Experiment: No vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05110 and PGA1_c05120 overlap by 10 nucleotides PGA1_c05120 and PGA1_c05130 overlap by 1 nucleotides PGA1_c05130 and PGA1_c05140 are separated by 53 nucleotides
PGA1_c05110: PGA1_c05110 - dihydrodipicolinate synthase domain-containing protein, at 503,564 to 504,523
_c05110
PGA1_c05120: PGA1_c05120 - 4-hydroxyproline epimerase, at 504,514 to 505,458
_c05120
PGA1_c05130: PGA1_c05130 - aldehyde dehydrogenase, at 505,458 to 506,888
_c05130
PGA1_c05140: PGA1_c05140 - D-amino acid dehydrogenase small subunit, at 506,942 to 508,192
_c05140
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 504.471 kb on - strand at 504.474 kb on + strand at 504.474 kb on + strand at 504.482 kb on + strand at 504.490 kb on - strand at 504.490 kb on - strand at 504.554 kb on + strand at 504.562 kb on - strand at 504.644 kb on + strand, within PGA1_c05120 at 504.652 kb on - strand, within PGA1_c05120 at 504.652 kb on - strand, within PGA1_c05120 at 504.717 kb on - strand, within PGA1_c05120 at 504.787 kb on - strand, within PGA1_c05120 at 504.805 kb on + strand, within PGA1_c05120 at 504.813 kb on - strand, within PGA1_c05120 at 504.815 kb on + strand, within PGA1_c05120 at 504.857 kb on + strand, within PGA1_c05120 at 504.983 kb on - strand, within PGA1_c05120 at 505.004 kb on + strand, within PGA1_c05120 at 505.010 kb on - strand, within PGA1_c05120 at 505.012 kb on - strand, within PGA1_c05120 at 505.022 kb on + strand, within PGA1_c05120 at 505.029 kb on - strand, within PGA1_c05120 at 505.032 kb on + strand, within PGA1_c05120 at 505.073 kb on + strand, within PGA1_c05120 at 505.075 kb on - strand, within PGA1_c05120 at 505.078 kb on + strand, within PGA1_c05120 at 505.085 kb on - strand, within PGA1_c05120 at 505.085 kb on - strand, within PGA1_c05120 at 505.101 kb on - strand, within PGA1_c05120 at 505.118 kb on + strand, within PGA1_c05120 at 505.142 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.147 kb on + strand, within PGA1_c05120 at 505.150 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.155 kb on - strand, within PGA1_c05120 at 505.198 kb on + strand, within PGA1_c05120 at 505.198 kb on + strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.206 kb on - strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.286 kb on + strand, within PGA1_c05120 at 505.313 kb on + strand, within PGA1_c05120 at 505.331 kb on + strand, within PGA1_c05120 at 505.420 kb on - strand at 505.442 kb on + strand at 505.447 kb on + strand at 505.450 kb on - strand at 505.497 kb on - strand at 505.497 kb on - strand at 505.498 kb on - strand at 505.550 kb on - strand at 505.611 kb on + strand, within PGA1_c05130 at 505.613 kb on + strand, within PGA1_c05130 at 505.619 kb on - strand, within PGA1_c05130 at 505.619 kb on - strand, within PGA1_c05130 at 505.645 kb on + strand, within PGA1_c05130 at 505.655 kb on + strand, within PGA1_c05130 at 505.698 kb on - strand, within PGA1_c05130 at 505.698 kb on - strand, within PGA1_c05130 at 505.724 kb on + strand, within PGA1_c05130 at 505.724 kb on + strand, within PGA1_c05130 at 505.732 kb on - strand, within PGA1_c05130 at 505.732 kb on - strand, within PGA1_c05130 at 505.792 kb on + strand, within PGA1_c05130 at 505.813 kb on + strand, within PGA1_c05130 at 505.830 kb on + strand, within PGA1_c05130 at 505.838 kb on - strand, within PGA1_c05130 at 505.881 kb on + strand, within PGA1_c05130 at 505.938 kb on + strand, within PGA1_c05130 at 505.938 kb on + strand, within PGA1_c05130 at 505.943 kb on + strand, within PGA1_c05130 at 505.943 kb on + strand, within PGA1_c05130 at 505.946 kb on - strand, within PGA1_c05130 at 505.946 kb on - strand, within PGA1_c05130 at 506.074 kb on + strand, within PGA1_c05130 at 506.077 kb on - strand, within PGA1_c05130 at 506.088 kb on - strand, within PGA1_c05130 at 506.106 kb on - strand, within PGA1_c05130 at 506.187 kb on + strand, within PGA1_c05130 at 506.189 kb on - strand, within PGA1_c05130 at 506.235 kb on + strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.243 kb on - strand, within PGA1_c05130 at 506.286 kb on + strand, within PGA1_c05130 at 506.286 kb on + strand, within PGA1_c05130 at 506.291 kb on + strand, within PGA1_c05130 at 506.294 kb on - strand, within PGA1_c05130 at 506.294 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.299 kb on - strand, within PGA1_c05130 at 506.300 kb on - strand, within PGA1_c05130 at 506.374 kb on - strand, within PGA1_c05130 at 506.391 kb on + strand, within PGA1_c05130 at 506.416 kb on + strand, within PGA1_c05130 at 506.497 kb on - strand, within PGA1_c05130 at 506.508 kb on + strand, within PGA1_c05130 at 506.516 kb on - strand, within PGA1_c05130 at 506.518 kb on + strand, within PGA1_c05130 at 506.526 kb on - strand, within PGA1_c05130 at 506.526 kb on - strand, within PGA1_c05130 at 506.535 kb on - strand, within PGA1_c05130 at 506.684 kb on + strand, within PGA1_c05130 at 506.797 kb on + strand at 506.829 kb on - strand at 506.871 kb on + strand at 506.872 kb on + strand at 506.962 kb on - strand at 506.962 kb on - strand at 507.093 kb on - strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.193 kb on + strand, within PGA1_c05140 at 507.201 kb on - strand, within PGA1_c05140 at 507.263 kb on - strand, within PGA1_c05140 at 507.281 kb on - strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.457 kb on + strand, within PGA1_c05140 at 507.465 kb on - strand, within PGA1_c05140 at 507.518 kb on + strand, within PGA1_c05140 at 507.525 kb on + strand, within PGA1_c05140 at 507.528 kb on - strand, within PGA1_c05140 at 507.528 kb on - strand, within PGA1_c05140 at 507.533 kb on - strand, within PGA1_c05140 at 507.602 kb on + strand, within PGA1_c05140 at 507.620 kb on - strand, within PGA1_c05140 at 507.647 kb on + strand, within PGA1_c05140 at 507.656 kb on + strand, within PGA1_c05140 at 507.727 kb on + strand, within PGA1_c05140 at 507.734 kb on + strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.806 kb on - strand, within PGA1_c05140 at 507.815 kb on + strand, within PGA1_c05140 at 507.815 kb on + strand, within PGA1_c05140 at 507.869 kb on - strand, within PGA1_c05140 at 507.871 kb on + strand, within PGA1_c05140
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t1 remove 504,471 - +0.8 504,474 + +0.4 504,474 + -1.0 504,482 + +0.1 504,490 - +0.8 504,490 - +0.5 504,554 + -1.3 504,562 - -1.6 504,644 + PGA1_c05120 0.14 +0.4 504,652 - PGA1_c05120 0.15 -1.0 504,652 - PGA1_c05120 0.15 +0.2 504,717 - PGA1_c05120 0.21 -1.3 504,787 - PGA1_c05120 0.29 +1.8 504,805 + PGA1_c05120 0.31 +1.4 504,813 - PGA1_c05120 0.32 +0.5 504,815 + PGA1_c05120 0.32 +2.3 504,857 + PGA1_c05120 0.36 -0.0 504,983 - PGA1_c05120 0.50 +0.4 505,004 + PGA1_c05120 0.52 -0.4 505,010 - PGA1_c05120 0.52 -0.0 505,012 - PGA1_c05120 0.53 +0.5 505,022 + PGA1_c05120 0.54 +1.0 505,029 - PGA1_c05120 0.54 +0.2 505,032 + PGA1_c05120 0.55 +0.7 505,073 + PGA1_c05120 0.59 -0.8 505,075 - PGA1_c05120 0.59 -1.3 505,078 + PGA1_c05120 0.60 -0.5 505,085 - PGA1_c05120 0.60 +1.5 505,085 - PGA1_c05120 0.60 -0.8 505,101 - PGA1_c05120 0.62 +0.1 505,118 + PGA1_c05120 0.64 -0.3 505,142 + PGA1_c05120 0.66 +0.8 505,147 + PGA1_c05120 0.67 +0.4 505,147 + PGA1_c05120 0.67 +1.2 505,147 + PGA1_c05120 0.67 -1.7 505,147 + PGA1_c05120 0.67 -0.4 505,150 - PGA1_c05120 0.67 +1.1 505,155 - PGA1_c05120 0.68 +1.5 505,155 - PGA1_c05120 0.68 -0.2 505,155 - PGA1_c05120 0.68 -1.3 505,155 - PGA1_c05120 0.68 -0.0 505,155 - PGA1_c05120 0.68 -0.7 505,155 - PGA1_c05120 0.68 +1.1 505,155 - PGA1_c05120 0.68 -0.8 505,198 + PGA1_c05120 0.72 -1.6 505,198 + PGA1_c05120 0.72 -1.2 505,206 - PGA1_c05120 0.73 +0.5 505,206 - PGA1_c05120 0.73 +0.5 505,206 - PGA1_c05120 0.73 -0.2 505,206 - PGA1_c05120 0.73 -1.4 505,206 - PGA1_c05120 0.73 +0.4 505,286 + PGA1_c05120 0.82 +0.2 505,286 + PGA1_c05120 0.82 -1.0 505,286 + PGA1_c05120 0.82 +0.7 505,313 + PGA1_c05120 0.85 -2.9 505,331 + PGA1_c05120 0.86 +0.3 505,420 - -1.1 505,442 + -1.9 505,447 + -0.9 505,450 - +0.2 505,497 - -1.2 505,497 - +0.1 505,498 - +0.0 505,550 - +1.1 505,611 + PGA1_c05130 0.11 +0.8 505,613 + PGA1_c05130 0.11 +1.5 505,619 - PGA1_c05130 0.11 +1.7 505,619 - PGA1_c05130 0.11 -0.8 505,645 + PGA1_c05130 0.13 +1.2 505,655 + PGA1_c05130 0.14 +0.4 505,698 - PGA1_c05130 0.17 -1.5 505,698 - PGA1_c05130 0.17 +0.8 505,724 + PGA1_c05130 0.19 -0.7 505,724 + PGA1_c05130 0.19 +0.4 505,732 - PGA1_c05130 0.19 +0.2 505,732 - PGA1_c05130 0.19 -0.2 505,792 + PGA1_c05130 0.23 -1.1 505,813 + PGA1_c05130 0.25 -0.3 505,830 + PGA1_c05130 0.26 +0.2 505,838 - PGA1_c05130 0.27 +0.3 505,881 + PGA1_c05130 0.30 +1.5 505,938 + PGA1_c05130 0.34 +1.1 505,938 + PGA1_c05130 0.34 +1.5 505,943 + PGA1_c05130 0.34 -1.0 505,943 + PGA1_c05130 0.34 +0.1 505,946 - PGA1_c05130 0.34 -1.7 505,946 - PGA1_c05130 0.34 -0.3 506,074 + PGA1_c05130 0.43 +0.5 506,077 - PGA1_c05130 0.43 +0.3 506,088 - PGA1_c05130 0.44 +0.3 506,106 - PGA1_c05130 0.45 -0.3 506,187 + PGA1_c05130 0.51 +0.5 506,189 - PGA1_c05130 0.51 -1.6 506,235 + PGA1_c05130 0.54 +0.2 506,243 - PGA1_c05130 0.55 -1.5 506,243 - PGA1_c05130 0.55 -0.7 506,243 - PGA1_c05130 0.55 +0.8 506,286 + PGA1_c05130 0.58 +0.8 506,286 + PGA1_c05130 0.58 +1.1 506,291 + PGA1_c05130 0.58 -2.6 506,294 - PGA1_c05130 0.58 -0.5 506,294 - PGA1_c05130 0.58 +1.5 506,299 - PGA1_c05130 0.59 -0.0 506,299 - PGA1_c05130 0.59 +1.1 506,299 - PGA1_c05130 0.59 +0.3 506,299 - PGA1_c05130 0.59 +0.7 506,299 - PGA1_c05130 0.59 +1.5 506,300 - PGA1_c05130 0.59 +2.5 506,374 - PGA1_c05130 0.64 -0.9 506,391 + PGA1_c05130 0.65 +0.4 506,416 + PGA1_c05130 0.67 -0.8 506,497 - PGA1_c05130 0.73 -1.2 506,508 + PGA1_c05130 0.73 +1.1 506,516 - PGA1_c05130 0.74 +0.4 506,518 + PGA1_c05130 0.74 -0.3 506,526 - PGA1_c05130 0.75 -1.0 506,526 - PGA1_c05130 0.75 +0.7 506,535 - PGA1_c05130 0.75 -1.0 506,684 + PGA1_c05130 0.86 -1.9 506,797 + -0.8 506,829 - +1.0 506,871 + +1.3 506,872 + -0.2 506,962 - -0.6 506,962 - +0.5 507,093 - PGA1_c05140 0.12 -1.9 507,193 + PGA1_c05140 0.20 +0.2 507,193 + PGA1_c05140 0.20 -0.3 507,193 + PGA1_c05140 0.20 -0.0 507,201 - PGA1_c05140 0.21 +1.0 507,263 - PGA1_c05140 0.26 +0.1 507,281 - PGA1_c05140 0.27 -1.6 507,457 + PGA1_c05140 0.41 -1.8 507,457 + PGA1_c05140 0.41 -0.3 507,457 + PGA1_c05140 0.41 -0.5 507,465 - PGA1_c05140 0.42 +1.5 507,518 + PGA1_c05140 0.46 -2.6 507,525 + PGA1_c05140 0.47 -0.9 507,528 - PGA1_c05140 0.47 -1.0 507,528 - PGA1_c05140 0.47 +1.3 507,533 - PGA1_c05140 0.47 +0.1 507,602 + PGA1_c05140 0.53 +0.5 507,620 - PGA1_c05140 0.54 -0.0 507,647 + PGA1_c05140 0.56 +0.5 507,656 + PGA1_c05140 0.57 +2.0 507,727 + PGA1_c05140 0.63 -0.6 507,734 + PGA1_c05140 0.63 -0.5 507,806 - PGA1_c05140 0.69 +0.5 507,806 - PGA1_c05140 0.69 +1.0 507,806 - PGA1_c05140 0.69 +0.2 507,815 + PGA1_c05140 0.70 -0.4 507,815 + PGA1_c05140 0.70 -0.1 507,869 - PGA1_c05140 0.74 +1.5 507,871 + PGA1_c05140 0.74 +1.0
Or see this region's nucleotide sequence