Experiment: No vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00950 and PGA1_c00960 overlap by 4 nucleotides PGA1_c00960 and PGA1_c00970 are separated by 121 nucleotides PGA1_c00970 and PGA1_c00980 are separated by 190 nucleotides PGA1_c00980 and PGA1_c00990 are separated by 139 nucleotides
PGA1_c00950: PGA1_c00950 - Protein of unknown function (DUF3429)., at 90,668 to 91,120
_c00950
PGA1_c00960: PGA1_c00960 - lysE type translocator-like protein, at 91,117 to 91,734
_c00960
PGA1_c00970: PGA1_c00970 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase IspH, at 91,856 to 92,806
_c00970
PGA1_c00980: PGA1_c00980 - Uncharacterized conserved protein, at 92,997 to 93,566
_c00980
PGA1_c00990: PGA1_c00990 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase FolK, at 93,706 to 94,275
_c00990
Position (kb) 91
92
93 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 90.878 kb on + strand, within PGA1_c00950 at 90.878 kb on + strand, within PGA1_c00950 at 90.879 kb on + strand, within PGA1_c00950 at 90.963 kb on + strand, within PGA1_c00950 at 90.971 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.032 kb on + strand, within PGA1_c00950 at 91.040 kb on - strand, within PGA1_c00950 at 91.051 kb on + strand, within PGA1_c00950 at 91.056 kb on + strand, within PGA1_c00950 at 91.077 kb on - strand at 91.077 kb on - strand at 91.079 kb on + strand at 91.123 kb on - strand at 91.238 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.284 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.402 kb on - strand, within PGA1_c00960 at 91.523 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.538 kb on + strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.632 kb on - strand, within PGA1_c00960 at 91.639 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.656 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.677 kb on - strand at 91.693 kb on + strand at 91.719 kb on - strand at 91.758 kb on + strand at 91.758 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.794 kb on + strand at 91.820 kb on + strand at 91.830 kb on - strand at 91.830 kb on - strand at 91.847 kb on - strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.851 kb on + strand at 92.856 kb on + strand at 92.859 kb on - strand at 92.859 kb on - strand at 92.859 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.864 kb on - strand at 92.877 kb on + strand at 92.947 kb on + strand at 92.951 kb on + strand at 92.959 kb on - strand at 93.055 kb on + strand, within PGA1_c00980 at 93.069 kb on - strand, within PGA1_c00980 at 93.141 kb on + strand, within PGA1_c00980 at 93.149 kb on + strand, within PGA1_c00980 at 93.157 kb on - strand, within PGA1_c00980 at 93.164 kb on + strand, within PGA1_c00980 at 93.248 kb on + strand, within PGA1_c00980 at 93.255 kb on + strand, within PGA1_c00980 at 93.256 kb on - strand, within PGA1_c00980 at 93.256 kb on - strand, within PGA1_c00980 at 93.285 kb on + strand, within PGA1_c00980 at 93.285 kb on + strand, within PGA1_c00980 at 93.324 kb on - strand, within PGA1_c00980 at 93.341 kb on + strand, within PGA1_c00980 at 93.345 kb on + strand, within PGA1_c00980 at 93.362 kb on - strand, within PGA1_c00980 at 93.413 kb on + strand, within PGA1_c00980 at 93.418 kb on + strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.421 kb on - strand, within PGA1_c00980 at 93.422 kb on + strand, within PGA1_c00980 at 93.426 kb on - strand, within PGA1_c00980 at 93.430 kb on - strand, within PGA1_c00980 at 93.439 kb on + strand, within PGA1_c00980 at 93.466 kb on - strand, within PGA1_c00980 at 93.494 kb on - strand, within PGA1_c00980 at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.511 kb on + strand at 93.518 kb on + strand at 93.529 kb on + strand at 93.529 kb on + strand at 93.539 kb on + strand at 93.547 kb on + strand at 93.547 kb on - strand at 93.547 kb on - strand at 93.590 kb on + strand at 93.597 kb on + strand at 93.598 kb on - strand at 93.608 kb on - strand at 93.643 kb on + strand at 93.652 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t1 remove 90,878 + PGA1_c00950 0.46 -0.0 90,878 + PGA1_c00950 0.46 -0.4 90,879 + PGA1_c00950 0.47 -2.4 90,963 + PGA1_c00950 0.65 -0.0 90,971 - PGA1_c00950 0.67 +0.4 91,025 - PGA1_c00950 0.79 -0.9 91,025 - PGA1_c00950 0.79 +0.5 91,032 + PGA1_c00950 0.80 +1.3 91,040 - PGA1_c00950 0.82 +0.4 91,051 + PGA1_c00950 0.85 +1.5 91,056 + PGA1_c00950 0.86 +1.2 91,077 - +0.3 91,077 - -0.2 91,079 + -0.9 91,123 - -0.0 91,238 + PGA1_c00960 0.20 +1.3 91,264 + PGA1_c00960 0.24 +0.7 91,264 + PGA1_c00960 0.24 -0.6 91,264 + PGA1_c00960 0.24 -1.5 91,264 + PGA1_c00960 0.24 +0.5 91,264 + PGA1_c00960 0.24 -0.3 91,272 - PGA1_c00960 0.25 +0.1 91,272 - PGA1_c00960 0.25 +0.2 91,272 - PGA1_c00960 0.25 +0.6 91,272 - PGA1_c00960 0.25 -0.5 91,272 - PGA1_c00960 0.25 -0.6 91,272 - PGA1_c00960 0.25 -1.0 91,272 - PGA1_c00960 0.25 -0.5 91,272 - PGA1_c00960 0.25 -1.5 91,272 - PGA1_c00960 0.25 -0.0 91,272 - PGA1_c00960 0.25 -1.0 91,284 + PGA1_c00960 0.27 -1.5 91,368 + PGA1_c00960 0.41 +0.1 91,368 + PGA1_c00960 0.41 -0.3 91,402 - PGA1_c00960 0.46 -0.0 91,523 + PGA1_c00960 0.66 +0.1 91,536 + PGA1_c00960 0.68 +0.1 91,536 + PGA1_c00960 0.68 +0.7 91,536 + PGA1_c00960 0.68 -0.0 91,536 + PGA1_c00960 0.68 -0.8 91,536 + PGA1_c00960 0.68 +1.3 91,536 + PGA1_c00960 0.68 +0.5 91,536 + PGA1_c00960 0.68 -0.8 91,536 + PGA1_c00960 0.68 +0.1 91,536 + PGA1_c00960 0.68 -0.2 91,538 + PGA1_c00960 0.68 +2.5 91,544 - PGA1_c00960 0.69 +0.3 91,544 - PGA1_c00960 0.69 +0.7 91,544 - PGA1_c00960 0.69 +1.1 91,544 - PGA1_c00960 0.69 -1.0 91,544 - PGA1_c00960 0.69 -0.8 91,551 - PGA1_c00960 0.70 +1.3 91,551 - PGA1_c00960 0.70 -0.5 91,554 - PGA1_c00960 0.71 +0.3 91,554 - PGA1_c00960 0.71 +0.3 91,632 - PGA1_c00960 0.83 +0.2 91,639 + PGA1_c00960 0.84 +0.5 91,644 + PGA1_c00960 0.85 -1.6 91,644 + PGA1_c00960 0.85 -0.2 91,656 + PGA1_c00960 0.87 +1.0 91,664 + PGA1_c00960 0.89 -0.0 91,664 + PGA1_c00960 0.89 +1.0 91,674 + +2.5 91,674 + +0.3 91,674 + -0.0 91,674 + +0.1 91,677 - +1.0 91,693 + +1.0 91,719 - -2.4 91,758 + +1.2 91,758 + +1.0 91,768 + -1.1 91,768 + -0.0 91,768 + +3.1 91,768 + -0.2 91,768 + +0.7 91,768 + +0.5 91,768 + +1.8 91,768 + -0.5 91,776 - -0.7 91,776 - -0.3 91,776 - +0.9 91,776 - +0.7 91,776 - -0.5 91,776 - -0.5 91,776 - -0.3 91,776 - +0.5 91,776 - -0.3 91,776 - -2.0 91,776 - +0.4 91,776 - +2.0 91,776 - +0.4 91,776 - -0.3 91,776 - +1.1 91,794 + -0.0 91,820 + -1.4 91,830 - -0.0 91,830 - -0.0 91,847 - -0.3 92,851 + +0.8 92,851 + -2.0 92,851 + -1.1 92,851 + +1.0 92,851 + -0.8 92,856 + +1.5 92,859 - -1.2 92,859 - +0.5 92,859 - -0.3 92,864 - -1.3 92,864 - -1.6 92,864 - -1.6 92,864 - +0.2 92,864 - +0.7 92,877 + +0.3 92,947 + +1.5 92,951 + -1.3 92,959 - -1.5 93,055 + PGA1_c00980 0.10 +1.0 93,069 - PGA1_c00980 0.13 -1.3 93,141 + PGA1_c00980 0.25 -0.8 93,149 + PGA1_c00980 0.27 -1.8 93,157 - PGA1_c00980 0.28 -3.9 93,164 + PGA1_c00980 0.29 -0.9 93,248 + PGA1_c00980 0.44 -1.3 93,255 + PGA1_c00980 0.45 -1.0 93,256 - PGA1_c00980 0.45 -0.0 93,256 - PGA1_c00980 0.45 -0.8 93,285 + PGA1_c00980 0.51 -1.3 93,285 + PGA1_c00980 0.51 -1.6 93,324 - PGA1_c00980 0.57 -1.5 93,341 + PGA1_c00980 0.60 -1.6 93,345 + PGA1_c00980 0.61 -1.8 93,362 - PGA1_c00980 0.64 -1.0 93,413 + PGA1_c00980 0.73 -0.8 93,418 + PGA1_c00980 0.74 -2.2 93,421 - PGA1_c00980 0.74 -1.6 93,421 - PGA1_c00980 0.74 -2.2 93,422 + PGA1_c00980 0.75 -1.5 93,426 - PGA1_c00980 0.75 -2.0 93,430 - PGA1_c00980 0.76 +1.5 93,439 + PGA1_c00980 0.78 -1.0 93,466 - PGA1_c00980 0.82 -0.9 93,494 - PGA1_c00980 0.87 -1.3 93,511 + -1.6 93,511 + -1.5 93,511 + +0.5 93,511 + -1.9 93,511 + -0.0 93,518 + -2.4 93,529 + -0.9 93,529 + -0.0 93,539 + -1.3 93,547 + -1.6 93,547 - -1.2 93,547 - -0.0 93,590 + -1.5 93,597 + -1.6 93,598 - -1.9 93,608 - -1.5 93,643 + -1.9 93,652 - -1.3
Or see this region's nucleotide sequence