Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25590 and BWI76_RS25595 are separated by 226 nucleotides BWI76_RS25595 and BWI76_RS25600 are separated by 39 nucleotides BWI76_RS25600 and BWI76_RS25605 are separated by 14 nucleotides BWI76_RS25605 and BWI76_RS25610 are separated by 56 nucleotides BWI76_RS25610 and BWI76_RS25615 are separated by 181 nucleotides BWI76_RS25615 and BWI76_RS25620 are separated by 77 nucleotides
BWI76_RS25590: BWI76_RS25590 - membrane protein, at 5,319,713 to 5,319,934
_RS25590
BWI76_RS25595: BWI76_RS25595 - 5S ribosomal RNA, at 5,320,161 to 5,320,276
_RS25595
BWI76_RS25600: BWI76_RS25600 - tRNA-Thr, at 5,320,316 to 5,320,391
_RS25600
BWI76_RS25605: BWI76_RS25605 - 5S ribosomal RNA, at 5,320,406 to 5,320,521
_RS25605
BWI76_RS25610: BWI76_RS25610 - 23S ribosomal RNA, at 5,320,578 to 5,323,505
_RS25610
BWI76_RS25615: BWI76_RS25615 - tRNA-Glu, at 5,323,687 to 5,323,761
_RS25615
BWI76_RS25620: BWI76_RS25620 - 16S ribosomal RNA, at 5,323,839 to 5,325,392
_RS25620
Position (kb) 5320
5321
5322
5323
5324 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5319.629 kb on + strand at 5319.634 kb on - strand at 5319.637 kb on - strand at 5319.639 kb on - strand at 5319.656 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.700 kb on - strand at 5319.742 kb on + strand, within BWI76_RS25590 at 5319.754 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.772 kb on - strand, within BWI76_RS25590 at 5319.774 kb on + strand, within BWI76_RS25590 at 5319.782 kb on - strand, within BWI76_RS25590 at 5319.812 kb on + strand, within BWI76_RS25590 at 5319.817 kb on + strand, within BWI76_RS25590 at 5319.817 kb on + strand, within BWI76_RS25590 at 5319.826 kb on - strand, within BWI76_RS25590 at 5319.850 kb on - strand, within BWI76_RS25590 at 5319.995 kb on + strand at 5320.004 kb on - strand at 5320.019 kb on - strand at 5320.353 kb on - strand, within BWI76_RS25600
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 5,319,629 + +0.2 5,319,634 - -0.5 5,319,637 - -2.0 5,319,639 - -0.3 5,319,656 + -0.4 5,319,664 + +0.4 5,319,664 + +0.6 5,319,664 + -0.1 5,319,664 + +0.4 5,319,664 + +0.7 5,319,664 + -0.1 5,319,664 + -0.5 5,319,664 + -0.1 5,319,672 - +0.1 5,319,672 - +0.6 5,319,672 - -0.3 5,319,672 - -0.4 5,319,672 - +0.2 5,319,672 - -0.8 5,319,672 - +2.6 5,319,672 - -0.1 5,319,672 - +0.4 5,319,672 - +0.7 5,319,672 - +0.2 5,319,675 + -0.5 5,319,675 + +0.9 5,319,675 + -0.2 5,319,675 + +0.2 5,319,675 + -0.4 5,319,675 + -0.6 5,319,675 + -0.4 5,319,675 + +0.3 5,319,683 - +0.7 5,319,683 - +0.2 5,319,683 - +0.0 5,319,683 - +0.0 5,319,683 - -0.1 5,319,683 - +0.0 5,319,683 - +0.2 5,319,700 - +0.4 5,319,742 + BWI76_RS25590 0.13 -0.6 5,319,754 + BWI76_RS25590 0.18 +0.4 5,319,764 + BWI76_RS25590 0.23 +0.5 5,319,764 + BWI76_RS25590 0.23 -0.2 5,319,764 + BWI76_RS25590 0.23 -0.2 5,319,764 + BWI76_RS25590 0.23 +0.6 5,319,772 - BWI76_RS25590 0.27 -0.5 5,319,774 + BWI76_RS25590 0.27 -0.1 5,319,782 - BWI76_RS25590 0.31 +2.2 5,319,812 + BWI76_RS25590 0.45 +0.2 5,319,817 + BWI76_RS25590 0.47 +0.8 5,319,817 + BWI76_RS25590 0.47 -0.1 5,319,826 - BWI76_RS25590 0.51 -0.1 5,319,850 - BWI76_RS25590 0.62 -0.6 5,319,995 + +2.8 5,320,004 - -1.8 5,320,019 - +0.1 5,320,353 - BWI76_RS25600 0.49 -3.1
Or see this region's nucleotide sequence