Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01165 and BWI76_RS01170 are separated by 3 nucleotides BWI76_RS01170 and BWI76_RS01175 are separated by 21 nucleotides BWI76_RS01175 and BWI76_RS01180 overlap by 4 nucleotides
BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822
_RS01165
BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995
_RS01170
BWI76_RS01175: BWI76_RS01175 - uroporphyrinogen-III synthase, at 240,017 to 240,757
_RS01175
BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695
_RS01180
Position (kb) 238
239
240 Strain fitness (log2 ratio) -1
0
1 at 237.830 kb on + strand, within BWI76_RS01165 at 237.855 kb on + strand, within BWI76_RS01165 at 237.889 kb on - strand, within BWI76_RS01165 at 238.154 kb on + strand, within BWI76_RS01165 at 238.162 kb on - strand, within BWI76_RS01165 at 238.230 kb on + strand, within BWI76_RS01165 at 238.564 kb on + strand, within BWI76_RS01165 at 238.944 kb on - strand, within BWI76_RS01170 at 239.119 kb on - strand, within BWI76_RS01170 at 239.155 kb on + strand, within BWI76_RS01170 at 239.528 kb on + strand, within BWI76_RS01170 at 239.545 kb on + strand, within BWI76_RS01170 at 239.939 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 237,830 + BWI76_RS01165 0.17 +0.4 237,855 + BWI76_RS01165 0.19 -1.2 237,889 - BWI76_RS01165 0.22 +0.1 238,154 + BWI76_RS01165 0.44 -0.4 238,162 - BWI76_RS01165 0.45 -0.4 238,230 + BWI76_RS01165 0.50 -0.7 238,564 + BWI76_RS01165 0.78 -0.5 238,944 - BWI76_RS01170 0.10 -0.3 239,119 - BWI76_RS01170 0.25 -0.2 239,155 + BWI76_RS01170 0.28 -1.3 239,528 + BWI76_RS01170 0.60 -1.1 239,545 + BWI76_RS01170 0.61 -0.3 239,939 + -1.3
Or see this region's nucleotide sequence