Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09390 and PGA1_c09400 are separated by 156 nucleotides PGA1_c09400 and PGA1_c09410 are separated by 235 nucleotides PGA1_c09410 and PGA1_c09420 are separated by 115 nucleotides
PGA1_c09390: PGA1_c09390 - modification methylase NaeI, at 957,663 to 958,637
_c09390
PGA1_c09400: PGA1_c09400 - tRNA-Gly, at 958,794 to 958,868
_c09400
PGA1_c09410: PGA1_c09410 - hypothetical protein, at 959,104 to 959,310
_c09410
PGA1_c09420: PGA1_c09420 - pyruvate carboxylase Pyc, at 959,426 to 962,992
_c09420
Position (kb) 959
960 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 958.716 kb on + strand at 958.716 kb on + strand at 958.716 kb on + strand at 958.724 kb on - strand at 958.906 kb on - strand at 959.112 kb on + strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.120 kb on - strand at 959.168 kb on - strand, within PGA1_c09410 at 959.168 kb on - strand, within PGA1_c09410 at 959.213 kb on + strand, within PGA1_c09410 at 959.294 kb on + strand at 959.304 kb on + strand at 959.304 kb on + strand at 959.312 kb on - strand at 959.312 kb on - strand at 959.402 kb on + strand at 959.429 kb on + strand at 959.487 kb on + strand at 959.487 kb on + strand at 959.487 kb on + strand at 959.495 kb on - strand at 959.495 kb on - strand at 959.495 kb on - strand at 959.600 kb on + strand at 959.607 kb on + strand at 959.610 kb on + strand at 959.645 kb on - strand at 959.658 kb on + strand at 959.666 kb on - strand at 959.669 kb on + strand at 959.684 kb on - strand at 959.748 kb on + strand at 959.756 kb on - strand at 959.833 kb on + strand, within PGA1_c09420 at 959.833 kb on + strand, within PGA1_c09420 at 959.841 kb on - strand, within PGA1_c09420 at 959.905 kb on + strand, within PGA1_c09420 at 959.976 kb on + strand, within PGA1_c09420 at 959.994 kb on - strand, within PGA1_c09420 at 959.994 kb on - strand, within PGA1_c09420 at 960.002 kb on - strand, within PGA1_c09420 at 960.082 kb on + strand, within PGA1_c09420 at 960.169 kb on - strand, within PGA1_c09420 at 960.185 kb on + strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.193 kb on - strand, within PGA1_c09420 at 960.198 kb on - strand, within PGA1_c09420 at 960.218 kb on + strand, within PGA1_c09420 at 960.229 kb on + strand, within PGA1_c09420 at 960.282 kb on + strand, within PGA1_c09420 at 960.282 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420 at 960.287 kb on + strand, within PGA1_c09420
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 958,716 + -0.8 958,716 + +0.5 958,716 + -0.6 958,724 - -2.4 958,906 - -0.7 959,112 + +0.8 959,120 - +1.2 959,120 - -0.2 959,120 - +0.1 959,120 - +1.0 959,168 - PGA1_c09410 0.31 +0.7 959,168 - PGA1_c09410 0.31 -0.8 959,213 + PGA1_c09410 0.53 +1.0 959,294 + -1.5 959,304 + -0.5 959,304 + -0.5 959,312 - +1.0 959,312 - +0.1 959,402 + +0.1 959,429 + -2.0 959,487 + -1.5 959,487 + -1.0 959,487 + -1.8 959,495 - -2.2 959,495 - -2.5 959,495 - -0.5 959,600 + -2.8 959,607 + -1.6 959,610 + -3.7 959,645 - -2.9 959,658 + -2.3 959,666 - -2.3 959,669 + -0.5 959,684 - -3.3 959,748 + -2.5 959,756 - -2.5 959,833 + PGA1_c09420 0.11 -0.8 959,833 + PGA1_c09420 0.11 -2.0 959,841 - PGA1_c09420 0.12 -1.8 959,905 + PGA1_c09420 0.13 -1.5 959,976 + PGA1_c09420 0.15 -3.0 959,994 - PGA1_c09420 0.16 -0.5 959,994 - PGA1_c09420 0.16 -2.8 960,002 - PGA1_c09420 0.16 -0.0 960,082 + PGA1_c09420 0.18 -1.8 960,169 - PGA1_c09420 0.21 -1.9 960,185 + PGA1_c09420 0.21 -1.0 960,193 - PGA1_c09420 0.22 -1.9 960,193 - PGA1_c09420 0.22 -1.8 960,193 - PGA1_c09420 0.22 -1.8 960,198 - PGA1_c09420 0.22 -0.0 960,218 + PGA1_c09420 0.22 -2.5 960,229 + PGA1_c09420 0.23 -2.5 960,282 + PGA1_c09420 0.24 -2.8 960,282 + PGA1_c09420 0.24 -1.0 960,287 + PGA1_c09420 0.24 -1.5 960,287 + PGA1_c09420 0.24 -2.0 960,287 + PGA1_c09420 0.24 -2.8 960,287 + PGA1_c09420 0.24 -1.3 960,287 + PGA1_c09420 0.24 -1.8
Or see this region's nucleotide sequence