Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00005 and BWI76_RS00010 are separated by 28 nucleotides BWI76_RS00010 and BWI76_RS00015 are separated by 138 nucleotides
BWI76_RS00005: BWI76_RS00005 - DNA gyrase subunit B, at 1 to 2,415
_RS00005
BWI76_RS00010: BWI76_RS00010 - DNA replication and repair protein RecF, at 2,444 to 3,517
_RS00010
BWI76_RS00015: BWI76_RS00015 - DNA polymerase III subunit beta, at 3,656 to 4,756
_RS00015
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.540 kb on - strand at 2.648 kb on + strand, within BWI76_RS00010 at 2.713 kb on + strand, within BWI76_RS00010 at 2.831 kb on + strand, within BWI76_RS00010 at 2.860 kb on + strand, within BWI76_RS00010 at 2.933 kb on + strand, within BWI76_RS00010 at 3.030 kb on + strand, within BWI76_RS00010 at 3.120 kb on + strand, within BWI76_RS00010 at 3.167 kb on + strand, within BWI76_RS00010 at 3.256 kb on - strand, within BWI76_RS00010 at 3.310 kb on + strand, within BWI76_RS00010 at 3.318 kb on - strand, within BWI76_RS00010 at 3.318 kb on - strand, within BWI76_RS00010 at 3.318 kb on - strand, within BWI76_RS00010 at 3.318 kb on - strand, within BWI76_RS00010 at 3.318 kb on - strand, within BWI76_RS00010 at 3.529 kb on + strand at 3.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,540 - +0.1 2,648 + BWI76_RS00010 0.19 -1.3 2,713 + BWI76_RS00010 0.25 -1.1 2,831 + BWI76_RS00010 0.36 -0.8 2,860 + BWI76_RS00010 0.39 -0.3 2,933 + BWI76_RS00010 0.46 -0.1 3,030 + BWI76_RS00010 0.55 +1.6 3,120 + BWI76_RS00010 0.63 -0.6 3,167 + BWI76_RS00010 0.67 -0.7 3,256 - BWI76_RS00010 0.76 -0.3 3,310 + BWI76_RS00010 0.81 -0.1 3,318 - BWI76_RS00010 0.81 -1.2 3,318 - BWI76_RS00010 0.81 -0.8 3,318 - BWI76_RS00010 0.81 -2.0 3,318 - BWI76_RS00010 0.81 -0.4 3,318 - BWI76_RS00010 0.81 -0.9 3,529 + -0.6 3,561 - +0.7
Or see this region's nucleotide sequence