Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00190 and PGA1_78p00200 are separated by 20 nucleotides PGA1_78p00200 and PGA1_78p00210 are separated by 9 nucleotides PGA1_78p00210 and PGA1_78p00220 are separated by 49 nucleotides
PGA1_78p00190: PGA1_78p00190 - sn-glycerol-3-phosphate import ATP-binding protein UgbC, at 22,202 to 23,287
_78p00190
PGA1_78p00200: PGA1_78p00200 - Sugar phosphate isomerases/epimerases, at 23,308 to 24,051
_78p00200
PGA1_78p00210: PGA1_78p00210 - putative oxidoreductase, at 24,061 to 25,194
_78p00210
PGA1_78p00220: PGA1_78p00220 - transcriptional regulatory protein, LysR family, at 25,244 to 26,131
_78p00220
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1
2 at 23.154 kb on - strand, within PGA1_78p00190 at 23.781 kb on - strand, within PGA1_78p00200 at 23.786 kb on - strand, within PGA1_78p00200 at 24.221 kb on + strand, within PGA1_78p00210 at 24.532 kb on - strand, within PGA1_78p00210 at 24.597 kb on + strand, within PGA1_78p00210 at 24.853 kb on - strand, within PGA1_78p00210 at 25.032 kb on + strand, within PGA1_78p00210 at 25.362 kb on + strand, within PGA1_78p00220 at 25.560 kb on - strand, within PGA1_78p00220 at 26.110 kb on + strand at 26.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 23,154 - PGA1_78p00190 0.88 -1.5 23,781 - PGA1_78p00200 0.64 -2.7 23,786 - PGA1_78p00200 0.64 +0.5 24,221 + PGA1_78p00210 0.14 +1.5 24,532 - PGA1_78p00210 0.42 -0.2 24,597 + PGA1_78p00210 0.47 +1.5 24,853 - PGA1_78p00210 0.70 +0.8 25,032 + PGA1_78p00210 0.86 +2.1 25,362 + PGA1_78p00220 0.13 -2.3 25,560 - PGA1_78p00220 0.36 -0.0 26,110 + +0.5 26,118 - -1.8
Or see this region's nucleotide sequence