Experiment: Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_420 and HP15_421 are separated by 26 nucleotides HP15_421 and HP15_422 overlap by 1 nucleotides HP15_422 and HP15_423 overlap by 11 nucleotides
HP15_420: HP15_420 - type II secretory pathway, pseudopilin PulG, at 416,110 to 416,577
_420
HP15_421: HP15_421 - MSHA pilin protein MshD, at 416,604 to 417,062
_421
HP15_422: HP15_422 - protein containing prepilin-type cleavage/methylation, N-terminal domains, at 417,062 to 417,847
_422
HP15_423: HP15_423 - conserved hypothetical protein, at 417,837 to 418,250
_423
Position (kb) 416
417
418 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 415.635 kb on - strand at 415.731 kb on + strand at 415.887 kb on + strand at 415.889 kb on + strand at 415.927 kb on - strand at 415.960 kb on - strand at 416.075 kb on + strand at 416.227 kb on + strand, within HP15_420 at 416.399 kb on - strand, within HP15_420 at 416.511 kb on + strand, within HP15_420 at 416.591 kb on - strand at 416.708 kb on - strand, within HP15_421 at 416.729 kb on + strand, within HP15_421 at 416.729 kb on + strand, within HP15_421 at 416.912 kb on - strand, within HP15_421 at 416.940 kb on + strand, within HP15_421 at 416.940 kb on + strand, within HP15_421 at 417.078 kb on + strand at 417.168 kb on + strand, within HP15_422 at 417.233 kb on + strand, within HP15_422 at 417.242 kb on + strand, within HP15_422 at 417.242 kb on + strand, within HP15_422 at 417.299 kb on - strand, within HP15_422 at 417.299 kb on - strand, within HP15_422 at 417.327 kb on + strand, within HP15_422 at 417.421 kb on - strand, within HP15_422 at 417.434 kb on - strand, within HP15_422 at 417.489 kb on - strand, within HP15_422 at 417.750 kb on - strand, within HP15_422 at 417.771 kb on + strand at 417.771 kb on + strand at 417.841 kb on + strand at 417.849 kb on - strand at 417.874 kb on + strand at 418.005 kb on + strand, within HP15_423
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Synechococcus str. WH8102 10:1 starting OD to HP15 remove 415,635 - +1.1 415,731 + +1.3 415,887 + +2.5 415,889 + +1.6 415,927 - -0.1 415,960 - +1.1 416,075 + +0.8 416,227 + HP15_420 0.25 +0.5 416,399 - HP15_420 0.62 +1.7 416,511 + HP15_420 0.86 +0.3 416,591 - +0.0 416,708 - HP15_421 0.23 +1.1 416,729 + HP15_421 0.27 +1.8 416,729 + HP15_421 0.27 +3.2 416,912 - HP15_421 0.67 +1.6 416,940 + HP15_421 0.73 +2.7 416,940 + HP15_421 0.73 +4.8 417,078 + +2.0 417,168 + HP15_422 0.13 -0.3 417,233 + HP15_422 0.22 +3.0 417,242 + HP15_422 0.23 +1.0 417,242 + HP15_422 0.23 +0.9 417,299 - HP15_422 0.30 +2.5 417,299 - HP15_422 0.30 +0.4 417,327 + HP15_422 0.34 +0.2 417,421 - HP15_422 0.46 +2.3 417,434 - HP15_422 0.47 +2.3 417,489 - HP15_422 0.54 +3.0 417,750 - HP15_422 0.88 +0.2 417,771 + +0.3 417,771 + +2.3 417,841 + +0.6 417,849 - -0.1 417,874 + +0.7 418,005 + HP15_423 0.41 +2.0
Or see this region's nucleotide sequence