Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11830 and PGA1_c11840 are separated by 73 nucleotides PGA1_c11840 and PGA1_c11850 are separated by 92 nucleotides PGA1_c11850 and PGA1_c11860 are separated by 78 nucleotides PGA1_c11860 and PGA1_c11870 are separated by 79 nucleotides
PGA1_c11830: PGA1_c11830 - hypothetical protein, at 1,227,262 to 1,227,627
_c11830
PGA1_c11840: PGA1_c11840 - hypothetical protein, at 1,227,701 to 1,228,288
_c11840
PGA1_c11850: PGA1_c11850 - Phage shock protein A (IM30), suppresses sigma54-dependent transcription, at 1,228,381 to 1,229,058
_c11850
PGA1_c11860: PGA1_c11860 - Uncharacterized protein conserved in bacteria, at 1,229,137 to 1,229,421
_c11860
PGA1_c11870: PGA1_c11870 - serine hydroxymethyltransferase GlyA, at 1,229,501 to 1,230,799
_c11870
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1227.403 kb on - strand, within PGA1_c11830 at 1227.451 kb on + strand, within PGA1_c11830 at 1227.457 kb on - strand, within PGA1_c11830 at 1227.463 kb on + strand, within PGA1_c11830 at 1227.479 kb on + strand, within PGA1_c11830 at 1227.506 kb on + strand, within PGA1_c11830 at 1227.506 kb on - strand, within PGA1_c11830 at 1227.614 kb on - strand at 1227.645 kb on - strand at 1228.268 kb on + strand at 1228.429 kb on + strand at 1228.453 kb on - strand, within PGA1_c11850 at 1228.477 kb on - strand, within PGA1_c11850 at 1228.498 kb on - strand, within PGA1_c11850 at 1228.503 kb on - strand, within PGA1_c11850 at 1228.503 kb on - strand, within PGA1_c11850 at 1228.553 kb on + strand, within PGA1_c11850 at 1228.610 kb on - strand, within PGA1_c11850 at 1228.732 kb on - strand, within PGA1_c11850 at 1228.820 kb on - strand, within PGA1_c11850 at 1228.820 kb on - strand, within PGA1_c11850 at 1228.864 kb on - strand, within PGA1_c11850 at 1228.866 kb on + strand, within PGA1_c11850 at 1228.879 kb on - strand, within PGA1_c11850 at 1228.913 kb on + strand, within PGA1_c11850 at 1228.986 kb on - strand, within PGA1_c11850 at 1229.037 kb on - strand at 1229.134 kb on - strand at 1229.174 kb on + strand, within PGA1_c11860 at 1229.174 kb on + strand, within PGA1_c11860 at 1229.182 kb on - strand, within PGA1_c11860 at 1229.202 kb on - strand, within PGA1_c11860 at 1229.262 kb on + strand, within PGA1_c11860 at 1229.262 kb on + strand, within PGA1_c11860 at 1229.262 kb on + strand, within PGA1_c11860 at 1229.269 kb on + strand, within PGA1_c11860 at 1229.270 kb on - strand, within PGA1_c11860 at 1229.270 kb on - strand, within PGA1_c11860 at 1229.291 kb on + strand, within PGA1_c11860 at 1229.296 kb on + strand, within PGA1_c11860 at 1229.299 kb on - strand, within PGA1_c11860 at 1229.299 kb on - strand, within PGA1_c11860 at 1229.299 kb on - strand, within PGA1_c11860 at 1229.301 kb on + strand, within PGA1_c11860 at 1229.301 kb on + strand, within PGA1_c11860 at 1229.301 kb on + strand, within PGA1_c11860 at 1229.309 kb on - strand, within PGA1_c11860 at 1229.400 kb on + strand at 1229.408 kb on - strand at 1229.409 kb on - strand at 1229.415 kb on + strand at 1229.452 kb on - strand at 1229.506 kb on - strand at 1229.507 kb on - strand at 1229.561 kb on + strand at 1229.582 kb on - strand at 1229.674 kb on - strand, within PGA1_c11870 at 1229.682 kb on - strand, within PGA1_c11870 at 1229.692 kb on - strand, within PGA1_c11870 at 1229.765 kb on + strand, within PGA1_c11870 at 1229.765 kb on + strand, within PGA1_c11870 at 1229.765 kb on + strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.773 kb on - strand, within PGA1_c11870 at 1229.792 kb on + strand, within PGA1_c11870 at 1229.792 kb on + strand, within PGA1_c11870 at 1229.807 kb on - strand, within PGA1_c11870 at 1229.829 kb on - strand, within PGA1_c11870 at 1229.865 kb on + strand, within PGA1_c11870 at 1229.873 kb on - strand, within PGA1_c11870 at 1229.981 kb on + strand, within PGA1_c11870 at 1229.999 kb on - strand, within PGA1_c11870 at 1230.001 kb on - strand, within PGA1_c11870
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 1,227,403 - PGA1_c11830 0.39 +0.8 1,227,451 + PGA1_c11830 0.52 -0.3 1,227,457 - PGA1_c11830 0.53 +0.5 1,227,463 + PGA1_c11830 0.55 -2.5 1,227,479 + PGA1_c11830 0.59 -0.8 1,227,506 + PGA1_c11830 0.67 +0.2 1,227,506 - PGA1_c11830 0.67 +1.7 1,227,614 - +0.8 1,227,645 - +1.2 1,228,268 + +0.9 1,228,429 + -0.8 1,228,453 - PGA1_c11850 0.11 +1.1 1,228,477 - PGA1_c11850 0.14 -1.2 1,228,498 - PGA1_c11850 0.17 -0.5 1,228,503 - PGA1_c11850 0.18 +0.1 1,228,503 - PGA1_c11850 0.18 +0.6 1,228,553 + PGA1_c11850 0.25 -0.5 1,228,610 - PGA1_c11850 0.34 -1.5 1,228,732 - PGA1_c11850 0.52 +0.0 1,228,820 - PGA1_c11850 0.65 +0.6 1,228,820 - PGA1_c11850 0.65 -0.2 1,228,864 - PGA1_c11850 0.71 -0.2 1,228,866 + PGA1_c11850 0.72 -0.7 1,228,879 - PGA1_c11850 0.73 -0.7 1,228,913 + PGA1_c11850 0.78 -0.8 1,228,986 - PGA1_c11850 0.89 -0.1 1,229,037 - -0.5 1,229,134 - +0.7 1,229,174 + PGA1_c11860 0.13 +0.1 1,229,174 + PGA1_c11860 0.13 -2.1 1,229,182 - PGA1_c11860 0.16 +0.8 1,229,202 - PGA1_c11860 0.23 +0.2 1,229,262 + PGA1_c11860 0.44 +1.5 1,229,262 + PGA1_c11860 0.44 -0.1 1,229,262 + PGA1_c11860 0.44 -0.9 1,229,269 + PGA1_c11860 0.46 +0.7 1,229,270 - PGA1_c11860 0.47 +0.9 1,229,270 - PGA1_c11860 0.47 +0.6 1,229,291 + PGA1_c11860 0.54 -0.5 1,229,296 + PGA1_c11860 0.56 +1.5 1,229,299 - PGA1_c11860 0.57 -0.9 1,229,299 - PGA1_c11860 0.57 -1.4 1,229,299 - PGA1_c11860 0.57 +0.1 1,229,301 + PGA1_c11860 0.58 +3.8 1,229,301 + PGA1_c11860 0.58 -0.7 1,229,301 + PGA1_c11860 0.58 +1.5 1,229,309 - PGA1_c11860 0.60 +1.1 1,229,400 + -0.0 1,229,408 - +0.6 1,229,409 - +0.4 1,229,415 + -0.7 1,229,452 - +1.8 1,229,506 - -1.7 1,229,507 - -1.8 1,229,561 + -2.4 1,229,582 - -1.3 1,229,674 - PGA1_c11870 0.13 -2.5 1,229,682 - PGA1_c11870 0.14 -2.1 1,229,692 - PGA1_c11870 0.15 -3.0 1,229,765 + PGA1_c11870 0.20 -0.8 1,229,765 + PGA1_c11870 0.20 +0.5 1,229,765 + PGA1_c11870 0.20 +0.5 1,229,773 - PGA1_c11870 0.21 -2.1 1,229,773 - PGA1_c11870 0.21 -0.1 1,229,773 - PGA1_c11870 0.21 -1.7 1,229,773 - PGA1_c11870 0.21 -2.1 1,229,773 - PGA1_c11870 0.21 -1.5 1,229,773 - PGA1_c11870 0.21 -3.3 1,229,773 - PGA1_c11870 0.21 -0.1 1,229,773 - PGA1_c11870 0.21 -1.5 1,229,792 + PGA1_c11870 0.22 -1.7 1,229,792 + PGA1_c11870 0.22 -1.9 1,229,807 - PGA1_c11870 0.24 -3.4 1,229,829 - PGA1_c11870 0.25 -1.1 1,229,865 + PGA1_c11870 0.28 -1.8 1,229,873 - PGA1_c11870 0.29 -2.1 1,229,981 + PGA1_c11870 0.37 -0.8 1,229,999 - PGA1_c11870 0.38 -1.1 1,230,001 - PGA1_c11870 0.38 -3.5
Or see this region's nucleotide sequence