207 0.14 -1.0 4,372,981 + SO4207 0.15 -1.4 4,373,048 + SO4207 0.18 -0.5 4,373,083 + SO4207 0.20 -0.5 4,373,095 + SO4207 0.21 -1.8 4,373,103 - SO4207 0.21 -1.4 4,373,260 + SO4207 0.30 -0.9 4,373,338 + SO4207 0.34 -1.7 4,373,556 + SO4207 0.45 -1.3 4,373,564 - SO4207 0.46 -0.8 4,373,564 - SO4207 0.46 -0.6 4,373,595 - SO4207 0.47 -1.8 4,373,642 - SO4207 0.50 -0.9 4,373,702 + SO4207 0.53 +0.4 4,373,710 - SO4207 0.54 -1.1 4,373,717 - SO4207 0.54 -3.2 4,373,740 - SO4207 0.55 -2.5 4,373,750 - SO4207 0.56 -0.5 4,373,807 - SO4207 0.59 -1.3 4,373,819 - SO4207 0.59 -0.4 4,373,847 - SO4207 0.61 -1.5 4,373,865 - SO4207 0.62 -1.4 4,373,877 + SO4207 0.62 -0.7 4,373,878 - SO4207 0.63 -0.4 4,374,001 + SO4207 0.69 -0.9 4,374,001 + SO4207 0.69 -1.5 4,374,329 + SO4207 0.87 -1.0 4,374,436 + -1.7 4,374,456 - -1.5 4,374,468 + -0.1 4,374,468 + -0.9 4,374,476 - -1.1 4,374,478 + -0.4 4,374,515 - -1.3 4,374,610 + -0.3 4,374,767 + hemB-2 SO4208 0.17 -1.2 4,375,001 - hemB-2 SO4208 0.41 -0.4 4,375,020 + hemB-2 SO4208 0.42 -0.5 4,375,127 + hemB-2 SO4208 0.53 +0.0 4,375,194 + hemB-2 SO4208 0.60 -0.3 4,375,202 - hemB-2 SO4208 0.60 -0.4 4,375,321 - hemB-2 SO4208 0.72 -0.5 4,375,355 - hemB-2 SO4208 0.76 -0.1 4,375,379 + hemB-2 SO4208 0.78 -0.6 4,375,416 + hemB-2 SO4208 0.82 -0.3 4,375,515 + +1.3
Or see this region's nucleotide sequence