Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16870 and PGA1_c16880 are separated by 198 nucleotides PGA1_c16880 and PGA1_c16890 overlap by 4 nucleotides PGA1_c16890 and PGA1_c16900 overlap by 4 nucleotides PGA1_c16900 and PGA1_c16910 are separated by 14 nucleotides
PGA1_c16870: PGA1_c16870 - Uncharacterized protein conserved in bacteria, at 1,761,186 to 1,762,859
_c16870
PGA1_c16880: PGA1_c16880 - anthranilate synthase component II, at 1,763,058 to 1,763,639
_c16880
PGA1_c16890: PGA1_c16890 - anthranilate phosphoribosyltransferase TrpD, at 1,763,636 to 1,764,664
_c16890
PGA1_c16900: PGA1_c16900 - uracil-DNA glycosylase Ung, at 1,764,661 to 1,765,314
_c16900
PGA1_c16910: PGA1_c16910 - indole-3-glycerol phosphate synthase TrpC, at 1,765,329 to 1,766,141
_c16910
Position (kb) 1763
1764
1765 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1762.717 kb on + strand at 1762.727 kb on + strand at 1762.859 kb on + strand at 1762.909 kb on - strand at 1762.911 kb on + strand at 1762.914 kb on - strand at 1762.916 kb on + strand at 1762.942 kb on - strand at 1763.012 kb on - strand at 1764.859 kb on - strand, within PGA1_c16900 at 1765.189 kb on + strand, within PGA1_c16900 at 1765.189 kb on + strand, within PGA1_c16900 at 1765.192 kb on - strand, within PGA1_c16900 at 1765.206 kb on + strand, within PGA1_c16900 at 1765.206 kb on + strand, within PGA1_c16900 at 1765.206 kb on + strand, within PGA1_c16900 at 1765.206 kb on + strand, within PGA1_c16900 at 1765.214 kb on - strand, within PGA1_c16900 at 1765.214 kb on - strand, within PGA1_c16900 at 1765.216 kb on - strand, within PGA1_c16900 at 1765.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 1,762,717 + +1.4 1,762,727 + +0.3 1,762,859 + -1.3 1,762,909 - -3.0 1,762,911 + -0.9 1,762,914 - -1.1 1,762,916 + -1.1 1,762,942 - -2.5 1,763,012 - -0.9 1,764,859 - PGA1_c16900 0.30 -2.6 1,765,189 + PGA1_c16900 0.81 -2.6 1,765,189 + PGA1_c16900 0.81 -1.6 1,765,192 - PGA1_c16900 0.81 -2.6 1,765,206 + PGA1_c16900 0.83 -2.3 1,765,206 + PGA1_c16900 0.83 -0.1 1,765,206 + PGA1_c16900 0.83 -2.6 1,765,206 + PGA1_c16900 0.83 -2.1 1,765,214 - PGA1_c16900 0.85 -2.5 1,765,214 - PGA1_c16900 0.85 -1.4 1,765,216 - PGA1_c16900 0.85 -1.4 1,765,290 + -1.7
Or see this region's nucleotide sequence