Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10675 and BWI76_RS10680 are separated by 32 nucleotides BWI76_RS10680 and BWI76_RS10685 are separated by 103 nucleotides
BWI76_RS10675: BWI76_RS10675 - phosphoenolpyruvate--protein phosphotransferase, at 2,254,138 to 2,256,639
_RS10675
BWI76_RS10680: BWI76_RS10680 - tartrate dehydrogenase, at 2,256,672 to 2,257,790
_RS10680
BWI76_RS10685: BWI76_RS10685 - LysR family transcriptional regulator, at 2,257,894 to 2,258,850
_RS10685
Position (kb)
2256
2257
2258 Strain fitness (log2 ratio)
-1
0
1 at 2255.908 kb on - strand, within BWI76_RS10675 at 2256.226 kb on - strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.245 kb on + strand, within BWI76_RS10675 at 2256.253 kb on - strand, within BWI76_RS10675 at 2256.299 kb on + strand, within BWI76_RS10675 at 2256.634 kb on - strand at 2257.058 kb on + strand, within BWI76_RS10680 at 2257.097 kb on + strand, within BWI76_RS10680 at 2257.667 kb on + strand, within BWI76_RS10680 at 2257.928 kb on + strand at 2257.928 kb on + strand at 2258.359 kb on - strand, within BWI76_RS10685 at 2258.458 kb on + strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.466 kb on - strand, within BWI76_RS10685 at 2258.537 kb on + strand, within BWI76_RS10685
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,255,908 - BWI76_RS10675 0.71 -0.4 2,256,226 - BWI76_RS10675 0.83 -0.3 2,256,245 + BWI76_RS10675 0.84 +0.2 2,256,245 + BWI76_RS10675 0.84 -0.4 2,256,253 - BWI76_RS10675 0.85 +0.8 2,256,299 + BWI76_RS10675 0.86 -0.1 2,256,634 - -0.5 2,257,058 + BWI76_RS10680 0.34 +0.2 2,257,097 + BWI76_RS10680 0.38 +0.8 2,257,667 + BWI76_RS10680 0.89 +0.3 2,257,928 + +0.0 2,257,928 + -0.0 2,258,359 - BWI76_RS10685 0.49 +0.7 2,258,458 + BWI76_RS10685 0.59 +0.1 2,258,466 - BWI76_RS10685 0.60 +0.1 2,258,466 - BWI76_RS10685 0.60 +0.2 2,258,466 - BWI76_RS10685 0.60 +0.5 2,258,537 + BWI76_RS10685 0.67 -0.0
Or see this region's nucleotide sequence