Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08720 and BWI76_RS08725 overlap by 4 nucleotides BWI76_RS08725 and BWI76_RS08730 are separated by 53 nucleotides BWI76_RS08730 and BWI76_RS08735 are separated by 143 nucleotides
BWI76_RS08720: BWI76_RS08720 - imidazolonepropionase, at 1,836,606 to 1,837,823
_RS08720
BWI76_RS08725: BWI76_RS08725 - Formiminoglutamase (EC 3.5.3.8) (from data) , at 1,837,820 to 1,838,776
_RS08725
BWI76_RS08730: BWI76_RS08730 - histidine utilization repressor, at 1,838,830 to 1,839,555
_RS08730
BWI76_RS08735: BWI76_RS08735 - Urocanate hydratase (EC 4.2.1.49) (from data) , at 1,839,699 to 1,841,387
_RS08735
Position (kb)
1837
1838
1839 Strain fitness (log2 ratio)
-2
-1
0
1 at 1837.299 kb on + strand, within BWI76_RS08720 at 1837.602 kb on + strand, within BWI76_RS08720 at 1837.653 kb on - strand, within BWI76_RS08720 at 1837.885 kb on - strand at 1838.181 kb on + strand, within BWI76_RS08725 at 1838.229 kb on + strand, within BWI76_RS08725 at 1838.484 kb on - strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725 at 1839.414 kb on - strand, within BWI76_RS08730 at 1839.414 kb on - strand, within BWI76_RS08730 at 1839.482 kb on - strand, within BWI76_RS08730 at 1839.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,837,299 + BWI76_RS08720 0.57 -0.4 1,837,602 + BWI76_RS08720 0.82 +0.3 1,837,653 - BWI76_RS08720 0.86 -2.0 1,837,885 - -0.5 1,838,181 + BWI76_RS08725 0.38 -0.5 1,838,229 + BWI76_RS08725 0.43 -0.5 1,838,484 - BWI76_RS08725 0.69 -0.2 1,838,668 + BWI76_RS08725 0.89 -1.5 1,838,668 + BWI76_RS08725 0.89 -1.1 1,839,414 - BWI76_RS08730 0.80 -0.7 1,839,414 - BWI76_RS08730 0.80 -0.3 1,839,482 - BWI76_RS08730 0.90 -0.5 1,839,640 - +0.3
Or see this region's nucleotide sequence