Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21180 and PGA1_c21190 are separated by 300 nucleotides PGA1_c21190 and PGA1_c21200 are separated by 4 nucleotides
PGA1_c21180: PGA1_c21180 - thymidylate synthase ThyA, at 2,192,685 to 2,193,518
_c21180
PGA1_c21190: PGA1_c21190 - hypothetical protein, at 2,193,819 to 2,194,757
_c21190
PGA1_c21200: PGA1_c21200 - hypothetical protein, at 2,194,762 to 2,196,186
_c21200
Position (kb)
2193
2194
2195 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2193.614 kb on - strand at 2193.668 kb on - strand at 2193.668 kb on - strand at 2193.668 kb on - strand at 2193.687 kb on - strand at 2193.774 kb on + strand at 2193.840 kb on - strand at 2193.872 kb on - strand at 2193.945 kb on + strand, within PGA1_c21190 at 2194.082 kb on + strand, within PGA1_c21190 at 2194.090 kb on - strand, within PGA1_c21190 at 2194.090 kb on - strand at 2194.090 kb on - strand, within PGA1_c21190 at 2194.090 kb on - strand, within PGA1_c21190 at 2194.091 kb on - strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.095 kb on - strand, within PGA1_c21190 at 2194.205 kb on + strand, within PGA1_c21190 at 2194.288 kb on + strand, within PGA1_c21190 at 2194.293 kb on + strand, within PGA1_c21190 at 2194.368 kb on + strand, within PGA1_c21190 at 2194.390 kb on - strand, within PGA1_c21190 at 2194.490 kb on - strand, within PGA1_c21190 at 2194.520 kb on - strand, within PGA1_c21190 at 2194.520 kb on - strand, within PGA1_c21190 at 2194.643 kb on - strand, within PGA1_c21190 at 2194.658 kb on + strand, within PGA1_c21190 at 2194.743 kb on - strand at 2194.756 kb on + strand at 2194.838 kb on - strand at 2195.130 kb on + strand, within PGA1_c21200 at 2195.130 kb on + strand, within PGA1_c21200 at 2195.138 kb on - strand, within PGA1_c21200 at 2195.159 kb on + strand, within PGA1_c21200 at 2195.159 kb on + strand, within PGA1_c21200 at 2195.164 kb on + strand, within PGA1_c21200 at 2195.172 kb on - strand, within PGA1_c21200 at 2195.188 kb on + strand, within PGA1_c21200 at 2195.198 kb on + strand, within PGA1_c21200 at 2195.218 kb on + strand, within PGA1_c21200 at 2195.228 kb on + strand, within PGA1_c21200 at 2195.243 kb on - strand, within PGA1_c21200 at 2195.296 kb on - strand, within PGA1_c21200 at 2195.418 kb on - strand, within PGA1_c21200 at 2195.480 kb on - strand, within PGA1_c21200 at 2195.504 kb on + strand, within PGA1_c21200 at 2195.512 kb on + strand, within PGA1_c21200 at 2195.517 kb on + strand, within PGA1_c21200 at 2195.561 kb on + strand, within PGA1_c21200 at 2195.568 kb on - strand, within PGA1_c21200 at 2195.669 kb on - strand, within PGA1_c21200 at 2195.699 kb on - strand, within PGA1_c21200
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 2,193,614 - +1.4 2,193,668 - +0.3 2,193,668 - +0.2 2,193,668 - +1.1 2,193,687 - -0.1 2,193,774 + +1.1 2,193,840 - +1.4 2,193,872 - -1.3 2,193,945 + PGA1_c21190 0.13 +1.0 2,194,082 + PGA1_c21190 0.28 +0.3 2,194,090 - PGA1_c21190 0.29 -0.3 2,194,090 - -0.5 2,194,090 - PGA1_c21190 0.29 -0.5 2,194,090 - PGA1_c21190 0.29 +1.2 2,194,091 - PGA1_c21190 0.29 +1.5 2,194,092 + PGA1_c21190 0.29 -0.1 2,194,092 + PGA1_c21190 0.29 +1.3 2,194,092 + PGA1_c21190 0.29 +0.5 2,194,092 + PGA1_c21190 0.29 -1.1 2,194,092 + PGA1_c21190 0.29 +1.2 2,194,092 + PGA1_c21190 0.29 +1.7 2,194,095 - PGA1_c21190 0.29 +1.2 2,194,205 + PGA1_c21190 0.41 +0.5 2,194,288 + PGA1_c21190 0.50 -0.8 2,194,293 + PGA1_c21190 0.50 -1.3 2,194,368 + PGA1_c21190 0.58 +1.5 2,194,390 - PGA1_c21190 0.61 +1.5 2,194,490 - PGA1_c21190 0.71 -0.2 2,194,520 - PGA1_c21190 0.75 +1.7 2,194,520 - PGA1_c21190 0.75 +1.3 2,194,643 - PGA1_c21190 0.88 +0.8 2,194,658 + PGA1_c21190 0.89 +0.8 2,194,743 - -0.1 2,194,756 + +0.5 2,194,838 - +0.0 2,195,130 + PGA1_c21200 0.26 +0.4 2,195,130 + PGA1_c21200 0.26 +0.5 2,195,138 - PGA1_c21200 0.26 +2.5 2,195,159 + PGA1_c21200 0.28 +0.8 2,195,159 + PGA1_c21200 0.28 +1.0 2,195,164 + PGA1_c21200 0.28 +0.7 2,195,172 - PGA1_c21200 0.29 +2.0 2,195,188 + PGA1_c21200 0.30 +0.9 2,195,198 + PGA1_c21200 0.31 +0.5 2,195,218 + PGA1_c21200 0.32 +0.3 2,195,228 + PGA1_c21200 0.33 -0.6 2,195,243 - PGA1_c21200 0.34 +1.1 2,195,296 - PGA1_c21200 0.37 +1.5 2,195,418 - PGA1_c21200 0.46 +2.8 2,195,480 - PGA1_c21200 0.50 +0.4 2,195,504 + PGA1_c21200 0.52 +0.9 2,195,512 + PGA1_c21200 0.53 +1.2 2,195,517 + PGA1_c21200 0.53 -0.0 2,195,561 + PGA1_c21200 0.56 +0.3 2,195,568 - PGA1_c21200 0.57 +0.6 2,195,669 - PGA1_c21200 0.64 +0.5 2,195,699 - PGA1_c21200 0.66 +1.3
Or see this region's nucleotide sequence