Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24430 and PGA1_c24440 are separated by 149 nucleotides PGA1_c24440 and PGA1_c24450 are separated by 110 nucleotides PGA1_c24450 and PGA1_c24460 are separated by 214 nucleotides PGA1_c24460 and PGA1_c24470 overlap by 4 nucleotides
PGA1_c24430: PGA1_c24430 - peptidase M48-like protein, at 2,547,833 to 2,548,558
_c24430
PGA1_c24440: PGA1_c24440 - hypothetical protein, at 2,548,708 to 2,548,956
_c24440
PGA1_c24450: PGA1_c24450 - Uncharacterized conserved protein, at 2,549,067 to 2,549,363
_c24450
PGA1_c24460: PGA1_c24460 - putative nitroreductase, at 2,549,578 to 2,550,156
_c24460
PGA1_c24470: PGA1_c24470 - Uncharacterized protein involved in cysteine biosynthesis, at 2,550,153 to 2,550,857
_c24470
Position (kb)
2549
2550 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2548.070 kb on + strand, within PGA1_c24430 at 2548.078 kb on - strand, within PGA1_c24430 at 2548.121 kb on + strand, within PGA1_c24430 at 2548.167 kb on + strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.266 kb on - strand, within PGA1_c24430 at 2548.288 kb on - strand, within PGA1_c24430 at 2548.290 kb on + strand, within PGA1_c24430 at 2548.291 kb on - strand, within PGA1_c24430 at 2548.298 kb on - strand, within PGA1_c24430 at 2548.340 kb on + strand, within PGA1_c24430 at 2548.348 kb on - strand, within PGA1_c24430 at 2548.372 kb on - strand, within PGA1_c24430 at 2548.413 kb on + strand, within PGA1_c24430 at 2548.429 kb on - strand, within PGA1_c24430 at 2548.436 kb on - strand, within PGA1_c24430 at 2548.439 kb on + strand, within PGA1_c24430 at 2548.447 kb on - strand, within PGA1_c24430 at 2548.447 kb on - strand, within PGA1_c24430 at 2548.503 kb on - strand at 2548.533 kb on - strand at 2548.535 kb on - strand at 2548.556 kb on + strand at 2548.612 kb on - strand at 2548.626 kb on + strand at 2548.634 kb on - strand at 2548.702 kb on + strand at 2548.707 kb on + strand at 2548.759 kb on + strand, within PGA1_c24440 at 2548.835 kb on - strand, within PGA1_c24440 at 2548.856 kb on + strand, within PGA1_c24440 at 2548.897 kb on + strand, within PGA1_c24440 at 2548.898 kb on + strand, within PGA1_c24440 at 2548.905 kb on - strand, within PGA1_c24440 at 2548.905 kb on - strand, within PGA1_c24440 at 2548.906 kb on - strand, within PGA1_c24440 at 2548.908 kb on + strand, within PGA1_c24440 at 2548.908 kb on + strand, within PGA1_c24440 at 2549.042 kb on - strand at 2549.154 kb on - strand, within PGA1_c24450 at 2549.168 kb on - strand, within PGA1_c24450 at 2549.229 kb on - strand, within PGA1_c24450 at 2549.244 kb on - strand, within PGA1_c24450 at 2549.276 kb on + strand, within PGA1_c24450 at 2549.281 kb on + strand, within PGA1_c24450 at 2549.333 kb on - strand, within PGA1_c24450 at 2549.333 kb on - strand, within PGA1_c24450 at 2549.358 kb on + strand at 2549.366 kb on - strand at 2549.476 kb on - strand at 2549.532 kb on + strand at 2549.589 kb on + strand at 2549.597 kb on - strand at 2549.611 kb on + strand at 2549.635 kb on + strand at 2549.635 kb on + strand at 2549.695 kb on + strand, within PGA1_c24460 at 2549.705 kb on + strand, within PGA1_c24460 at 2549.705 kb on + strand, within PGA1_c24460 at 2549.852 kb on - strand, within PGA1_c24460 at 2549.880 kb on - strand, within PGA1_c24460 at 2549.902 kb on - strand, within PGA1_c24460 at 2549.919 kb on + strand, within PGA1_c24460 at 2549.919 kb on + strand, within PGA1_c24460 at 2549.919 kb on + strand, within PGA1_c24460 at 2549.927 kb on - strand, within PGA1_c24460 at 2549.927 kb on - strand, within PGA1_c24460 at 2549.942 kb on + strand, within PGA1_c24460 at 2549.942 kb on + strand, within PGA1_c24460 at 2550.041 kb on + strand, within PGA1_c24460 at 2550.335 kb on - strand, within PGA1_c24470
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 2,548,070 + PGA1_c24430 0.33 -2.4 2,548,078 - PGA1_c24430 0.34 -2.2 2,548,121 + PGA1_c24430 0.40 -2.9 2,548,167 + PGA1_c24430 0.46 +0.8 2,548,175 - PGA1_c24430 0.47 -0.8 2,548,175 - PGA1_c24430 0.47 -0.2 2,548,175 - PGA1_c24430 0.47 -1.3 2,548,175 - PGA1_c24430 0.47 +0.2 2,548,266 - PGA1_c24430 0.60 -0.5 2,548,288 - PGA1_c24430 0.63 -0.1 2,548,290 + PGA1_c24430 0.63 +1.5 2,548,291 - PGA1_c24430 0.63 -0.2 2,548,298 - PGA1_c24430 0.64 -1.5 2,548,340 + PGA1_c24430 0.70 -0.8 2,548,348 - PGA1_c24430 0.71 -1.8 2,548,372 - PGA1_c24430 0.74 -1.5 2,548,413 + PGA1_c24430 0.80 -0.2 2,548,429 - PGA1_c24430 0.82 +1.0 2,548,436 - PGA1_c24430 0.83 +0.2 2,548,439 + PGA1_c24430 0.83 -0.8 2,548,447 - PGA1_c24430 0.85 -0.1 2,548,447 - PGA1_c24430 0.85 -0.8 2,548,503 - -1.2 2,548,533 - +0.1 2,548,535 - +0.1 2,548,556 + -0.1 2,548,612 - +0.8 2,548,626 + -0.4 2,548,634 - -1.3 2,548,702 + +0.5 2,548,707 + -0.6 2,548,759 + PGA1_c24440 0.20 +0.1 2,548,835 - PGA1_c24440 0.51 +1.7 2,548,856 + PGA1_c24440 0.59 -0.3 2,548,897 + PGA1_c24440 0.76 +0.7 2,548,898 + PGA1_c24440 0.76 +0.7 2,548,905 - PGA1_c24440 0.79 -0.1 2,548,905 - PGA1_c24440 0.79 +0.5 2,548,906 - PGA1_c24440 0.80 -1.3 2,548,908 + PGA1_c24440 0.80 +0.8 2,548,908 + PGA1_c24440 0.80 -1.0 2,549,042 - +0.1 2,549,154 - PGA1_c24450 0.29 -0.7 2,549,168 - PGA1_c24450 0.34 -2.4 2,549,229 - PGA1_c24450 0.55 +0.3 2,549,244 - PGA1_c24450 0.60 +1.1 2,549,276 + PGA1_c24450 0.70 -1.9 2,549,281 + PGA1_c24450 0.72 -2.1 2,549,333 - PGA1_c24450 0.90 -1.5 2,549,333 - PGA1_c24450 0.90 -0.9 2,549,358 + +0.5 2,549,366 - -1.1 2,549,476 - +0.2 2,549,532 + +3.5 2,549,589 + +1.5 2,549,597 - -1.3 2,549,611 + +0.6 2,549,635 + +2.0 2,549,635 + +0.2 2,549,695 + PGA1_c24460 0.20 -0.1 2,549,705 + PGA1_c24460 0.22 +0.5 2,549,705 + PGA1_c24460 0.22 -1.5 2,549,852 - PGA1_c24460 0.47 +0.5 2,549,880 - PGA1_c24460 0.52 -1.3 2,549,902 - PGA1_c24460 0.56 -0.7 2,549,919 + PGA1_c24460 0.59 +0.5 2,549,919 + PGA1_c24460 0.59 -0.8 2,549,919 + PGA1_c24460 0.59 +1.5 2,549,927 - PGA1_c24460 0.60 +0.1 2,549,927 - PGA1_c24460 0.60 -2.6 2,549,942 + PGA1_c24460 0.63 -1.8 2,549,942 + PGA1_c24460 0.63 +0.9 2,550,041 + PGA1_c24460 0.80 -0.5 2,550,335 - PGA1_c24470 0.26 +0.0
Or see this region's nucleotide sequence