Experiment: LB lactate, anaerobic, 1G configuration
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3269 and SO3270 are separated by 15 nucleotides SO3270 and SO3271 overlap by 4 nucleotides
SO3269: SO3269 - cytidylyltransferase domain protein (NCBI ptt file), at 3,406,993 to 3,408,717
SO3269
SO3270: SO3270 - polysaccharide biosynthesis protein (NCBI ptt file), at 3,408,733 to 3,409,878
SO3270
SO3271: SO3271 - polysaccharide biosynthesis protein (NCBI ptt file), at 3,409,875 to 3,410,870
SO3271
Position (kb)
3408
3409
3410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3407.737 kb on - strand, within SO3269 at 3407.769 kb on + strand, within SO3269 at 3407.854 kb on + strand, within SO3269 at 3407.896 kb on + strand, within SO3269 at 3407.947 kb on + strand, within SO3269 at 3407.947 kb on + strand, within SO3269 at 3408.038 kb on + strand, within SO3269 at 3408.046 kb on - strand, within SO3269 at 3408.060 kb on - strand, within SO3269 at 3408.060 kb on - strand, within SO3269 at 3408.070 kb on - strand, within SO3269 at 3408.130 kb on + strand, within SO3269 at 3408.140 kb on + strand, within SO3269 at 3408.240 kb on + strand, within SO3269 at 3408.271 kb on - strand, within SO3269 at 3408.341 kb on - strand, within SO3269 at 3408.392 kb on + strand, within SO3269 at 3408.434 kb on + strand, within SO3269 at 3408.442 kb on - strand, within SO3269 at 3408.522 kb on - strand, within SO3269 at 3408.625 kb on + strand at 3408.633 kb on - strand at 3408.633 kb on - strand at 3409.103 kb on - strand, within SO3270 at 3409.138 kb on - strand, within SO3270 at 3409.138 kb on - strand, within SO3270 at 3409.183 kb on - strand, within SO3270 at 3409.371 kb on - strand, within SO3270 at 3409.499 kb on + strand, within SO3270 at 3409.499 kb on + strand, within SO3270 at 3409.507 kb on - strand, within SO3270 at 3409.785 kb on + strand at 3409.785 kb on + strand at 3409.785 kb on + strand at 3409.786 kb on + strand at 3409.792 kb on - strand at 3409.819 kb on - strand at 3409.877 kb on + strand at 3409.890 kb on - strand at 3410.088 kb on - strand, within SO3271 at 3410.091 kb on - strand, within SO3271 at 3410.229 kb on - strand, within SO3271 at 3410.253 kb on + strand, within SO3271 at 3410.474 kb on - strand, within SO3271 at 3410.506 kb on + strand, within SO3271 at 3410.595 kb on + strand, within SO3271 at 3410.769 kb on + strand, within SO3271 at 3410.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB lactate, anaerobic, 1G configuration remove 3,407,737 - SO3269 0.43 -0.2 3,407,769 + SO3269 0.45 +0.0 3,407,854 + SO3269 0.50 -0.8 3,407,896 + SO3269 0.52 -1.2 3,407,947 + SO3269 0.55 -2.2 3,407,947 + SO3269 0.55 -2.6 3,408,038 + SO3269 0.61 -2.4 3,408,046 - SO3269 0.61 -1.0 3,408,060 - SO3269 0.62 -0.7 3,408,060 - SO3269 0.62 -1.8 3,408,070 - SO3269 0.62 -1.9 3,408,130 + SO3269 0.66 -1.5 3,408,140 + SO3269 0.66 +0.2 3,408,240 + SO3269 0.72 -2.1 3,408,271 - SO3269 0.74 -2.0 3,408,341 - SO3269 0.78 +0.4 3,408,392 + SO3269 0.81 -2.4 3,408,434 + SO3269 0.84 -1.6 3,408,442 - SO3269 0.84 -3.1 3,408,522 - SO3269 0.89 -1.0 3,408,625 + -2.1 3,408,633 - -1.0 3,408,633 - -1.6 3,409,103 - SO3270 0.32 -1.3 3,409,138 - SO3270 0.35 -3.0 3,409,138 - SO3270 0.35 -0.9 3,409,183 - SO3270 0.39 +2.0 3,409,371 - SO3270 0.56 -1.0 3,409,499 + SO3270 0.67 -1.7 3,409,499 + SO3270 0.67 -1.9 3,409,507 - SO3270 0.68 -2.4 3,409,785 + -2.6 3,409,785 + -1.8 3,409,785 + -1.6 3,409,786 + -2.8 3,409,792 - -0.6 3,409,819 - +0.6 3,409,877 + -0.6 3,409,890 - -0.6 3,410,088 - SO3271 0.21 -0.2 3,410,091 - SO3271 0.22 -3.5 3,410,229 - SO3271 0.36 +1.0 3,410,253 + SO3271 0.38 -0.6 3,410,474 - SO3271 0.60 -2.1 3,410,506 + SO3271 0.63 -1.8 3,410,595 + SO3271 0.72 -0.4 3,410,769 + SO3271 0.90 -2.1 3,410,782 + -0.6
Or see this region's nucleotide sequence