Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS10065 and HSERO_RS10070 are separated by 93 nucleotides HSERO_RS10070 and HSERO_RS10075 are separated by 37 nucleotides HSERO_RS10075 and HSERO_RS10080 are separated by 303 nucleotides HSERO_RS10080 and HSERO_RS10085 are separated by 296 nucleotides
HSERO_RS10065: HSERO_RS10065 - hypothetical protein, at 2,277,145 to 2,277,924
_RS10065
HSERO_RS10070: HSERO_RS10070 - hypothetical protein, at 2,278,018 to 2,278,419
_RS10070
HSERO_RS10075: HSERO_RS10075 - tRNA-Pro, at 2,278,457 to 2,278,533
_RS10075
HSERO_RS10080: HSERO_RS10080 - hypothetical protein, at 2,278,837 to 2,279,043
_RS10080
HSERO_RS10085: HSERO_RS10085 - transcriptional regulator, at 2,279,340 to 2,279,969
_RS10085
Position (kb)
2278
2279 Strain fitness (log2 ratio)
-1
0
1 at 2277.730 kb on + strand, within HSERO_RS10065 at 2277.779 kb on + strand, within HSERO_RS10065 at 2278.024 kb on + strand at 2278.204 kb on - strand, within HSERO_RS10070 at 2278.311 kb on + strand, within HSERO_RS10070 at 2278.579 kb on + strand at 2278.633 kb on - strand at 2278.810 kb on + strand at 2278.924 kb on + strand at 2278.968 kb on - strand, within HSERO_RS10080 at 2278.978 kb on - strand, within HSERO_RS10080 at 2279.191 kb on - strand at 2279.226 kb on - strand at 2279.234 kb on + strand at 2279.236 kb on - strand at 2279.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 2,277,730 + HSERO_RS10065 0.75 -0.1 2,277,779 + HSERO_RS10065 0.81 -1.0 2,278,024 + +0.1 2,278,204 - HSERO_RS10070 0.46 +1.1 2,278,311 + HSERO_RS10070 0.73 +0.4 2,278,579 + -0.1 2,278,633 - -0.1 2,278,810 + +0.1 2,278,924 + +0.3 2,278,968 - HSERO_RS10080 0.63 -0.3 2,278,978 - HSERO_RS10080 0.68 -0.3 2,279,191 - -0.5 2,279,226 - +0.6 2,279,234 + -0.3 2,279,236 - -0.1 2,279,258 - -0.5
Or see this region's nucleotide sequence