Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16270 and BWI76_RS16275 are separated by 142 nucleotides BWI76_RS16275 and BWI76_RS16280 are separated by 197 nucleotides
BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540
_RS16270
BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329
_RS16275
BWI76_RS16280: BWI76_RS16280 - mannose-6-phosphate isomerase, at 3,378,527 to 3,379,705
_RS16280
Position (kb)
3376
3377
3378
3379 Strain fitness (log2 ratio)
-2
-1
0
1 at 3375.706 kb on + strand, within BWI76_RS16270 at 3376.030 kb on + strand, within BWI76_RS16270 at 3376.146 kb on + strand, within BWI76_RS16270 at 3376.166 kb on - strand, within BWI76_RS16270 at 3376.270 kb on + strand, within BWI76_RS16270 at 3376.367 kb on - strand, within BWI76_RS16270 at 3376.473 kb on + strand at 3376.531 kb on + strand at 3376.909 kb on - strand, within BWI76_RS16275 at 3377.840 kb on + strand, within BWI76_RS16275 at 3378.245 kb on + strand at 3378.253 kb on + strand at 3378.256 kb on - strand at 3378.326 kb on + strand at 3378.546 kb on - strand at 3378.658 kb on - strand, within BWI76_RS16280 at 3378.812 kb on + strand, within BWI76_RS16280 at 3378.916 kb on + strand, within BWI76_RS16280
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,375,706 + BWI76_RS16270 0.40 -1.9 3,376,030 + BWI76_RS16270 0.64 -0.9 3,376,146 + BWI76_RS16270 0.72 -0.6 3,376,166 - BWI76_RS16270 0.73 -0.2 3,376,270 + BWI76_RS16270 0.81 -0.4 3,376,367 - BWI76_RS16270 0.88 -0.3 3,376,473 + +0.9 3,376,531 + -0.7 3,376,909 - BWI76_RS16275 0.14 -0.5 3,377,840 + BWI76_RS16275 0.70 -1.8 3,378,245 + -1.5 3,378,253 + -0.3 3,378,256 - -2.3 3,378,326 + -0.1 3,378,546 - +0.0 3,378,658 - BWI76_RS16280 0.11 -0.1 3,378,812 + BWI76_RS16280 0.24 -0.1 3,378,916 + BWI76_RS16280 0.33 -0.2
Or see this region's nucleotide sequence