Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15640 and BWI76_RS15645 are separated by 3 nucleotides BWI76_RS15645 and BWI76_RS15650 overlap by 41 nucleotides
BWI76_RS15640: BWI76_RS15640 - hypothetical protein, at 3,268,333 to 3,269,598
_RS15640
BWI76_RS15645: BWI76_RS15645 - gp6, at 3,269,602 to 3,270,528
_RS15645
BWI76_RS15650: BWI76_RS15650 - gp5, at 3,270,488 to 3,271,837
_RS15650
Position (kb)
3269
3270
3271 Strain fitness (log2 ratio)
-2
-1
0
1 at 3268.771 kb on + strand, within BWI76_RS15640 at 3268.836 kb on - strand, within BWI76_RS15640 at 3269.021 kb on + strand, within BWI76_RS15640 at 3269.054 kb on - strand, within BWI76_RS15640 at 3269.192 kb on + strand, within BWI76_RS15640 at 3269.412 kb on + strand, within BWI76_RS15640 at 3269.420 kb on - strand, within BWI76_RS15640 at 3269.615 kb on - strand at 3269.616 kb on + strand at 3269.822 kb on + strand, within BWI76_RS15645 at 3269.978 kb on + strand, within BWI76_RS15645 at 3270.003 kb on + strand, within BWI76_RS15645 at 3270.065 kb on - strand, within BWI76_RS15645 at 3270.128 kb on - strand, within BWI76_RS15645 at 3270.207 kb on + strand, within BWI76_RS15645 at 3270.207 kb on + strand, within BWI76_RS15645 at 3270.454 kb on - strand at 3270.507 kb on + strand at 3270.612 kb on + strand at 3270.771 kb on - strand, within BWI76_RS15650 at 3271.112 kb on + strand, within BWI76_RS15650 at 3271.293 kb on + strand, within BWI76_RS15650 at 3271.323 kb on - strand, within BWI76_RS15650 at 3271.383 kb on - strand, within BWI76_RS15650
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,268,771 + BWI76_RS15640 0.35 -0.7 3,268,836 - BWI76_RS15640 0.40 -1.3 3,269,021 + BWI76_RS15640 0.54 -0.5 3,269,054 - BWI76_RS15640 0.57 -1.1 3,269,192 + BWI76_RS15640 0.68 -2.6 3,269,412 + BWI76_RS15640 0.85 -0.6 3,269,420 - BWI76_RS15640 0.86 -0.7 3,269,615 - -0.4 3,269,616 + -0.6 3,269,822 + BWI76_RS15645 0.24 -0.9 3,269,978 + BWI76_RS15645 0.41 -0.5 3,270,003 + BWI76_RS15645 0.43 +0.1 3,270,065 - BWI76_RS15645 0.50 -0.2 3,270,128 - BWI76_RS15645 0.57 -1.0 3,270,207 + BWI76_RS15645 0.65 -0.8 3,270,207 + BWI76_RS15645 0.65 -1.8 3,270,454 - -1.6 3,270,507 + -0.9 3,270,612 + -1.1 3,270,771 - BWI76_RS15650 0.21 -0.3 3,271,112 + BWI76_RS15650 0.46 -0.1 3,271,293 + BWI76_RS15650 0.60 -1.2 3,271,323 - BWI76_RS15650 0.62 -0.4 3,271,383 - BWI76_RS15650 0.66 -0.7
Or see this region's nucleotide sequence